Effects of metoclopramide on the expression of metalloproteinases and interleukins in left colonic anastomoses. An experimental study

PURPOSE : To evaluate the effects of metoclopramide on metalloproteinases (MMP) and interleukins (IL) gene expression in colonic anastomoses in rats. METHODS : Eighty rats were divided into two groups for euthanasia on the 3rd or 7th postoperative day (POD), then into two subgroups for sepsis induction or not, and then into subgroups to receive either metoclopramide or saline solution. Left colonic anastomosis were performed and then analyzed. RESULTS : On the 3rd POD, metoclopramide was associated with increased expression of MMP-1a, MMP-13, and TNF-α. On the 7th POD, the transcripts of all MMPs, TNF-α, IL-1β, IFN-γ, and IL-10 of the treated animals became negatively modulated. In the presence of sepsis, metoclopramide did not change MMPs and decreased IL-6, IL-1β, IFN-γ and IL-10 gene expression on the 3rd POD. On the 7th POD, increased expression of all MMPs, IFN-γ and IL-10 and negative modulated TNF-α and IL-6 gene expression. CONCLUSION : Administration of metoclopramide increased metalloproteinases and interleukins gene expression on the 3rd postoperative day and negatively modulated them on the 7th POD. In the presence of abdominal sepsis, metoclopramide did not change MMPs and decreased ILs gene expression on the 3rd POD. On the 7th POD, the drug increased expression of all MMPs.

Medición de la presión intraabdominal para la vigilancia posoperatoria de la cirugía abdominal / Measurement of intraabdominal pressure for the postoperative surveillance of abdominal surgery

Se realizó un estudio de serie de casos de 286 pacientes laparotomizados que ingresaron en la Unidad de Cuidados Intensivos del Hospital General Vladimir Ilich Lenin (Holguín), entre el 4 de marzo de 1999 y el 29 de enero del 2003. El objetivo fue describir el comportamiento de la edad, sexo, diagnóstico al ingreso, frecuencia de complicaciones intraabdominales posoperatorias y el resultado al egreso de estos pacientes. Según el APACHE II, la escala pronóstica de gravedad fue de 13,1. Las principales causas del ingreso fueron peritonitis (34,9 por ciento) y politraumatismos (19,52 por ciento). Encontramos complicaciones posoperatorias en el 32,5 por ciento de los pacientes. La presión intraabdominal en los pacientes complicados fue de 14,94 ± 4,88 cm H2O, mientras que en los pacientes que no tuvieron complicaciones fue de 11,67 ± 4,63 cm H2O (F = 31,4948; p < 0,05). Hubo 94 fallecidos (32,8 por ciento) y su presión intraabdominal fue de 14,22 ± 5,18 cm H2O. En cambio, en los sobrevivientes la presión fue de 12,07 ± 4,74 cm H2O (F = 12,2824; p < 0,05). Los pacientes con complicaciones posoperatorias y con resultados desfavorables al egreso de la unidad de cuidados intensivos tienen una presión intraabdominal más alta que los que evolucionan favorablemente(AU)

A case series study that included 286 laparotomized patients that were admitted at the ICU of Vladimir Ilich Lenin General Hospital, in Holguin, between March 4, 1999 and January 29, 2003, was conducted. The objective was to describe the behavior of age, sex, diagnosis on admission, frequency of intraabdominal postoperative complications, and the result attained in these patients on discharge. According to APACHE II, the prognostic scale of severity was 13.1. The main cause of hospitalization were peritonitis (34.9 per cent) and polytraumas (19.52 per cent). Postoperative complications were found in 32.5 per cent of the patients. Intraabdominal pressure in the complicated patients was 14.94 ± 4.88 cm H2O; whereas, in those patients without complications it was11.67 ± 4.63 cm H2O (F = 31.4948; p < 0.05). There were 94 deaths (32.8 per cent) and their intrabdominal pressure was 14.22 ± 5.18 cm H2O. However, the survivors’ pressure was 12.07 ± 4.74 cm H2O (F = 12.2824; p < 0.05). The patients with postoperative complications and unfavorable results on discharge from the ICU had a higher intrabdominal pressure than those with a satisfactory evolution(AU)