Validation of a new technique to freezing and embedding tissue in Mohs surgery, using an animal model

ABSTRACT PURPOSE: To validate the innovative Dry Ice method, comparing it with two standard methods currently used for tissue processing in Mohs surgery, the Heat Sink method and the Miami Special. METHODS: Forty eight samples of pigs kin with the standard beveled Mohs technique were used, and randomly allocated into six groups. Each group was processed with one of the 3 methods and evaluated for: The freezing time, the depth required to cut into the block to obtain a complete section, and the quality of histological slides analyzed with a image software. The statistical analysis was performed with the software SAS(r) System. The inferential analysis was made by one-way ANOVA. RESULTS: The Miami Special showed a processing time significantly shorter than Dry Ice method and Heat Sink method. There was no significant difference in the depth required to cut into the blocks, and area of surgical margins visualized. CONCLUSION: The Dry Ice method was as efficient as the other two methods currently used in Mohs surgery, considering the individual advantages and disadvantages of each method.

Distribution and Detection of Severe Fever with Thrombocytopenia Syndrome Virus in Ticks Collected from Jeollanam-do, Korea

Severe fever with thrombocytopenia syndrome (SFTS) is firstly reported in China in 2011. Thereafter it is reported an infectious disease in Japan and Korea. It is caused by bunyavirus, called SFTS virus (SFTSV). The main vector of SFTS is Haemaphysalis longicornis tick. We investigated the distribution and detection of SFTSV in ticks collected from the environment using the dragging method and dry ice fogging method from May to November 2014 in Jeollanam-do, Korea. Sampling was taken from the province Suncheon, Gokseong, Boseong, Goheung where patients have occurred in 2013 and Gurye as control. Among the total 3,048 ticks collected, 3,030 ticks were H. longicornis (99.4%) and 18 were Amblyomma testudinarium. H. longicornis was collected 1,330 ticks in Gokseong, 1,188 ticks in Boseong, 240 ticks in Suncheon, 150 ticks in Goheung and 140 ticks in Gurye. Developmental stages by month of H. longicornis were revealed that nymph (92%) was collected from May to June, adult (30%) and nymph (70%) in July, and 93% of larvae from September to October. These results showed the different dominant stage of ticks according to seasons. However, no SFTSV-specific gene was detected in 3,030 ticks of H. longicornis.

A Case of Carbon Dioxide Poisoning from Dry Ice Inhalation

Carbon dioxide (CO2) poisoning is rare, but its clinical features of acute exposure include cardiovascular, respiratory, neurologic and metabolic aspects. CO2 can also have a direct toxicological effect. At very high concentrations (>9%), CO2 induces respiratory depression and narcotic-like effects on the central nervous system. Especially, acute poisoning from dry ice is caused by CO2 gas inhalation and asphyxiation, and may impose excess load on the myocardium. This case was an acute CO2 poisoning from dry ice gas inhalation. In spite of an initial severe status, the male patient rapidly improved with no complications following the application of highly concentrated oxygen and fluid therapy.

Avaliação da vitalidade pulpar através da aplicação do difluorodihidrocarbono em pacientes submetidos a tratamento ortodôntico / Evaluation of the pulpal vitality through the aplication of the diflorodihidrocarbono in patients submitted to orthodontic treatment

O desconforto ou dor manifestada após a ativação de aparelhos fixos nos transcurso do tratamento ortodôntico justificaram essa investigação no propósito de monitorar a vitalidade pulpar de incisivos centrais superiores por meio de estimulação térmica pelo frio com difluorodihidrocarbono. Foi observada uma redução progressiva da média dos tempos de remissão da sintomatologia dolorosa nas três fases de nivelamento com diferença estatisticamente significante.

