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1.
Rev. chil. infectol ; 38(5): 613-621, oct. 2021. ilus, tab
Artículo en Español | LILACS | ID: biblio-1388288

RESUMEN

INTRODUCCIÓN: Elizabethkingia es un género de bacterias gramnegativas cuya relevancia como patógeno oportunista en hospederos inmunocomprometidos y pacientes críticos ha sido reconocida progresivamente en los últimos años. Este género está compuesto principalmente por E. meningoseptica, E. anophelis y E. miricola. Si bien inicialmente E. meningoseptica fue considerada la especie patógena más relevante, gracias a los avances en las técnicas de identificación microbiológica se ha reconocido a E. anophelis como el principal patógeno de este grupo. OBJETIVO: Caracterizar los casos de infecciones por Elizabethkingia spp. en una red de salud y realizar una breve revisión de esta infección. MATERIAL Y MÉTODOS: Se realizó una revisión de los cultivos positivos para Elizabethkingia spp. en el Laboratorio de Microbiología de la Red de Salud UC-CHRISTUS (Chile) entre los años 2017 y 2021. RESULTADOS: Se obtuvo 17 cultivos positivos correspondientes a siete casos clínicos, todos procedentes de un hospital universitario. Todos los casos poseían factores de riesgo conocidos de infección por Elizabethkingia spp. incluyendo uso de antimicrobianos recientes, por ejemplo, el uso previo de carbapenémicos en 85,7% de los pacientes. Cuatro casos se presentaron en pacientes con neumonía por SARS-CoV-2, una coinfección no previamente reportada en la literatura. Elizabethkingia anophelis fue identificada mediante secuenciación de ARN ribosomal en 80% de las cepas recuperadas, lo que corresponde al primer reporte de esta especie en Chile CONCLUSIÓN: Comunicamos la experiencia clínica de infecciones por este género en un hospital universitario de Chile, incluyendo los primeros casos de coinfección en pacientes cursando neumonía por SARS-CoV-2 y la primera identificación de Elizabethkingia anophelis en Chile.


BACKGROUND: Elizabethkingia is a genus of gramnegative bacteria whose relevance as an opportunistic pathogen in immunosuppressed hosts and critically ill patients has been progressively recognized in recent years. This genus is mainly composed of E. meningoseptica, E. anophelis, and E. miricola. Although E. meningoseptica was initially reported as the most relevant pathogenic species, thanks to advances in microbiological identificaron techniques E. anophelis has been recognized as the main pathogen of this group. AIM: To characterize Elizabethkingia spp.'s infections in a health network and make a brief review of this infection. METHOD: We conducted a review of clinical cultures that were positive for Elizabethkingia sp. in the Microbiology Laboratory of the UC-CHRISTUS Health Network (Chile), between 2017 and 2021. RESULTS: Seventeen positive cultures were obtained corresponding to seven clinical cases, all originating from a university hospital. All cases had known risk factors for Elizabethkingia sp. infection, including recent use of antibiotics. Notably, previous use of carbapenems was present in 85.7% of the patients. Four cases occurred in patients with SARS-CoV-2 pneumonia, a coinfection not previously reported in the literature. Elizabethkingia anophelis was identified by ribosomal RNA sequencing in 80% of the recovered strains, which corresponds to the first report of this species in Chile. CONCLUSION: We report the clinical experience of a university hospital with infections by Elizabethkingia spp., including the first cases of coinfection in patients with SARS-CoV-2 pneumonía and the first identification of Elizabethkingia anophelis in Chile.


Asunto(s)
Humanos , Masculino , Femenino , Persona de Mediana Edad , Anciano , Infecciones por Flavobacteriaceae/microbiología , Infecciones por Flavobacteriaceae/epidemiología , Coinfección/epidemiología , COVID-19/epidemiología , Chile/epidemiología , Flavobacteriaceae , SARS-CoV-2 , Hospitales Universitarios
2.
Rev. chil. infectol ; 38(2): 297-299, abr. 2021. ilus
Artículo en Español | LILACS | ID: biblio-1388238

RESUMEN

Resumen Las infecciones por bacterias gramnegativas del género Myroides son muy poco frecuentes y generalmente afectan la piel y tejidos blandos de pacientes con algún grado de inmunocompromiso. Presentamos un caso de una mujer de 23 años, con antecedentes de mielomeningocele operado y pie bot, que cursó con una infección profunda de la extremidad inferior derecha por Myroides odoratimimus. La identificación de especie se realizó con técnica de MALDI-TOF. El tratamiento fue inicialmente con meropenem y ajustado a ciprofloxacina, junto con realizar una amputación supramaleolar derecha.


