RESUMEN
An inhibitor of glucose 6-phosphatase, isosteviol, was used in liver perfusion experiments to obtain a rough estimate of the control strength of the enzyme. Isosteviol only inhibited glucose release at high concentrations (1 mM), well above that needed for half-maximal action (70 micrnM). The decrease in glucose release was followed by an increase in the intracellular glucose 6-phosphate concentration. It was concluded that the control strength of glucose-6-phosphatase is relatively low
Asunto(s)
Ratas , Animales , Diterpenos/farmacología , Hígado/metabolismo , Glucofosfatos/antagonistas & inhibidores , Glucosa/metabolismo , Glucofosfatos/metabolismo , Perfusión , Fosforilasas/metabolismoRESUMEN
A atividade de glicose-6-fosfato desidrogenase em células in vitro foi determinada através de método espectrofotométrico. Foi estabelecida a densidade populacional adequada e padronizaram-se as condiçöes para extraçäo da enzima e para o desenvolvimento da reaçäo