Scar Revision with Frostbite Using Dry Ice

Facial scar is one of the most common problems in plastic surgery department. Many various methods are attempted to improve facial scars, but there is no definite satisfactory method on scar revision. Authors have been interested in a tendency to show good results when traumatic lacerations were accompanied by abrasions. The improved appearance of the scars with this situation is the result that there were fewer elevations and depressions, and no break in continuity of the epidermal covering on both sides of the scar. But mechanical or other dermabrasive methods have a risk of hyperpigmentation. Authors suggest the scar revision with frostbite using dry ice. This method has little hyperpigmentation due to the sensitivity of melanocytes to cold injury. And the tissue destruction is progressed selectively because of the resistance of collagen against the cold injury and the preserved dermal fibrous network as the structural framework. These characteristics of cryotherapy improve the result of scar revision with fewer side effects rather than other dermabrasive methods. We performed this method to 349 patients from 1993 to 2003 and obtained high patients' satisfaction (85.9% more than fair). Moreover, this method is simple, cheap and handy. In conclusion, the scar revision with frostbite using dry ice is very useful method to improve facial scars.

The Changes in the Removal Torque of Screws after Local Heating / 대한정형외과연구학회지

PURPOSE: The extraction of well-fixed metallic implants can be extremely demanding and time consuming, resulting in damage surrounding bone. Therefore, a less traumatic method of removing a well-fixed implant from bone is necessary. Our hypothesis was that a well-fixed implant can be extracted more easily if it is heated to a high temperature. The present study was conducted to evaluate the effect of local heating on the removal torque of well-fixed titanium screws. MATERIALS AND METHODS: A titanium screw (2.7 mm diameter, 12 mm length) was bilaterally inserted into the distal femur of 14 New Zealand White rabbits at identical locations. Five rabbits were sacrificed at 8 weeks, and 9 rabbits were sacrificed at 20 weeks after screw insertion. Both femurs were harvested, and screw removal torques were measured using a torque gauge. Before removal, the screws in right femurs were heated with an electric soldering iron and then cooled with dry ice, whereas screws in the left femur were removed without heating. After removing the screws, Hematoxylin & Eosin staining was performed to allow the histological assessment of bone tissues around the implants, and picrosirius red F3BA staining was performed to assess the extent of bone damage. Statistical differences between the heat treated and untreated groups were determined using the Wilcoxon Signed Ranks test and differences with respect to the duration of implantation were evaluated by the Mann-Whitney test. A p value of & .05 was considered to be significant. RESULTS: The removal torques for the heated screws were significantly lower than those for the non-heated screws for both 8 and 20 weeks groups. For non-heated screws, removal torques for the screws implanted 20 weeks was significantly higher than those of the screws implanted for 8 weeks (p & .05). H & E staining showed that the screw-bone interface was denatured in heat treated group. Polarized microscopy after picrosirius red F3BA staining showed that the collagen in the thermally damaged region had denatured, resulting in a loss of its natural birefringence. The maximal depth of the thermally damaged region from the screw/bone interface was less than 400 micrometer. CONCLUSION: Controlled local heating may be a less traumatic method of removing a well-fixed implant from bone.

Hiperplasia epitelial focal: estudio clínico-patológico / Focal epithelial hyperplasia

Niña de 10 años de edad, de raza indo-americana, con placas verrugosas orales de siete meses de evolución. El diagnóstico clínico de hiperplasia epitelial focal fue confirmado por microscopía electrónica. Se realiza la descripción de esta patología frecuente en comunidades indígenas de bajo nivel socioeconómico y su diagnóstico diferencial con otras patologías orales clínicamente similares

Cryosurgery in Becker's Nevus, Nevus Spilus and Congenital Nevocytic Nevus / 대한피부과학회지