Abstract Infections due to Gram-negative bacteria of the genus Myroides are very rare and generally affect the skin and soft tissues of patients with some degree of immunocompromise. We present a case of a 23-year-old patient with a history of myelomeningocele surgically resolved at 3 years of age and bot foot, who presented with a deep infection of the right lower extremity by Myroides odoratimimus. The species identification was carried out with MALDI-TOF and the treatment was initially carried out with meropenem and finally then ciprofloxacin, in addition to right supramaleolar amputation.


Asunto(s)
Humanos , Femenino , Adulto , Adulto Joven , Osteomielitis/tratamiento farmacológico , Infecciones de los Tejidos Blandos/tratamiento farmacológico , Infecciones por Flavobacteriaceae/diagnóstico , Infecciones por Flavobacteriaceae/tratamiento farmacológico , Farmacorresistencia Bacteriana , Flavobacteriaceae
3.
Rev. peru. med. exp. salud publica ; 35(2): 326-332, abr.-jun. 2018. tab, graf
Artículo en Español | LILACS | ID: biblio-961870

RESUMEN

RESUMEN Las infecciones causadas por microorganismos poco comunes son objeto de investigación, ya que animar a los investigadores a encontrar las medidas sanitarias necesarias para prevenir y tratar la enfermedad, así como la búsqueda de nuevas luces sobre las interacciones humano-microbios. En este informe se describe el caso de un recién nacido varón diagnosticado de hidrocefalia y mielomeningocele, que desarrolló ventriculitis y sepsis por Empedobacter brevis resistente. Este caso pone de manifiesto la inesperada identificación de esta bacteria en el líquido cefalorraquídeo y su patrón multirresistente, que fue crucial para dar un manejo terapéutico adecuado. Esta bacteria evidencia una mezcla de diferentes etiologías en el análisis del líquido cefalorraquídeo.


ABSTRACT Infections caused by rare micro-organisms are the subject of research, as researchers are encouraged to find the necessary health measures to prevent and treat the disease, as well as the search for new insights into human-microbial interactions. This report describes the case of a newborn boy diagnosed with hydrocephalus and myelomeningocele who developed ventriculitis and sepsis from resistant Empedobacter brevis. This case highlights the unexpected identification of this bacterium in the cerebrospinal fluid and its multi-resistant pattern, which was crucial for proper therapeutic management. This bacterium shows a mixture of different etiologies in the analysis of cerebrospinal fluid.


Asunto(s)
Humanos , Recién Nacido , Masculino , Flavobacterium , Infecciones por Flavobacteriaceae , Ventriculitis Cerebral/microbiología , Perú , Infecciones por Flavobacteriaceae/diagnóstico , Infecciones por Flavobacteriaceae/tratamiento farmacológico , Ventriculitis Cerebral/diagnóstico , Ventriculitis Cerebral/tratamiento farmacológico
4.
Biomedical and Environmental Sciences ; (12): 403-406, 2018.
Artículo en Inglés | WPRIM | ID: wpr-690642

RESUMEN

Seasonal outbreaks of airsacculitis in China's poultry cause great economic losses annually. This study tried to unveil the potential role of Avian metapneumovirus (AMPV), Ornithobacterium rhinotracheale (ORT) and Chlamydia psittaci (CPS) in avian airsacculitis. A serological investigation of 673 breeder chickens and a case-controlled study of 430 birds were undertaken. Results showed that infection with AMPV, ORT, and CPS was highly associated with the disease. The correlation between AMPV and CPS were positively robust in both layers and broilers. Finally, we determined the co-infection with AMPV, ORT, and CPS was prevalent in the sampled poultry farms suffering from respiratory diseases and the outbreak of airsacculitis was closely related to simultaneous exposure to all three agents.


