RESUMEN
La lectina de unión a la manosa (MBL) es una colectina que se sintetiza en el hígado y es secretada al torrente sanguíneo, la cual es capaz de unirse con estructuras repetidas de azúcares presentes en una amplia variedad de bacterias y otros microorganismos promoviendo su eliminación mediante la activación del complemento a través de serín proteasas asociadas. A las deficiencias de MBL se les considera como un importante factor de riesgo de infecciones en niños y en individuos inmunosuprimidos. Se discute la evidencia de que la MBL contribuye de forma importante a la inmunidad innata con el incremento de la susceptibilidad a determinadas enfermedades o la incidencia en el curso de estas. Estudios preliminares del empleo de terapias sustitutivas con MBL han arrojado resultados prometedores, los que deben ofrecer evidencias acerca del significado fisiológico de esta proteína
Asunto(s)
Humanos , Lectina de Unión a Manosa/fisiología , Lectina de Unión a Manosa/genética , Lectina de Unión a Manosa/química , Lectina de Unión a Manosa/uso terapéuticoRESUMEN
Using RNA extracted from Zantedeschia aethiopica young leaves and primers designed according to the conservative regions of Araceae lectins, the full-length cDNA of Z. aethiopica agglutinin (ZAA) was cloned by rapid amplification of cDNA ends (RACE). The full-length cDNA of zaa was 871 bp and contained a 417 bp open reading frame (ORF) encoding a lectin precursor of 138 amino acids. Through comparative analysis of zaa gene and its deduced amino acid sequence with those of other Araceae species, it was found that zaa encoded a precursor lectin with signal peptide. Secondary and three-dimensional structure analyses showed that ZAA had many common characters of mannose-binding lectin superfamily and ZAA was a mannose-binding lectin with three mannose-binding sites. Southern blot analysis of the genomic DNA revealed that zaa belonged to a multi-copy gene family
Asunto(s)
Lectina de Unión a Manosa/fisiología , Lectina de Unión a Manosa/genética , Lectina de Unión a Manosa/química , Lectina de Unión a Manosa , Proteínas de Plantas/fisiología , Proteínas de Plantas/genética , Proteínas de Plantas/química , Genes de Plantas/fisiología , Genes de Plantas/genética , Plantas Modificadas Genéticamente/fisiología , Plantas Modificadas Genéticamente/genética , Plantas Modificadas Genéticamente/química , Regulación de la Expresión Génica de las Plantas/fisiología , Regulación de la Expresión Génica de las Plantas/genéticaRESUMEN
Mannose-binding lectin (MBL) plays an important role in immune defense. This study was undertaken to investigate the association between hepatitis B virus infection and polymorphisms of MBL gene. We assessed the single nucleotide polymorphism at codon 54 in exon 1 of MBL in patients with hepatitis B virus infection and HBsAg negative controls in Korean population. A total of 498 enrolled subjects was classified into four groups. Group 1; Clearance, Group 2; Inactive healthy carrier, Group 3; Chronic hepatitis, Group 4; Liver cirrhosis. MBL gene polymorphisms at codon 54 led to three genotypes (G/G, G/A, A/A). When we divided subjects into clearance group (group 1) and persistence group (group 2-4), G/G genotype and A-allele carrier were observed in 55.6% and 44.4% in clearance group, 64.8% and 35.2% in persistence group (p=0.081), respectively. When hepatitis B virus persistent cases were divided into inactive healthy carrier (group 2) and disease progression group (group 3 and 4), MBL gene polymorphisms at codon 54 were not related to disease progression (p=0.166). MBL gene polymorphism at codon 54 was not associated with the clearance of hepatitis B virus infection nor progression of disease in chronic hepatitis B virus infection.