BACKGROUND: Cryosurgery for epidermal pigment lesions such as Becker's nevus and nevus spilus could be inexpensive, simple and effective, but has not been published much in detail. OBJECTIVE: We evaluated the clinical efficacy according to its determinants, complications and recurrence rate of the cryosurgery, and compared with those of pigment laser. METHODS: Hard chalk dry ice were applied on the lesions of 17 patients with Becker's nevus, 8 patients with nevus spilus and 6 patients with congenital nevocytic nevus. Two freeze-thaw cycles per lesion were employed, freezing time was chosen arbitarily depending on the characteristics of each lesion. Nineteen patients of the above 31 patients were also treated with PLDL(xenon flashlamp -pumped dye laser: 2.5-3.0J/cm2) or PLTL (Q-Switched alexandrite laser: .5-8.5J/cm2). Accessible patients were evaluated by direct interview or phone. RESULTS: 1. The clinical efficacy of the cryosurgery was better in nevus spilus than in Becker's nevus or congenital nevocytic nevus. 2. In nevus spilus, the clinical efficacy of the cryosurgery was similar to that of pigment laser, while cryosurgery was more effective than laser surgery in Becker's nevus. 3. The most common complication was transient perilesional hyperpigmentation, which could be removed with chemical peeling or dermabrasion. CONCLUSION: Cryosurgery for epidermal pigment lesions was an inexpensive, simple and effective procedure. In some cases, cryosurgery showed better results than pigment laser, and its efficacy may be improved by adjunctive laser treatment. The rate of recurrence after cryosurgery was lower than that of laser surgery. Cryosurgery would be recommendable for treating epidermal pigment lesions.

Confiabilidade do gelo e do tetrafluoroetano na determinaçäo da vitalidade pulpar / Reliability of ice and tetrafluoroethane for determining dental pulp vitality

Objetivou-se avaliar a confiabilidade do bastäo de gelo e do gás refrigerante tetrafluoroetano na detecçäo da vitalidade pulpar em 2420 dentes humanos íntegros de 148 pacientes de ambos os sexos, com idade variável entre 20 e 30 anos. Pôde-se concluir que o tetrafluoroetano, em relaçäo ao bastäo de gelo, produziu índice de acerto maior e estatisticamente significante (p < 0,01) na determinaçäo da vitalidade pulpar em todos os grupos de dentes testados. Relativamente ao tetrafluoroetano, foi o seguinte o grau de confiabilidade em ordem decrescente: incisivos inferiores, caninos inferiores, pré molares superiores, pré-molares inferiores, molares superiores, incisivos superiores, caninos superiores, molares inferiores. Com respeito ao bastäo de gelo, o grau de confiabilidade, em ordem decrescente, assim se portou: incisivos inferiores, incisivos superiores, pré-molares superiores, caninos superiores, caninos inferiores, pré-molares inferiores, molares superiores, molares inferiores

A Case of Ota's Nevus Treated by Combination Therapy with Cryosurgery and the Q-Switched Alexandrit Laser / 대한피부과학회지

Ota's nevus is a mongolian spot-like macular blue-black or gray-brown patchy pigmentation that most commonly occurs unilaterally in areas innervated by the first and second division of the trigeminal nerve. We report a case of Ota's nevus treated by combination therapy with cryosurgery and a Q-switched Alexandrite laser. A 48-year-old female had had a type III Ota's nevus from birth. Cryosurgery was tried at first using hard chalk dry ice on the lesion of the forehead and cheek. After that, The Q-switched Alexandrite laser was used three times at twelve week intervals at the site of the eyelid. Good therapeutic effects were gained and our patient was very satisfied. There were no complications or scarring. A Biopsy of the area treated revealed a similar histological pattern with significantly less melanin indicating melanin removal. Combination therapy with cryosurgery and an Alexandrite laser on the Ota's nevus may be cheaper, more effective and a safer method. It may also reduce the limitation of the two methods when used individually.