Asunto(s)
Animales , Humanos , Sacos Aéreos , Microbiología , Patología , Anticuerpos Antibacterianos , Sangre , Anticuerpos Antivirales , Sangre , Estudios de Casos y Controles , Pollos , Chlamydia , Infecciones por Chlamydia , Microbiología , Patología , Coinfección , Infecciones por Flavobacteriaceae , Microbiología , Patología , Metapneumovirus , Ornithobacterium , Infecciones por Paramyxoviridae , Patología , Virología , Enfermedades de las Aves de Corral , Microbiología , Patología , Virología , Enfermedades Respiratorias , Microbiología , Virología , Estudios Seroepidemiológicos
7.
Braz. j. microbiol ; 46(4): 969-976, Oct.-Dec. 2015. tab, graf
Artículo en Inglés | LILACS | ID: lil-769656

RESUMEN

Abstract Yellow pigmented, filamentous, Gram-negative bacteria belonging to genus Flavobacterium are commonly associated with infections in stressed fish. In this study, inter-species diversity of Flavobacterium was studied in apparently healthy freshwater farmed fishes. For this, ninety one yellow pigmented bacteria were isolated from skin and gill samples (n = 38) of three farmed fish species i.e. Labeo rohita, Catla catla and Cyprinus carpio. Among them, only twelve bacterial isolates (13.18%) were identified as Flavobacterium spp. on the basis of morphological, biochemical tests, partial 16S rDNA gene sequencing and phylogenetic analysis. On the basis of 16S rDNA gene sequencing, all the 12 isolates were 97.6-100% similar to six different formally described species of genus Flavobacterium. The 16S rDNA based phylogenetic analysis grouped these strains into six different clades. Of the 12 isolates, six strains (Fl9S1-6) grouped with F. suncheonense, two strains (Fl6I2, Fl6I3) with F. indicum and the rest four strains (Fl1A1, Fl2G1, Fl3H1 and Fl10T1) clustered with F. aquaticum, F. granuli, F. hercynium and F. terrae, respectively. None of these species except, F. hercynium were previously reported from fish. All the isolated Flavobacterium species possessed the ability of adhesion and biofilm formation to colonize the external surface of healthy fish. The present study is the first record of tropical freshwater farmed fishes as hosts to five environmentally associated species of the Flavobacterium.


Asunto(s)
Animales/clasificación , Animales/genética , Animales/aislamiento & purificación , Animales/microbiología , Animales/fisiología , Animales/veterinaria , ADN Bacteriano/clasificación , ADN Bacteriano/genética , ADN Bacteriano/aislamiento & purificación , ADN Bacteriano/microbiología , ADN Bacteriano/fisiología , ADN Bacteriano/veterinaria , ADN Ribosómico/clasificación , ADN Ribosómico/genética , ADN Ribosómico/aislamiento & purificación , ADN Ribosómico/microbiología , ADN Ribosómico/fisiología , ADN Ribosómico/veterinaria , Enfermedades de los Peces/clasificación , Enfermedades de los Peces/genética , Enfermedades de los Peces/aislamiento & purificación , Enfermedades de los Peces/microbiología , Enfermedades de los Peces/fisiología , Enfermedades de los Peces/veterinaria , Peces/clasificación , Peces/genética , Peces/aislamiento & purificación , Peces/microbiología , Peces/fisiología , Peces/veterinaria , Infecciones por Flavobacteriaceae/clasificación , Infecciones por Flavobacteriaceae/genética , Infecciones por Flavobacteriaceae/aislamiento & purificación , Infecciones por Flavobacteriaceae/microbiología , Infecciones por Flavobacteriaceae/fisiología , Infecciones por Flavobacteriaceae/veterinaria , Flavobacterium/clasificación , Flavobacterium/genética , Flavobacterium/aislamiento & purificación , Flavobacterium/microbiología , Flavobacterium/fisiología , Flavobacterium/veterinaria , Agua Dulce/clasificación , Agua Dulce/genética , Agua Dulce/aislamiento & purificación , Agua Dulce/microbiología , Agua Dulce/fisiología , Agua Dulce/veterinaria , India/clasificación , India/genética , India/aislamiento & purificación , India/microbiología , India/fisiología , India/veterinaria , Datos de Secuencia Molecular/clasificación , Datos de Secuencia Molecular/genética , Datos de Secuencia Molecular/aislamiento & purificación , Datos de Secuencia Molecular/microbiología , Datos de Secuencia Molecular/fisiología , Datos de Secuencia Molecular/veterinaria , Filogenia/clasificación , Filogenia/genética , Filogenia/aislamiento & purificación , Filogenia/microbiología , Filogenia/fisiología , Filogenia/veterinaria , /clasificación , /genética , /aislamiento & purificación , /microbiología , /fisiología , /veterinaria
9.
Korean Journal of Ophthalmology ; : 133-136, 2013.
Artículo en Inglés | WPRIM | ID: wpr-143902

