RESUMEN
Changes in the expression of genes were observed during development in populations of Anopheles (Anopheles) intermedius and Anopheles (Anopheles) mattogrossensis. Esterase showed seven zones of activity: EST1 was present in all developmental stages of both species; EST2 was observed only in larvae of A. intermedius and larvae and pupae of A. mattogrossensis, with greater activity in pupae; EST3 and EST5 were present in all development stages, with greater intensity in larvae; EST4 and EST6 showed weak activity in larvae of A. mattogrossensis and was not found in A. intermedius. Leucine aminopeptidase showed four zones of activity, of which LAP1 and LAP2 were found in all stages of A. intermedius, with highest activity in larvae, and in only of A. mattogrossensis. LAP3 was detected in all stages of A. mattogrossensis and in larvae only of A. intermedius. LAP4 was detected only in larvae and pupae of A. mattogrossensis, with greater intensity in pupae. Alpha-Glycerophosphate dehydrogenase showed a single zone of activity, detected in older fourth-instar larvae and becoming more intense from the pupal stage onwards.
Asunto(s)
Animales , Anopheles/genética , Variación Genética , Anopheles/enzimología , Brasil , Electroforesis , Esterasas/genética , Esterasas/metabolismo , Regulación Enzimológica de la Expresión Génica , Glicerolfosfato Deshidrogenasa/genética , Glicerolfosfato Deshidrogenasa/metabolismo , Leucil Aminopeptidasa/genética , Leucil Aminopeptidasa/metabolismoRESUMEN
The esterases, leucine aminopeptidase and alpha-glycerophosphate dehydrogenase revealed modifications in gene expressions during the development of Anopheles darlingi. The esterases showed five activity bands, 1 and 2 being more deeply stained during the larval stages than in pupae or adults, esterases 3 and 4 more deeply stained in pupae and adults whereas esterase 5 was present throughout development. Leucine aminopeptidase showed five activity bands: LAP2 and LAP5 were characteristic of larvae, LAP3 was specific for pupae and adults, LAP4 was detected only in pupae, and LAP1 and LAP6 were detected in all stages. Alpha-Glycerophosphate dehydrogenase presented one activity band on starch gel whose intensity increased with development. Two activity bands were detected on polyacrylamide gel (alpha-GPDH1 and alpha-GPDH2) in 4th-instar larvae (old pigmented larvae) and this activity increased with development.
Asunto(s)
Animales , Anopheles/genética , Esterasas/genética , Expresión Génica , Variación Genética , Glicerolfosfato Deshidrogenasa/genética , Leucil Aminopeptidasa/genética , Anopheles/enzimología , Anopheles/crecimiento & desarrollo , Electroforesis en Gel de Poliacrilamida , Electroforesis en Gel de Almidón , Esterasas/metabolismo , Glicerolfosfato Deshidrogenasa/metabolismo , Isoenzimas/genética , Isoenzimas/metabolismo , Leucil Aminopeptidasa/metabolismoRESUMEN
Three groups of Aedes aegypti maintained 8 generations in the laboratory at 20 degrees C, 27 +/- 1 degree C (normal control), and at 35 degrees C, respectively were analyzed for allozyme variability. Of the 8 larval loci examined Est alpha-1 and Lap-2 in generation 8 were still variable, while the other allozymes became uniform. The 35 degrees C population had mean level of heterozygosity of about 6% of individual per locus, less than those observed in the 20 degrees C and the control populations which were 14% and 13%, respectively. It is suggested that in the experimental populations, the rate of loss of alleles can be increased by high temperature which must not be higher than the optimum temperature of enzymes.