RESUMEN
BACKGROUND: Gastric cancer is a common malignant tumor with high morbidity and mortality worldwide, which seriously affects human health. Gramicidin is a short peptide antibiotic which could be used for treating infection induced by bacteria or fungi. However, the anti-cancer effect of gramicidin on gastric cancer cells and its underlying mechanism remains largely unknown. RESULTS: Gastric cancer cells SGC-7901, BGC-823 and normal gastric mucosal cells GES-1 were treated with different concentrations of gramicidin respectively. The results of CCK-8 experiment revealed cellular toxicity of gramicidin to cancer cells while cell colony formation assay showed that gramicidin significantly inhibited the proliferation of gastric cancer cells, but had little effect on normal gastric mucosal cells. In addition, the wound healing assay showed that gramicidin inhibited the migration of SGC-7901 cell. Meanwhile, apoptosis and cell cycle analysis revealed that gramicidin induced cell apoptosis with G2/M cell cycle inhibition. Furthermore, western blot analysis demonstrated that gramicidin down-regulated the expression of cyclinD1 and Bcl-2 as well as the FoxO1 phosphorylation. CONCLUSIONS: The current study illustrated the anti-tumor activity of gramicidin on gastric cancer cells, providing a possibility for gramicidin to be applied in clinical practice for the treatment of gastric cancer.
Asunto(s)
Humanos , Neoplasias Gástricas/patología , Ciclo Celular/efectos de los fármacos , Movimiento Celular/efectos de los fármacos , Apoptosis/efectos de los fármacos , Proliferación Celular/efectos de los fármacos , Gramicidina/farmacología , Fosforilación , Regulación hacia Abajo , Proteínas Proto-Oncogénicas c-bcl-2/efectos de los fármacos , Proteínas Proto-Oncogénicas c-bcl-2/metabolismo , Ciclina D1/efectos de los fármacos , Ciclina D1/metabolismo , Línea Celular Tumoral , Proteína Forkhead Box O1/efectos de los fármacos , Proteína Forkhead Box O1/metabolismoRESUMEN
A fim de avaliar a sensibilidade "in vitro" à associaçäo de sulfato de framicetina, sulfato de polimixina B e gramicidina, estudamos amostras de 96 pacientes portadores de patologias oculares externas e de 4 lentes de contato gelatinosas. De 112 antibiogramas realizados, 80 (71,4%) foram por Staphylococcus aureus, cuja sensibilidade incluindo todas as bactérias de 85,7% (96). Esta associaçäo é composta por 3 antibióticos bactericidas näo usados sistematicamente de rotina