RESUMEN

To report keratitis with Elizabethkingia meningoseptica, which occurred in a healthy patient after wearing contact lenses for 6 months. A 24-year-old male patient visited our hospital with ocular pain. This patient had a history of wearing soft contact lenses for 6 months, about 10 hours per day. At initial presentation, slit lamp examination showed corneal stromal infiltrations and small epithelial defect. Microbiological examinations were performed from corneal scrapings, contact lenses, and the contact lens case and solution. The culture results from contact lenses, contact lens case and solution were all positive for Elizabethkingia meningoseptica. Thus, we could confirm that the direct cause of keratitis was contamination of the contact lenses. The patient was treated with 0.3% gatifloxacin. After treatment, the corneal epithelial defect was completely healed, and a slight residual subepithelial corneal opacity was observed. We diagnosed keratitis with Elizabethkingia meningoseptica in a healthy young male wearing soft contact lenses. We conclude that Elizabethkingia meningoseptica should be considered as a rare but potential pathogen for lens-related keratitis in a healthy host.


Asunto(s)
Humanos , Masculino , Adulto Joven , Chryseobacterium , Lentes de Contacto Hidrofílicos/efectos adversos , Infecciones por Flavobacteriaceae/complicaciones , Queratitis/etiología
10.
Korean Journal of Ophthalmology ; : 133-136, 2013.
Artículo en Inglés | WPRIM | ID: wpr-143895

RESUMEN

To report keratitis with Elizabethkingia meningoseptica, which occurred in a healthy patient after wearing contact lenses for 6 months. A 24-year-old male patient visited our hospital with ocular pain. This patient had a history of wearing soft contact lenses for 6 months, about 10 hours per day. At initial presentation, slit lamp examination showed corneal stromal infiltrations and small epithelial defect. Microbiological examinations were performed from corneal scrapings, contact lenses, and the contact lens case and solution. The culture results from contact lenses, contact lens case and solution were all positive for Elizabethkingia meningoseptica. Thus, we could confirm that the direct cause of keratitis was contamination of the contact lenses. The patient was treated with 0.3% gatifloxacin. After treatment, the corneal epithelial defect was completely healed, and a slight residual subepithelial corneal opacity was observed. We diagnosed keratitis with Elizabethkingia meningoseptica in a healthy young male wearing soft contact lenses. We conclude that Elizabethkingia meningoseptica should be considered as a rare but potential pathogen for lens-related keratitis in a healthy host.


Asunto(s)
Humanos , Masculino , Adulto Joven , Chryseobacterium , Lentes de Contacto Hidrofílicos/efectos adversos , Infecciones por Flavobacteriaceae/complicaciones , Queratitis/etiología
11.
Braz. j. infect. dis ; 16(4): 390-392, July-Aug. 2012. tab
Artículo en Inglés | LILACS | ID: lil-645430

RESUMEN

Members of the genus Myroides are aerobic Gram-negative bacteria that are common in environmental sources, but are not components of the normal human microflora. Myroides organisms behave as low-grade opportunistic pathogens, causing infections in severely immunocompromised patients and rarely, in immunocompetent hosts. A case of Myroides odoratimimus cellulitis following a pig bite in an immunocompetent child is presented, and the medical literature on Myroides spp. soft tissue infections is reviewed.