Eficácia do bastäo de gelo e do tetrafluoroetano na determinaçäo da vitalidade pulpar / Effectiveness of ice and tetrafluoroethane in detecting pulpal vitality

O presente trabalho teve o objetivo de avaliar a eficácia do bastäo de gelo e do tetrafluoroetano na determinaçäo da vitalidade pulpar em 594 dentes humanos cariados, restaurados e integros de 72 pacientes, com idade variável entre 47 a 60 anos. Verificou-se que a aplicaçäo do gás de tetrafluoroetano propiciou maior índice de acerto quando comparado com o bastäo de gelo, havendo diferença estatisticamente significante entre os dois agentes térmicos utilizados (p<0,01)

Estudo comparativo da eficiência do gelo e da neve carbônica na determinaçäo da vitalidade pulpar em dentes permanentes portadores de rizogênese incompleta / The comparative study of the ice pencil and snow pencil efficacy to determine the pulp vitality in young permanent teeth

Os autores abordam a problemática em se determinar vitalidade pulpar em dentes permanentes jovens com rizogênese incompleta. A literatura relaciona inervaçäo imatura e maior amplitude do forame apical como sendo os fatores mais implicados nesta questäo. Para tal os autores compararam a efetividade de dois agentes térmicos bastante empregados na determinaçäo de vitalidade pulpar

A determinaçäo da vitalidade pulpar em dentes decíduos através da utilizaçäo do gelo e da neve carbônica / Pulpar vitality determination in deciduous teeth using ice and snow pencil

O estabelecimento de um correto diagnóstico pulpar, em Odontopediatria, constitui-se em uma tarefa difícil na prática diária. Tal dificuldade está relacionada com as peculiaridades do paciente infantil, bem com as características próprias do dente decíduo. Buscando um maior esclarecimento neste assunto, este estudo tem como objetivo testar o grau de resposta destes dentes, quando submetidos à aplicaçäo de testes de vitalidade pulpar (gelo e neve carbônica)

Clinical Evaluations of Cryosurgery for Nevus of Ota / 대한피부과학회지

BACKGROUND: Cryosurgical treatment for nevus of Ota could be effective and cheap, but has not been introduced too much in detail. OBJECTIVE: This study was done to evaluate the clinical efficacy according to its determinants, and the complications of cryosurgery. METHODS: 109 patients with nevus of Ota were treated with hard chalk dry ice which contacted the skin consistently of was rolled fast. Accessible patients were evaluated by phone or direct interview. RESULTS: Satisfactions assessed by patients after cryosurgery were excellent 21%, moderate 51%, fair 17% and poor 11%, respectively. Satisfactions assessed by doctor were quite higher than those by patient. Satisfactions assessed by patients were affected by clinical determinants such as age over 40 and color of the lesion, but not by clinical type, number of sessions, and combinations with laser. Complications were in the following order : textural change, hypopigmentation, hypertrophic scar, persistent erythema, hyperpigmentation, infection and milia formation. CONCLUSION: Cryosurgery using dry ice for the treatment of nevus of Ota was quite effective but technical improvements for decreasing complications or combinations with Q switched laser and other surgical techniques might be required.

Distribution of Thiol-specific Antioxidant Protein Immunoreactivity in the Mammalian Central Nervous Systern

Thiol-specific antioxidant protein (TSA) is the antioxidant protein which specifically inhibits the inactivation of various enzymes by a nonenzymatic mixedfunction oxidation (MFO) system containing a sulfhydryl compound as reducing equivalent but not by the MFO system containing a nonsulf hydryl reducing equivalent. TSA was isolated and purified from Saccharomyces cerevisiae and bovine brain. But localization in the brain and physiological role of TSA as an antioxidant enzyme a-re known very little. The localization of TSA protein in the rat brain and rabbit spinal cord was examined with polygonal antibodies to bovine TSA made in rabbit. Tissues were fixed with 4% paraformaldehyde, frozen in dry ice, sectioned on a sliding microtome, incubated with these antibodies, and then processed for avidin-biotin peroxidase complex staining. The irrimunoreactive (IR) cellular element for TSA in the central nervous system - ne-om The IR product for TSA was mainly located m neuronal soma and proximal part of neuronal process such as apical dendnte of pyranudal cell of the cerebral cortex. The glial cell, blood vessel and nucleus of neuron did not show the TSA IR TSA IR neurons were found at every nucleus and cortex mcluding cerebral cortex, hippocampus, corpus striatum, cerebellar cortex, thalamus, septum and spinal gray matter. In hypoxia rabbit spinal cord, there were dense and light IR neurons, and the former was considered to be miured by hypoxic msult These results indicate that TSA is ubiquitous protem in neurons of mammalian central nervous system and show uneven distribution among individual neurons in same nucleus and different nucleus. And TSA may be induced by increased oxidative pressure after ischemia.

