Células tronco do tecido adiposo de equinos: Estudo do seu potencial para o tratamento da endometrose / Stem cells from equine adipose tissue Study of their potential for the treatment of endometrosis

A aplicação terapêutica de Células Tronco ( CT) em equinos é um campo emergente. Nestes animais, as CT são promissoras para o tratamento de lesões nos tendões e rupturas de ligamentos...

In horses, Stem Cell (SC) therapies are a promising tool to the treatment of many injuries, as tendon lesions and ligaments rupture...

Antisperm antibodies in women with unexplained infertility

Unexplained infertility occurs in many couples of childbearing age, immune mechanisms have been postulated in this disorder for the last few decades. Circulating antibodies against spermatozoa present in serum and seminal plasma have been especially implicated. This autoimmunity against spermatozoa has been investigated in males, while the isoimmunity [in the females] has got low attention. Fifty women with unexplained infertility and twenty fertile women were involved in this case-control study. ELISA test was used to detect antisperm antibody [ASA] from cervical mucus [CM] and serum specimens of both groups of women. CM was collected at mid-cycle period and dissolved mechanically [not by bromeline]. Thirty percent of infertile women have IgG-ASA in their serum and 20% have IgA-ASA in the CM, while 22% of fertile women have IgG-ASA in their serum and no fertile women have any titer of IgA-ASA in their CM specimens. Only CM-IgA-ASA of infertile women showed significant statistical correlation with cellular property of CM, which was scored according to Insler score. It is concluded that ELISA test is more sensitive and specific than microagglutination tests for detection of serum and secreted ASA. Also, secretory IgA-ASA are more indicative amid have potential role in immunological infertility as iso- immunity than IgG-ASA. Therefore, it is strongly recommended that immunological infertility should be considered as an important cause of infertility and have to gain a special interest by clinicians

[Comparison of anti Chlamydia antibodies in tubal and non-tubal infertile patients]

Chlamydia trachomatis infection is one of the most prevalent bacterial sexually transmitted infection in most countries. This organism may stay in genital tract for long time and cause subtle yet progressive damage in fallopian tubes. In this study we evaluate the correlation between chlamydia antibodies and tubal and other factors of infertility. In this case control study, 28 patients with tubal factor infertility, 28 patients with non tubal factor infertility and 30 normal patients were enroled. Presence or absence of tubal factor was assessed by direct vision via laparscopy, then titres of IgA and IgG were evaluated in all of them using ELISA method in the same labratory. Data were recoded and analyzed using SPSS software and chi-square, Fisher's exact, T-test and Mann- Whitney test. Positive titre of IgG was higher in tubal factor infertility but it was not statistically significant between three groups [p>0.294]. Positive titres of IgA were more common in non tubal factor infertility [p=0.007]. Though positive and negative titres of IgA [P=0.224] and IgG [P=0.273] were not statistically different in fertile and infertile patients. Positive and negative titres of IgA and IgG were also not statistically different in patients with or without PID [p>0.05]. No correlation was found between the positive titres of IgG and IgA against Chlamydia and tubal factor infertility

Identification of bovine sperm specific polypeptides reactive with antisperm antibodies.

The present study was taken to characterize molecular weights of sperm specific polypeptides antigenic to rabbits and calf with the aim to assess their immunoreactivity with IgG antibodies in sera from immuno-infertile cows. Seropositivity for antisperm IgG antibodies in 75 repeat breeder and 15 pregnant control cattle was tested by cellular ELISA using washed spermatozoa antigen from 4 bulls. Molecular weights of bovine sperm polypeptides antigenic to rabbit and calf were determined by 10% SDS-PAGE and Western blotting. Molecular weights of sperm peptides reactive with sera from immuno-infertile cows were also determined. Seropositivity of antisperm IgG antibodies for bull I, II, III and IV was 23.6, 14.6, 26.6 and 20%, respectively. A total of 16 polypeptides were discernible on gel. Out of these, 7 polypeptides were immunoreactive with sera from hyperimmunized rabbits as compared to 3 poly-peptides which reacted with sera from hyper-immunized calf. Only two polypeptides were reactive with sera from immuno-infertile cows. Variable number of sperm polypeptides and their immunoreactivity have been reported in different species. Antigenicity of different polypeptides in sperm needs further investigations.