Asunto(s)
Adolescente , Animales , Humanos , Masculino , Mordeduras y Picaduras/complicaciones , Celulitis (Flemón)/microbiología , Infecciones por Flavobacteriaceae/microbiología , Flavobacteriaceae/aislamiento & purificación , Infecciones de los Tejidos Blandos/microbiología , Celulitis (Flemón)/diagnóstico , Infecciones por Flavobacteriaceae/diagnóstico , Inmunocompetencia , Porcinos , Infecciones de los Tejidos Blandos/diagnóstico
12.
Indian J Med Microbiol ; 2012 Jul-Sept; 30(3): 370-372
Artículo en Inglés | IMSEAR | ID: sea-143991

RESUMEN

Chryseobacterium species is an uncommon human pathogen although recovered from various sources in the hospital environment. Most infections have been detected in hospitalized patients with severe underlying diseases and who had indwelling devices or implants. Despite their low virulence, chryseobacteria are inherently resistant to many antimicrobial agents. We report a rare case of urinary tract infection by Chryseobacterium indologenes in a young girl, operated for renal calculus and successfully treated with piperacillin-tazobactam combination.


Asunto(s)
Antibacterianos/administración & dosificación , Chryseobacterium/aislamiento & purificación , Femenino , Infecciones por Flavobacteriaceae/diagnóstico , Infecciones por Flavobacteriaceae/microbiología , Infecciones por Flavobacteriaceae/patología , Humanos , Cálculos Renales/cirugía , Ácido Penicilánico/administración & dosificación , Ácido Penicilánico/análogos & derivados , Piperacilina/administración & dosificación , Complicaciones Posoperatorias/diagnóstico , Complicaciones Posoperatorias/microbiología , Complicaciones Posoperatorias/patología , Resultado del Tratamiento , Infecciones Urinarias/diagnóstico , Infecciones Urinarias/microbiología , Infecciones Urinarias/patología , Adulto Joven
14.
Braz. j. microbiol ; 42(1): 282-289, Jan.-Mar. 2011. tab
Artículo en Inglés | LILACS | ID: lil-571401

RESUMEN

Columnaris disease is one of the main causes of mortality in tilapia rearing and is responsible for large economic losses worldwide. Hematology is a tool that makes it possible to study organisms' physiological responses to pathogens. It may assist in making diagnoses and prognoses on diseases in fish populations. The hematological variables of nile tilapia were studied in specimens with a clinical diagnosis of columnaris disease and in specimens that were disease-free. The total erythrocyte count, hemoglobin rate, hematocrit percentage, mean corpuscular hemoglobin (MCH), mean corpuscular hemoglobin concentration (MCHC), mean corpuscular volume (MCV), organic defense blood cell percentages (leukocytes and thrombocytes) and hepatosomatic and splenosomatic index were determined. The results showed that there were changes in the erythrocytic series and in organic defense blood cells, in the fish infected with the bacterium, with reductions in erythrocytic variables and significant increases in the numbers of circulating lymphocytes and neutrophils.


Asunto(s)
Animales , Recuento de Células Sanguíneas , Cíclidos , Infecciones por Flavobacteriaceae , Flavobacterium , Hematología/métodos , Técnicas y Procedimientos Diagnósticos , Peces , Hematócrito , Métodos
15.
Biomedical and Environmental Sciences ; (12): 694-696, 2011.
Artículo en Inglés | WPRIM | ID: wpr-235579

RESUMEN

A strain of Flavobacterium lindanitolerans isolated from a sick child's ascites was described. The 16S rRNA gene of the strain was 100% identical to that of Flavobacterium lindanitolerans which was first identified in India in 2008. It was first described that the isolate required X factor (Hemin) for growth in the optimal conditions of 37 °C with 5% CO(2). The isolate produced indole and H(2)S. It did not present hemolytic feature on blood agar.


