Inflammatory response of Haemophilus influenzae biotype aegyptius causing Brazilian Purpuric Fever

The Brazilian Purpuric Fever (BPF) is a systemic disease with many clinical features of meningococcal sepsis and is usually preceded by purulent conjunctivitis. The illness is caused by Haemophilus influenza biogroup aegyptius, which was associated exclusively with conjunctivitis. In this work construction of the las gene, hypothetically responsible for this virulence, were fusioned with ermAM cassette in Neisseria meningitidis virulent strains and had its DNA transfer to non BPF H. influenzae strains. The effect of the las transfer was capable to increase the cytokines TNFα and IL10 expression in Hec-1B cells line infected with these transformed mutants (in eight log scale of folding change RNA expression). This is the first molecular study involving the las transfer to search an elucidation of the pathogenic factors by horizontal intergeneric transfer from meningococci to H. influenzae.

Studies on haemophilus species in raw milk

A total of 100 raw milk samples were collected from buffalo farms and retail stores [50 samples each] for the detection of Hemophilus species, 20% of the examined samples were contaminated with hemophilia, 11 samples [22%] from buffalo farms and 9 [18%] from retail stores. The isolated species were H. influenzae [6%], H. parainfluenzae [5%], H. segnis [4%], H. aphrophilus [3%] and H. parphrophilus [2%]. Heat treatment of milk either by pasteurization or boiling was sufficient to inactivate all the isolates of hemophilia. Addition of different antibiotics as a supplement to the growth medium resulted in the isolation of hemophilia with different rates. Public health importance of recovered hemophilia has also been discussed