Aspectos inmunológicos de la endometriosis / Immunological aspects of endometriosis

Es claro el efecto que la endometriosis en etapas avanzadas tiene sobre la fertilidad, por las alteraciones adherenciales sobre trompas y ovarios; pero es más importante comprender los mecanismos fisiopatológicos por las cuales se presenta la infertilidad en la endometriosis mínima y leve. Aunque se desconoce la causa específica de la endometriosis, los componentes del sistema inmunitario pueden estar afectados y jugar un papel central en la fisiopatogenia de la enfermedad, al alterar el microambiente peritoneal. Por un lado, el aumento de subpoblaciones de leucocitos y su actividad en líquido peritoneal reflejan efectos citotóxicos contra espermatozoides, oocito, interación de gametos y desarrollo embrionario temprano. Por otro lado, la secreción de citocinas y factores de crecimiento que favorecen o estimulan el crecimiento de focos endometrióticos en un ambiente de ®tolerancia inmunológica¼. Se revisan los aspectos inmunológicos de la endometriosis, incluyendo las vías paracrina o autocrina de comunicación entercelular

Chlamydia trachomatis y esterilidad por obstrucción tubaria / Chlamydia trachomatis and sterility by tubal obstruction

Se estudió la presencia de IgG e IGM específicas contra C. Trachomatis en dos poblaciones de mujeres, una de embarazadas y otra de estériles con obstrucción tubaria comprobada por histerosalpingografia y/o laparoscopía. Se empleó un método de inmunofluorescencia indirecta sobre células MacCoy infectadas con el serotipo L2 BU434 de C. Trachomatis. El 15,6 por ciento (5/32) de las mujeres estériles presentó IgM específica anti-C. trachomatis y el 75 por ciento (24/32) IgG. El 4,8 por ciento (4/83) de las embarazadas presentó IgM y el 20,5 por ciento (17/83) IgG. La frecuencia de detección de IgG anti-C. trachomatis fue significativamente mayor (p < 0,000) en la población de mujeres estériles que en la de embarazadas. Además, las medias geométricas del título de anticuerpos en mujeres estériles fue mayor (90) que en las fértiles (70). En 12 de 14 (85 por ciento) mujeres estériles con serología positiva se observaron imágines laparoscópicas compatibles con infección por C. trachomatis. Se detectó antígeno de clamidia en 2 de 10 biopsias de trompas por inmunofluorescencia con anticuerpos monoclonales, siendo todos los aislamientos negativos. Nuestros resultados señalan la importancia de C. trachomatis en la esterilidad por obstrucción tubaria y la necesidad de incorporar a los análisis ginecológicos del diagnóstico de este agente a fin de prevenir la infección neonatal y los riesgos de esterilidad

Sperm antibodies and unexplained infertility in females

Immunological factors as a possible cause for unexplained infertility was investigated in 50 infertile patients and a Similar number of women taken as a control The investigated [positive sperm immobilizing antibody test [SIT] was Significantly higher among the infertile group than the control. This significant Association was not found with the sperm agglutinating antibody test [SAT]

Infertilidad inmunológica / Inmunologic infertility

Fueron estudiadas 110 parejas con infertilidad primaria o secundaria mayor de 12 meses de evolución, seleccionándose 50 varones y 64 mujeres con infección genital. Se estudió la presencia de Anticuerpos Anti Espermáticos (AAE) en suero, plasma seminal, secreción prostática post-masaje y moco cervical por el teste de aglutinación en bandeja y el test e inmovilización espermática, hallándose solamente presentes los AAE en suero de 5 hombres (4 portadores de prostatitis y 1 portador de uretritis) y 2 mujeres (portadoras de cervicitis)

CA 125 levels in patients with schistosomiasis infertility

Twenty-five women with schistosomiasis infertility and 20 fertile women were included in this study for estimation of CA 125. There was statistically significant increase of CA 125 in Bilharzial infertility group [mean 46.93 U/ml] as compared to the in control group [mean-18.23 U/ml]. Follow-up laparoscopically and serologically revealed increased mean value after 6 months and after one year [52.12 U/ml. and 67.46 U/ml respectively]. This increase run in parallel with graduation of endometriosis from occult to manifest endometriosis, so cases with bilharzial infertility may be a subject of occult endometriosis which can be detected by CA 125

Fator imunológico e infertilidade: I. Testes de interaçäo e muco-sêmen / Immunologic factor and infertility: I. Sperm-cervical mucus interaction tests

Procura-se analisar a propedêutica básica no rastreamento do fator imunológico, como causa de infertilidade. Säo avaliados o teste pós-coito, segundo as características do muco cervical e dos espermatozóides, e os testes "in vitro" de penetraçäo em lâmina e em tubo capilar (Kremer)

Esterilidad producida por endometiosis / Esterility due to endometiosis

Se ha tratado de interpretar las posibles causas de esterilidad por endometriosis. Se recuerda la definición de endometriosis externa y de estrogenismo sostenido. Se trata cono único tema la probable etiología de la esterilidad producida por endometriosis, dejando de lado aquellas que pueden llegar a ocasionar la endometiosis externa. Motivos de esterilidad por endometriosis externa: a) Factores ambientales, familiares y genéticos, de gran importancia etiológica, pero desafortunadamente hasta el presente estudiados en forma incompleta. b) Alteraciones hipófiso-tiroides-ovario, en particular las producidas por hipotiroidismo a nivel ovárico y endometrial. c) problemas inmunológicos demostrados en: Mucosa endometrial, placas y quistes endometriósicos, así como en la endometriosis primaria del ovario. d) Factores mecánicos y adherenciales que favorecen bloqueos: ováricos y pelvis congelada