Asunto(s)
Preescolar , Humanos , Líquido Ascítico , Microbiología , Enterovirus Humano A , Infecciones por Enterovirus , Microbiología , Virología , Resultado Fatal , Infecciones por Flavobacteriaceae , Microbiología , Virología , Flavobacterium , Clasificación , Genética , ARN Ribosómico 16S , Genética , Reacción en Cadena de la Polimerasa de Transcriptasa Inversa
16.
Journal of Veterinary Science ; : 85-87, 2010.
Artículo en Inglés | WPRIM | ID: wpr-160868

RESUMEN

In order to evaluate the genetic variability of the causative agent of cold water disease (CWD), plasmid profiling was used to characterize Flavobacterium (F.) psychrophilum isolates (n = 169). Size analysis of plasmids in F. psychrophilum isolates (n = 128) from several fish species demonstrated that six kinds of plasmids were harbored, and ayu isolates had different profiles compared to other isolates. Moreover, multiple isolates (n = 41) from CWD outbreaks in 2002 to 2003 at a single ayu farm were examined to determine differences between isolates from successive outbreaks and showed different profiles by the sources of seedlings.


Asunto(s)
Animales , ADN Bacteriano/genética , Brotes de Enfermedades/veterinaria , Electroforesis en Gel de Agar/veterinaria , Enfermedades de los Peces/genética , Infecciones por Flavobacteriaceae/microbiología , Flavobacterium/genética , Variación Genética/genética , Japón , Osmeriformes , Plásmidos/genética
17.
Braz. j. biol ; 68(2): 409-414, May 2008. ilus, tab
Artículo en Inglés | LILACS | ID: lil-486769

RESUMEN

Flavobacterium columnare is the causative agent of columnaris disease in freshwater fish, implicated in skin and gill disease, often causing high mortality. The aim of this study was the isolation and characterization of Flavobacterium columnare in tropical fish in Brazil. Piracanjuba (Brycon orbignyanus), pacu (Piaractus mesopotamicus), tambaqui (Colossoma macropomum) and cascudo (Hypostomus plecostomus) were examined for external lesions showing signs of colunmaris disease such as greyish white spots, especially on the head, dorsal part and caudal fin of the fish. The sampling comprised 50 samples representing four different fish species selected for study. Samples for culture were obtained by skin and kidney scrapes with a sterile cotton swabs of columnaris disease fish and streaked onto Carlson and Pacha (1968) artificial culture medium (broth and solid) which were used for isolation. The strains in the liquid medium were Gram negative, long, filamentous, exhibited flexing movements (gliding motility), contained a large number of long slender bacteria and gathered into ‘columns'. Strains on the agar produced yellow-pale colonies, rather small, flat that had rhizoid edges. A total of four Flavobacterium columnare were isolated: 01 Brycon orbignyanus strain, 01 Piaractus mesopotamicus strain, 01 Colossoma macropomum strain, and 01 Hypostomus plecostomus strain. Biochemical characterization, with its absorption of Congo red dye, production of flexirubin-type pigments, H2S production and reduction of nitrates proved that the isolate could be classified as Flavobacterium columnare.


Flavobacterium columnare é o agente etiológico da columnariose em peixes de água doce, ocasionando enfermidade na pele e nas brânquias, provocando freqüentemente um grande número de mortalidade. O objetivo deste estudo foi o isolamento e a caracterização de Flavobacterium columnare em peixes tropicais no Brasil. Piracanjuba (Brycon orbignyanus), pacu (Piaractus mesopotamicus), tambaqui (Colossoma macropomum) e cascudo (Hypostomus plecostomus) foram examinados externamente com relação a sinais característicos de columnariose, como manchas acinzentadas na cabeça, região dorsal e pedúnculo caudal dos peixes. A amostragem compreendeu a coleta de 50 exemplares de peixes, representando as quatro diferentes espécies escolhidas para este estudo. Amostras para o isolamento foram obtidas através de raspado com swab estéril das lesões e do rim dos peixes clinicamente diagnosticados como acometidos por columnarios e imediatamente semeados em meios de culturas artificiais (líquido e sólido) próprios para o estudo de Flavobacterium segundo Carlson e Pacha (1968). No meio líquido, houve o desenvolvimento de microrganismos que observados em gota pendente apresentaram a forma de bacilos finos, longos, móveis por deslizamento. Através da coloração de Gram, apresentaram morfologia de bacilos finos, Gram negativos, agrupados em colunas. Em meio sólido, as colônias eram pequenas, cinza-amareladas, com borda em forma de raiz. No total, foram obtidos quatro isolamentos: 01 cepa de Brycon orbignyanus; 01 cepa de Piaractus mesopotamicus; 01 cepa de Colossoma macropomum; e 01 cepa de Hypostomus plecostomus. A caracterização bioquímica das amostras, como absorção do vermelho Congo, produção de flexirrubina, produção de H2S e redução do nitrato, sugere que os isolamentos poderiam ser classificados como Flavobacterium columnare.


