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Indian J Exp Biol ; 2014 Nov; 52(11): 1090-1097
Artículo en Inglés | IMSEAR | ID: sea-153795

RESUMEN

The present study describes a novel and simple vaccination strategy that involve culturing of M. tuberculosis in the macrophage cells. Isolation of phagosome from macrophage (cell line J774) infected with M. tuberculosis (H37) and M. bovis (BCG) at early and late phase of infection was done ensuing the identification and characterization of these phagosome. In vitro study of apoptosis induced by phagosome infected with (H37) and (BCG) was performed. The vaccine candidate with H37 MOI- 1:10 at 3 h, MOI- 1:20 at 1, 1.5, 2.5 and 3 h and BCG MOI- 1:20 at 3.5 h showed percentage apoptosis as 38.64, 39.93, 34.66, 22.56,34.59 and 37.81% respectively. The results designates that macrophages provide cellular niche during infection and illustrate considerable immunogenic property. Novel antigens expressed or secreted by H37 in infected macrophages can provide evidence to be a successful vaccine candidate as it endures enhanced immune response than BCG.


Asunto(s)
Animales , Antígenos Bacterianos/inmunología , Apoptosis , Línea Celular Tumoral , Medios de Cultivo , Fragmentación del ADN , Linfoma no Hodgkin/patología , Macrófagos/microbiología , Ratones , Ratones Endogámicos BALB C , Mycobacterium bovis/crecimiento & desarrollo , Mycobacterium bovis/inmunología , Mycobacterium tuberculosis/crecimiento & desarrollo , Mycobacterium tuberculosis/inmunología , Fagosomas/inmunología , Fagosomas/microbiología , Vacunas contra la Tuberculosis/inmunología , Vacunas contra la Tuberculosis/aislamiento & purificación
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