Aspectos ultraestruturais da permeabilidade do mesentério de rato a exposiçâo ao ferro coloidal / Ultrastructural aspects of permeability in mouse mesentery expossed to coloidal iron

In previous we could observe that many researches were interested in the phenomena of absorption that occur in the mesothelial that involves the viscerae. We already made some researches empliying some chemical substances like the lanthaum nitrate and the osmium amine. With the utilization of the lanthanum nitrate we came to the conclusion that the penetration of this heavy salt is impeded by the presence of the basement membrane that separates the mesothelial from the connective tissue. This membrane, as observed at that time, is continous that separates the mesothelial from the connective tissue. This membrane, as observed at that time, is continous with the basal lamina located between mesothelial cells. Our observations led us to the conclusion that the salt traveled through the cytoplasm and not in between neighbouring mesothelial cells. The observations made in the material treated with osmjium-amine, were comapred to those made with the employment of the lanthanum nitrate. In general, no reaction was observed in any of the techniques employed, with the exception of some special cases. Int he later the osmium amine reacts in the outer surface of the mesentery but also penetates with no visible reaction, all the way to the connective tissue to react with the elastic layer. In the present paper, where tha colloidal iron was employed, int he mesothelial cells and in the connective tissue. We are comming to a final conclusion that the permeability of different layears of tissues is of a great variety and of a great capacity of selectiveness

Electron Microscopic Observations on the Morphological Changes of Rat Mesentric Mast Cells Induced by Morphine HCl

The effects of morphine HCI on the rat mesenteric mast cells were studied with the electron microscopy. The materials were prepared for electron microscopy by osmium tetroxide fixation and embedding in Epon. The rat mesenteric mast cells showed no distinct morphological changes due to morphine HCl, but the mast cell granlues were changed in various ways. For instance, they formed dusters, showed granular lysis, and an appearance of electron transparency. Frequently, some granules appeared in the extracellular space and the boundary of the granules was not evident. From the results mentioned above, it was suggested that rat mesenteric mast cell granules were affected by morphine HCl in the shape, the granular matrix, and the granular boundaries.