RESUMEN
Fucoidan is a sulfated polysaccharide derived from brown seaweed, including Fucus vesiculosus. This compound is known to have immunostimulatory effects on various types of immune cells including macrophages and dendritic cells. A recent study described the application of fucoidan as a vaccine adjuvant. Vaccination is regarded as the most efficient prophylactic method for preventing harmful or epidemic diseases. To increase vaccine efficacy, effective adjuvants are needed. In the present study, we determined whether fucoidan can function as an adjuvant using vaccine antigens. Flow cytometric analysis revealed that fucoidan increases the expression of the activation markers major histocompatibility complex class II, cluster of differentiation (CD)25, and CD69 in spleen cells. In combination with Bordetella bronchiseptica antigen, fucoidan increased the viability and tumor necrosis factor-alpha production of spleen cells. Furthermore, fucoidan increased the in vivo production of antigen-specific antibodies in mice inoculated with Mycoplasma hyopneumoniae antigen. Overall, this study has provided valuable information about the use of fucoidan as a vaccine adjuvant.
Asunto(s)
Animales , Femenino , Ratones , Adyuvantes Inmunológicos/farmacología , Antígenos Bacterianos/inmunología , Vacunas Bacterianas/administración & dosificación , Biomarcadores/metabolismo , Bordetella bronchiseptica/inmunología , Células Cultivadas , Citocinas/metabolismo , Citometría de Flujo , Fucus/química , Regulación de la Expresión Génica/efectos de los fármacos , Ratones Endogámicos BALB C , Mycoplasma hyopneumoniae/inmunología , Polisacáridos/farmacología , Bazo/metabolismoRESUMEN
Fucoidan is an active component of seaweed, which inhibits proliferation and induces apoptosis of several tumor cells while the detailed mechanisms underlying this process are still not clear. In this study, the effect of Fucoidan on the proliferation and apoptosis of human breast cancer MCF-7 cells and the molecular mechanism of Fucoidan action were investigated. Viable cell number of MCF-7 cells was decreased by Fucoidan treatment in a dose-dependent manner as measured by MTT assay. Fucoidan treatment resulted in G1 phase arrest of MCF-7 cells as revealed by flow cytometry, which was associated with the decrease in the gene expression of cyclin D1 and CDK-4. Annexin V/PI staining results showed that the number of apoptotic cells was associated with regulation of cytochrome C, caspase-8, Bax and Bcl-2 at transcriptional and translational levels. Both morphologic observation and Hoechst 33258 assay results confirmed the pro-apoptotic effect of Fucoidan. Meanwhile, the ROS production was also increased by Fucoidan treatment, which suggested that Fucoidan induced oxidative damage in MCF-7 cells. The results of present study demonstrated that Fucoidan could induce G1 phase arrest and apoptosis in MCF-7 cells through regulating the cell cycle and apoptosis-related genes or proteins expression, and ROS generation is also involved in these processes.
Asunto(s)
Humanos , Antineoplásicos , Química , Farmacología , Apoptosis , Genética , Western Blotting , Neoplasias de la Mama , Genética , Metabolismo , Patología , Caspasa 8 , Genética , Metabolismo , Caspasas , Genética , Metabolismo , Proliferación Celular , Tamaño de la Célula , Ciclina D1 , Genética , Metabolismo , Quinasa 4 Dependiente de la Ciclina , Genética , Metabolismo , Citocromos c , Genética , Metabolismo , Relación Dosis-Respuesta a Droga , Fucus , Química , Puntos de Control de la Fase G1 del Ciclo Celular , Genética , Regulación Neoplásica de la Expresión Génica , Células MCF-7 , Microscopía Fluorescente , Estructura Molecular , Polisacáridos , Química , Farmacología , Proteínas Proto-Oncogénicas c-bcl-2 , Genética , Metabolismo , Especies Reactivas de Oxígeno , Metabolismo , Reacción en Cadena de la Polimerasa de Transcriptasa Inversa , Transducción de Señal , Proteína X Asociada a bcl-2 , Genética , MetabolismoRESUMEN
BACKGROUND: Fucoidan is a highly sulfated glycosaminoglycan, which has a molecular structure similar to that of heparin. The antithrombotic effects of fucoidan in vitro have been widely reported, but its antithrombotic effects in vivo as well as its other biological properties in vitro have not been well investigated. METHODS: This study investigated the effects and mechanism of fucoidan from Fucus vesiculosus on thrombosis both in vitro and in vivo. A ferric chloride-induced mouse carotid artery thrombosis model was used to determine the antithrombotic effects of fucoidan in vivo. Additionally, changes in the levels of proinflammatory cytokines and chemokines were examined in vascular cells treated with fucoidan. RESULTS: In vivo studies employing a ferric chloride-induced mouse carotid artery thrombosis model indicated that fucoidan had a stronger antithrombotic activity than heparin. Further, vascular cells treated with fucoidan demonstrated a decrease in proinflammatory cytokine and chemokine production as well as inhibition of proliferation. CONCLUSION: The major findings of this study showed that fucoidan has a stronger antithrombotic effect than heparin in vivo and that fucoidan has an inhibitory effect on proinflammatory cytokine production and proliferation of vascular cells.
Asunto(s)
Animales , Ratones , Trombosis de las Arterias Carótidas , Quimiocinas , Citocinas , Fucus , Glicosaminoglicanos , Heparina , Estructura Molecular , Polisacáridos , TrombosisRESUMEN
Los fitoflagelados son un grupo heterogéneo de flagelados autotróficos, heterotróficos y mixotróficos, con importancia ecológica para los niveles tróficos en diferentes ecosistemas. Los fitoflagelados en costas del Pacífico mexicano (y en Latinoamérica en términos generales) son virtualmente desconocidos, sólo se tienen pocos registros. El estudio de los fitoflagelados requiere de métodos complicados de recolección y análisis. Esta es, probablemente, la causa de la escasez de conocimiento de este grupo en áreas tropicales y subtropicales. Material recientemente recolectado a lo largo del Pacífico mexicano sirvió para el estudio de fitoflagelados marinos, incluyendo algunos tóxicos y potencialmente tóxicos. Se usaron muestras de plancton filtradas por gravedad y con bomba de vacío utilizando diferentes métodos de fijación y análisis. Se registran aquellas especies presentes o con posibilidad de estarlo que son potencialmente nocivas para el ecosistema marino pertenecientes a los Phyla Euglenophyta, Heterokontophyta y Haptophyta. Estas especies están distribuidas en el plancton, en aguas oceánicas y costeras
The phytoflagellates are a heterogeneous group of autotrophic, heterotrophic and mixothrophic flagellates of trophic importance in several ecosystems. As in the rest of Latin America, the phytoflagellates that occur in the Mexican Pacific coasts are virtually unknown except for a few records. Their study require complicated collection and analysis methods, a probable cause for the scarce knowledge of this group in tropical and subtropical areas. Material recently collected from various localities along the Mexican Pacific coasts was used to study phytoflagellates, including toxic and potentially toxic species. Plankton samples were treated by gravity and pump filtration, using different methods for fixation and analysis. The phyla Euglenophyta, Heterokontophyta and Haptophyta were found. They occur as plankton in oceanic and shallow coastal waters