RESUMEN
ABSTRACT Vitellibacter aquimaris D-24T (=KCTC 42708T = DSM 101732T), a halophilic marine bacterium, was isolated from seawater collected from Desaru beach, Malaysia. Here, we present the draft genome sequence of D-24T with a genome size of approximately 3.1 Mbp and G + C content of 39.93%. The genome of D-24T contains genes involved in reducing a potent greenhouse gas (N2O) in the environment and the degradation of proteinaceous compounds. Genome availability will provide insights into potential biotechnological and environmental applications of this bacterium.
Asunto(s)
Agua de Mar/microbiología , Genoma Bacteriano , Flavobacteriaceae/genética , Filogenia , Composición de Base , ADN Bacteriano/genética , Datos de Secuencia Molecular , Secuencia de Bases , Flavobacteriaceae/aislamiento & purificación , Flavobacteriaceae/clasificación , MalasiaRESUMEN
ABSTRACT Freezing temperatures are a major challenge for life at the poles. Decreased membrane fluidity, uninvited secondary structure formation in nucleic acids, and protein cold-denaturation all occur at cold temperatures. Organisms adapted to polar regions possess distinct mechanisms that enable them to survive in extremely cold environments. Among the cold-induced proteins, cold shock protein (Csp) family proteins are the most prominent. A gene coding for a Csp-family protein, cspB, was cloned from an arctic bacterium, Polaribacter irgensii KOPRI 22228, and overexpression of cspB greatly increased the freeze-survival rates of Escherichia coli hosts, to a greater level than any previously reported Csp. It also suppressed the cold-sensitivity of an E. coli csp-quadruple deletion strain, BX04. Sequence analysis showed that this protein consists of a unique domain at its N-terminal end and a well conserved cold shock domain at its C-terminal end. The most common mechanism of Csp function in cold adaption is melting of the secondary structures in RNA and DNA molecules, thus facilitating transcription and translation at low temperatures. P. irgensii CspB bound to oligo(dT)-cellulose resins, suggesting single-stranded nucleic acid-binding activity. The unprecedented level of freeze-tolerance conferred by P. irgensii CspB suggests a crucial role for this protein in survival in polar environments.
Asunto(s)
Proteínas Bacterianas/metabolismo , Flavobacteriaceae/fisiología , Proteínas y Péptidos de Choque por Frío/metabolismo , Regiones Árticas , Proteínas Bacterianas/genética , Regulación Bacteriana de la Expresión Génica , Frío , Ecosistema , Flavobacteriaceae/aislamiento & purificación , Flavobacteriaceae/genética , Proteínas y Péptidos de Choque por Frío/genéticaRESUMEN
Members of the genus Myroides are aerobic Gram-negative bacteria that are common in environmental sources, but are not components of the normal human microflora. Myroides organisms behave as low-grade opportunistic pathogens, causing infections in severely immunocompromised patients and rarely, in immunocompetent hosts. A case of Myroides odoratimimus cellulitis following a pig bite in an immunocompetent child is presented, and the medical literature on Myroides spp. soft tissue infections is reviewed.
Asunto(s)
Adolescente , Animales , Humanos , Masculino , Mordeduras y Picaduras/complicaciones , Celulitis (Flemón)/microbiología , Infecciones por Flavobacteriaceae/microbiología , Flavobacteriaceae/aislamiento & purificación , Infecciones de los Tejidos Blandos/microbiología , Celulitis (Flemón)/diagnóstico , Infecciones por Flavobacteriaceae/diagnóstico , Inmunocompetencia , Porcinos , Infecciones de los Tejidos Blandos/diagnósticoRESUMEN
Here we report a case of acalculus cholecystitis, which presented with features of obstructive jaundice of one-week duration. The patient underwent cholecystectomy and bile grew a mixed culture of Oerskovia turbata and Myroides spp. Being a rare isolate, characteristic features of the former are described in this report. The patient recovered without any complication.