Asunto(s)
Animales , Enfermedades de los Peces/microbiología , Infecciones por Flavobacteriaceae/veterinaria , Flavobacterium/aislamiento & purificación , Brasil , Enfermedades de los Peces/diagnóstico , Infecciones por Flavobacteriaceae/diagnóstico , Infecciones por Flavobacteriaceae/microbiología , Flavobacterium/clasificación
18.
Chinese Journal of Biotechnology ; (12): 586-591, 2008.
Artículo en Chino | WPRIM | ID: wpr-342866

RESUMEN

An isolated virulence Riemerella anatipestifer strain passaged 200 times on TSB agar were used for the virulent to avirulent conversion. The effects of passage on biological properties of outer membrane proteins (OMPs) were investigated using the virulent and avirulent strains. Transmission electron microscopy demonstrated that the avirulent strain produced lower amounts of outer membrane vesicles and the outer membrane decreased, the cytoplasmic appearance jumbled. The OMPs of the virulent strain agglutinated only in RA serotype 2 antisera, whereas the OMPs of the avirulent strain agglutinated in antisera of RA 1, 2, 10 and 11. SDS-PAGE Analysis showed the OMPs profiles of both strains were similar but the immunoblotting profiles were different. The protective immunity against Riemerella anatipestifer infection was investigated by immunizations with OMPs in ducks. ELISA results showed that the OMPs induced the production of antibodies in immunized ducks, but the OMPs of virulence strain induced higher antibody titers than the attenuated strain (P < 0.05). RA2 group showed significantly higher survival rates (100%) than RA200 group (0%) after challenged with the homologous virulent strain. The ompA gene of both stains were also amplified by PCR, nucleotide homology was 99.9%. In conclusion, OMPs of virulent RA strain are suitable candidates for vaccine development. Biological properties of OMPs undergoes significant changes during serial passage and suggest that vigilance should be used when extrapolating data obtained from the study of high-passage strains.


Asunto(s)
Animales , Proteínas de la Membrana Bacteriana Externa , Química , Genética , Alergia e Inmunología , Patos , Flavobacteriaceae , Química , Clasificación , Alergia e Inmunología , Infecciones por Flavobacteriaceae , Alergia e Inmunología , Microbiología , Serotipificación
19.
Indian J Pathol Microbiol ; 2006 Apr; 49(2): 293-5
Artículo en Inglés | IMSEAR | ID: sea-74251

RESUMEN

Chryseobacterium meningosepticum is an uncommon pathogen causing meningitis. We report a case of adult meningitis caused by chryseobacterium meningosepticum in an 88 year old woman. Immunosuppression due to old age, diabetes mellitus and history of hypertension of 20 years duration were the concomitant factors. chryseobacterium meningosepticum was isolated both from the cerebrospinal fluid and blood cultures. This organism was sensitive to quinolones, rifampicin and resistant to many antibiotics commonly used for empiric therapy for meningitis.


Asunto(s)
Anciano , Anciano de 80 o más Años , Chryseobacterium/efectos de los fármacos , Farmacorresistencia Bacteriana , Resultado Fatal , Femenino , Infecciones por Flavobacteriaceae/etiología , Humanos , Meningitis Bacterianas/etiología
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