Avaliaçäo in vitro dos efeitos na superfície do esmalte dentário humano utilizando-se bastäo de neve carbônica / An in vitro evaluation of the effects on the surface of human dental enamel utilizing carbon dioxide snow

O presente estudo avaliou os efeitos danosos sobre a superfície do esmalte dentário humano frente ao emprego do bastäo de neve carbônica. Tanto à luz de microscopia ótica quanto ao emprego do corante Penetrant P-149, näo se observou nenhuma rachadura em nível de esmalte após a aplicaçäo do bastäo de neve carbônica durante 10 segundos

Consideraçöes em torno do emprego do bastäo de neve carbônica, na determinaçäo da vitalidade pulpar. Parte III: possíveis danos às estruturas do esmalte e polpa dentários / Considerations about the use of CO2 snow pencil in the determination of pulp vitality

Os autores, com base na revista da literatura, discutem acerca do emprego do bastäo de neve carbônica, na determinaçäo da vitalidade pulpar, e mostram que a ocorrência de possíveis rachaduras no esmalte dentário decorrentes da aplicaçäo do bastäo de neve carbônica neste sítio resulta, muito provavelmente, da exteriorizaçäo de rachaduras internas pré-existentes neste tecido, e que o bastäo de neve carbônica, quando aplicado sob a superfície da coroa dentária, näo promove danos ao conjuntivo pulpar

Avaliaçäo "in vitro" dos possíveis efeitos danosos na superfície do esmalte dentário humano frente ao emprego do bastäo de neve carbônico / Evaluation \"in vitro\" of the possible noxious effects in the surface of human enamel after the aplication of carbon dioxide

O presente estudo avaliou os efeitos danosos sobre a superfície do esmalte humano frente ao emprego do bastäo de neve carbônica, concluindo-se que: 1) as periquimácias observadas, à luz da microscopia ótica, na superfície do esmalte dentário dos dentes testados, näo se alteraram após à aplicaçäo do bastäo de neve carbônica. 2) frente à aplicaçäo do bastäo de neve carbônica, näo foi observada a formaçäo de rachaduras na superfície do esmalte dentário dos dentes testados, tanto ao exame pela microscopia ótica como ao emprego do corante fluorescente penetrante evidenciado pela luz ultra-violeta

Consideraçöes em torno do emprego do bastäo de neve carbônica na determinaçäo da vitalidade pulpar. Parte II: capacidade refrigerante / Concerning the use of carbonic snow pencil in the dental pulp vitality determination. Part II. Capacity of the refrigeration

Com base na revista da literatura, discute-se acerca do emprego do bastäo de neve carbônica, na determinaçäo da vitalidade pulpar e atribui-se que, graças à sua baixa temperatura, ocasiona rápido e significativo decréscimo da temperatura intrapulpar, quando aplicado sobre a superfície da coroa dentária

Consideraçöes em torno do emprego do bastäo de neve carbônica na determinaçäo da vitalidade pulpar. Parte I: grau de confiabilidade / Considerations about the CO2 snow pencil as a dental pulp vitality test. Part I: confiability

Com o auxílio da revista da literatura, discute-se acerca do emprego do bastäo de neve carbônica, na determinaçäo da vitalidade pulpar, e conclui-se que constitui método que se reveste de alto grau de confiabilidade