RESUMEN
A method was evaluated for determination of twenty-one doping (including nandrolone, boldenone and methandienone) in human urine by gas chromatography-high resolution mass spectrometry. Samples were prepared by liquid-liquid extraction, concentrated, TMS derivatization and limit of detection at ng x mL(-1) by MID/GC/HRMS. According to the code of the World Anti-Doping Agency (WADA), precision and recoveries of the procedure were evaluated by replicate analysis (n = 6), the recoveries in the range of 66%-103%, with the RSD below 10.0%. The precision within the day of the method with three different concentrations was also determined RSD were less than 9.5%, 10.0% and 9.7%.
Asunto(s)
Humanos , Anabolizantes , Orina , Doping en los Deportes , Cromatografía de Gases y Espectrometría de Masas , Metandrostenolona , Orina , Nandrolona , Orina , Detección de Abuso de Sustancias , Métodos , Testosterona , OrinaRESUMEN
The influence of anabolic steroid on the process of production of rickets in vitamin-D deficient animals as well as its influence on healing of rickets with vitamin-D therapy was studied. Long-Evans strains of female rats of two age groups (6 weeks and 4 weeks) were given rachitogenic diet and were divided into five sub-groups-(I) control receiving vitamin-D from the 1st day, (II) rachitic group, (III) receiving vitamin-D from 36th day, (IV) receiving anabolic hormone from the beginning of the experiment and (V) receiving both vitamin-D and anabolic hormone from 36th day of treatment. It was observed grossly as well as histologically and radiographically that: (1) the anabolic steroid minimizes the rachitic changes in the bones of vitamin-D deficient rats, (2) in rachitic animals, a combination therapy of anabolic hormone and vitamin-D results in comparatively greater calcification of osteoid matrix and better healing and remodelling of bone than with vitamin-D alone.
Asunto(s)
Animales , Quimioterapia Combinada , Epífisis/patología , Femenino , Metandrostenolona/uso terapéutico , Ratas , Raquitismo/tratamiento farmacológico , Tibia/patología , Vitamina D/uso terapéuticoAsunto(s)
Adolescente , Adulto , Antropometría , Apetito , Brazo/anatomía & histología , Presión Sanguínea , Peso Corporal/efectos de los fármacos , Estudios de Seguimiento , Humanos , Masculino , Metandrostenolona/farmacología , Esfuerzo Físico , Pulso Arterial , Grosor de los Pliegues Cutáneos , Muslo/anatomía & histologíaAsunto(s)
Humanos , Masculino , Femenino , Metandrostenolona , Resultado del Tratamiento , Degeneración MacularRESUMEN
Se presenta un paciente de seis años de edad, afectado de anemia hipoplástica congénita. Se efectúa un análisis de la etiopatogenia, diagnóstico, pronóstico y de las posibilidades terapéuticas.