Your browser doesn't support javascript.
loading
Mostrar: 20 | 50 | 100
Resultados 1 - 2 de 2
Filtrar
Añadir filtros








Intervalo de año
1.
Indian J Biochem Biophys ; 2011 Aug; 48(4): 275-282
Artículo en Inglés | IMSEAR | ID: sea-135329

RESUMEN

Baicalein (5, 6, 7-trihydroxy-2-phenyl-4H-1-benzopyran-4-one), a naturally occurring flavone present in some of the medicinal plants is known for its potential therapeutic effects, such as cardioprotective, anticancer and anti-inflammatory properties. However, detailed role and mechanisms behind its protective properties against different generators for oxidative stress have not been examined. In the present study, we investigated the possible protective ability of baicalein against the membrane damage caused by reactive oxygen species (ROS) and reactive nitrogen species (RNS) and the mechanisms involved using pulse radiolysis technique. Baicalein offered efficient protection even at a concentration of 10 M towards membrane damage caused by lipid peroxidation induced by the -radiation, peroxyl radicals, ascorbate-Fe2+ and peroxynitrite in rat liver mitochondria and heart homogenate. To elucidate its reaction mechanisms with biologically relevant radicals, transient absorption spectroscopy employing pulse radiolysis technique was used. Baicalein showed fairly high rate constants (3.7 × 109, 1.3 × 109 and 8.0 × 108 dm3 mol-1 s-1 for hydroxyl, azidyl and alkylchloroperoxyl radicals, respectively), suggesting that baicalein can act as an effective scavenger of these radicals. In each case, the phenoxyl radical of baicalein was generated. Thus, it was evident that the phenolic moiety of baicalein was responsible for the free radical scavenging process. Baicalein also reacts with linoleic acid peroxyl radical (LOO·), indicating its ability to act as a chain breaking antioxidant. Peroxynitrite-mediated radicals were shown to be reactive towards baicalein and the bimolecular rate constants were 2.5 × 107 and 3 × 108 dm3 mol-1 s-1 for ·NO2 and CO3·- radicals, respectively. In conclusion, our results revealed the potential of baicalein in protecting mitochondrial membrane against oxidative damage induced by the four different agents. We propose that the protective effect is mediated via scavenging of primary and secondary radicals generated during oxidative stress.


Asunto(s)
Animales , Membrana Celular/efectos de los fármacos , Femenino , Flavanonas/química , Flavanonas/farmacología , Radicales Libres , Corazón/efectos de los fármacos , Mitocondrias Hepáticas/efectos de los fármacos , Ratas , Ratas Wistar , Especies Reactivas de Oxígeno/metabolismo
2.
Indian J Exp Biol ; 2008 Nov; 46(11): 783-7
Artículo en Inglés | IMSEAR | ID: sea-62123

RESUMEN

FtsZ plays an important role in bacterial cell division by polymerizing to form the Z ring at the site of cytokinesis. Phytochemicals are known to disrupt bacterial cell division through inhibition of FtsZ assembly. In the present study phytochemicals like eugenol, trans-cinnamic acid, 4-formyl cinnamic acid, naringenin and caffeic acid were were tested for their potential to inhibit cell division. Effect of these antimicrobial compounds on the growth of E. coli was determined and the inhibition of FtsZ assembly in vitro was investigated. The present study revealed trans-cinnamic acid as the most potent inhibitor of FtsZ assembly.


Asunto(s)
Antibacterianos/farmacología , Proteínas Bacterianas/metabolismo , Ácidos Cafeicos/química , División Celular , Cinamatos/química , Proteínas del Citoesqueleto/metabolismo , Evaluación Preclínica de Medicamentos , Escherichia coli/metabolismo , Eugenol/química , Flavanonas/química , Luz , Modelos Biológicos , Nefelometría y Turbidimetría , Polímeros/química , Proteínas Recombinantes/química , Dispersión de Radiación
SELECCIÓN DE REFERENCIAS
DETALLE DE LA BÚSQUEDA