RESUMEN
Patients transplanted from solid organs have an increased risk of cancer, especially lymphomas. Lymphomas correspond to 4 to 5% of malignant neoplasms in the general population and in solid organ transplant patients it reaches an incidence of 21%. The incidence of non-Hodgkin lymphomas is 10 times higher than in the non-transplanted population. We report the case of a 68-year-old man with a kidney transplant who 6 years after transplantation, developed a non-Hodgkin diffuse large cells B lymphoma with lymph node and pulmonary involvement, with markers of very poor prognosis (triple MYC expressor, BCL2 and BCL6). and its evolution with chemotherapy with DA R EPOCH.
Asunto(s)
Humanos , Masculino , Anciano , Linfoma no Hodgkin , Linfoma de Células B Grandes Difuso/genética , Vincristina/uso terapéutico , Linfoma no Hodgkin/etiología , Linfoma no Hodgkin/tratamiento farmacológico , Prednisona/uso terapéutico , Protocolos de Quimioterapia Combinada Antineoplásica/uso terapéutico , Doxorrubicina/uso terapéutico , Biomarcadores de Tumor/genética , Genes myc/genética , Linfoma de Células B Grandes Difuso/etiología , Linfoma de Células B Grandes Difuso/tratamiento farmacológico , Trasplante de Riñón/efectos adversos , Proteínas Proto-Oncogénicas c-bcl-2/genética , Ciclofosfamida/uso terapéutico , Proteínas Proto-Oncogénicas c-bcl-6/genética , Etopósido/uso terapéuticoRESUMEN
BACKGROUND: The aberrant expression of microRNAs (miRNAs) has been found in various types of cancer. miR-205 was reported to be upregulated in laryngeal squamous cell carcinoma (LSCC) tissues, however, the mechanisms by which miR-205 functions as a regulator of LSCC are largely unknown. RESULTS: In this study, Real-time qPCR and Western blot assay showed that expression of miR-205 was upregulated and expression of cyclin-dependent kinase 2-associated protein 1 (CDK2AP1) was downregulated in LSCC tissues. The expression levels of miR-205 were negatively related to those of CDK2AP1 in LSCC tissues and cell lines. Moreover, we found that miR-205 was the upstream regulator of CDK2AP1 and could suppress the CDK2AP1 expression in LSCC cells. 3-(4,5-dimethylthiazal-2-yl)-2,5-diphenyl-tetrazolium bromide assays and transwell invasion assay were performed to test the proliferation and invasion of LSCC cells. Gelatin zymography was used to detect the activity of MMP2 and MMP9. CDK2AP1, c-Myc and CyclinD1 expression in cells was assessed with Western blotting. We found that miR-205 was the upstream regulator of CDK2AP1 and could suppress the expression of CDK2AP1 in LSCC cells. In addition, miR-205 significantly induced cell proliferation and invasion by suppressing CDK2AP1 expression. Consistent with miR-205 inhibitors, overexpressed CDK2AP1 suppressed the activity of MMP2 and MMP9 and c-Myc and CyclinD1 expression in LSCC cells. CONCLUSION: These findings help us to better elucidate the molecular mechanisms of LSCC progression and provide a new theoretical basis to further investigate miR-205 as a potential biomarker and a promising approach for LSCC treatment.
Asunto(s)
Humanos , Supresión Genética/genética , Carcinoma de Células Escamosas/patología , Neoplasias Laríngeas/patología , Proteínas Supresoras de Tumor/genética , MicroARNs/genética , Proliferación Celular/genética , Carcinoma de Células Escamosas/enzimología , Biomarcadores de Tumor , Regulación hacia Abajo , Regulación Neoplásica de la Expresión Génica , Western Blotting , Genes myc/genética , Ciclina D1/metabolismo , Metaloproteinasa 2 de la Matriz/metabolismo , Metaloproteinasa 9 de la Matriz/metabolismo , Proteínas Supresoras de Tumor/metabolismo , MicroARNs/metabolismo , Células Hep G2 , Cultivo Primario de Células , Reacción en Cadena en Tiempo Real de la Polimerasa , Invasividad Neoplásica/genéticaRESUMEN
Gastric cancer is the fourth most frequent type of cancer and the second cause of cancer mortality worldwide. The genetic alterations described so far for gastric carcinomas include amplifications and mutations of the c-ERBB2, KRAS, MET, TP53, and c-MYC genes. Chromosomal instability described for gastric cancer includes gains and losses of whole chromosomes or parts of them and these events might lead to oncogene overexpression, showing the need for a better understanding of the cytogenetic aspects of this neoplasia. Very few gastric carcinoma cell lines have been isolated. The establishment and characterization of the biological properties of gastric cancer cell lines is a powerful tool to gather information about the evolution of this malignancy, and also to test new therapeutic approaches. The present study characterized cytogenetically PG-100, the first commercially available gastric cancer cell line derived from a Brazilian patient who had a gastric adenocarcinoma, using GTG banding and fluorescent in situ hybridization to determine MYC amplification. Twenty metaphases were karyotyped; 19 (95 percent) of them presented chromosome 8 trisomy, where the MYC gene is located, and 17 (85 percent) presented a deletion in the 17p region, where the TP53 is located. These are common findings for gastric carcinomas, validating PG100 as an experimental model for this neoplasia. Eighty-six percent of 200 cells analyzed by fluorescent in situ hybridization presented MYC overexpression. Less frequent findings, such as 5p deletions and trisomy 16, open new perspectives for the study of this tumor.
Asunto(s)
Humanos , Adenocarcinoma/genética , Línea Celular Tumoral , Genes myc/genética , Neoplasias Gástricas/genética , Adenocarcinoma/patología , Brasil , Análisis Citogenético , Amplificación de Genes , Cariotipificación , Neoplasias Gástricas/patologíaRESUMEN
Dexamethasone converts pluripotent pancreatic AR42J cells into exocrine cells expressing digestive enzymes. In order to address molecular mechanism of this differentiation, we have investigated the role of mitogen-activated protein (MAP) kinase pathway and gene expressions of p21(waf1/cip1)and nuclear oncogenes (c-fos and c-myc) during AR42J cell differentiation. Dexamethasone markedly increased the intracellular and secreted amylase contents as well as its mRNA level. However, cell growth and DNA content were significantly decreased. With these phenotypic changes, AR42J cells induced transient mRNA expression of p21(waf1/cip1)gene, which reached maximal level by 6 h and then declined gradually toward basal state. In contrast to p21(waf1/cip1), c-fos gene expression was transiently inhibited by 6 h and then recovered to basal level by 24 h. Increased c-myc expression detected after 3 h, peaked by 12 h, and remained elevated during the rest of observation. Dexamethasone inhibited epidermal growth factor-induced phosphorylation of extracellular signal regulated kinase. Inhibition of MAP kinase pathway by PD98059 resulted in further elevation of the dexamethasone-induced amylase mRNA and p21(waf1/cip1)gene expression. These results suggest that p21(waf1/cip1)and nuclear oncogenes are involved in dexamethasone-induced differentiation and inhibition of MAP kinase pathway accelerates the conversion of undifferentiated AR42J cells into amylase-secreting exocrine cells.
Asunto(s)
Animales , Ratas , Amilasas/genética , Diferenciación Celular/efectos de los fármacos , División Celular/efectos de los fármacos , Línea Celular Tumoral , Ciclinas/genética , Dexametasona/farmacología , Regulación de la Expresión Génica/efectos de los fármacos , Genes fos/genética , Genes myc/genética , Sistema de Señalización de MAP Quinasas/efectos de los fármacos , Proteínas Quinasas Activadas por Mitógenos/metabolismo , Páncreas/citología , ARN Mensajero/genéticaRESUMEN
SV40 large T antigen, a viral oncoprotein, is known to immortalize human diploid fibroblast by soaking up cellular RB and p53, but its frequency is extremely low. Additional genetic alteration is necessary for single-step immortalization. We attempted to find out what this alteration is by overexpressing cellular signal mediator genes; c-myc and cyclin D frequently amplified in many cancer cells. Overexpression of cyclin D did not affect the immortalization, but, overexpression of c-myc along with T antigen could immortalize normal human diploid fibroblast. Several cellular markers tested during immortalization process showed that p21, a cyclin-dependent kinase inhibitor and a marker of cellular senescence, disappeared in the life span-extended cells by T antigen and in the immortalized cells by c-myc. p21 was, however, elevated in the senescent cells and in the cells of crisis. Interestingly, p16 was upregulated whenever T antigen is overexpressed. Telomerase activity was also activated only in the immortalized cells. These results suggest that overexpression of c-myc contributes to immortalization of human diploid fibroblast by activating telomerase activity and suppressing p21 activity.
Asunto(s)
Humanos , Antígenos Transformadores de Poliomavirus/genética , Biomarcadores , Senescencia Celular/genética , Transformación Celular Viral , Células Cultivadas , Ciclinas/metabolismo , Diploidia , Fibroblastos/metabolismo , Genes myc/genética , Inhibidor p16 de la Quinasa Dependiente de Ciclina/metabolismo , Virus 40 de los Simios/genética , Telomerasa/metabolismoRESUMEN
SV40 large T antigen, a viral oncoprotein, is known to immortalize human diploid fibroblast by soaking up cellular RB and p53, but its frequency is extremely low. Additional genetic alteration is necessary for single-step immortalization. We attempted to find out what this alteration is by overexpressing cellular signal mediator genes; c-myc and cyclin D frequently amplified in many cancer cells. Overexpression of cyclin D did not affect the immortalization, but, overexpression of c-myc along with T antigen could immortalize normal human diploid fibroblast. Several cellular markers tested during immortalization process showed that p21, a cyclin-dependent kinase inhibitor and a marker of cellular senescence, disappeared in the life span-extended cells by T antigen and in the immortalized cells by c-myc. p21 was, however, elevated in the senescent cells and in the cells of crisis. Interestingly, p16 was upregulated whenever T antigen is overexpressed. Telomerase activity was also activated only in the immortalized cells. These results suggest that overexpression of c-myc contributes to immortalization of human diploid fibroblast by activating telomerase activity and suppressing p21 activity.
Asunto(s)
Humanos , Antígenos Transformadores de Poliomavirus/genética , Biomarcadores , Senescencia Celular/genética , Transformación Celular Viral , Células Cultivadas , Ciclinas/metabolismo , Diploidia , Fibroblastos/metabolismo , Genes myc/genética , Inhibidor p16 de la Quinasa Dependiente de Ciclina/metabolismo , Virus 40 de los Simios/genética , Telomerasa/metabolismoRESUMEN
En este artículo describimos la distribución anatómica, las características histológicas y moleculares de 32 casos de LNH. La estadificación clínica y clasificación histológica por grados se hizo de acuerdo a esquemas aceptados convencionalmente. Los arreglos detectados en genes que codifican para Ig o el RcT sirvieron para identificar la estirpe celular y el estadio de diferenciación de las células neoplásicas. El análisis de 26 muestras de suero reveló la existencia de anticuerpos contra epítopes de EBV; ocho de estos pacientes contenían secuencias virales integradas en el genoma del tumor. Nuestros estudios indican que el uso de diferentes métodos es fundamental para profundizar en el conocimiento de la historia natural de los LNH.