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1.
Acta cir. bras ; 36(3): e360304, 2021. tab, graf
Artículo en Inglés | LILACS, VETINDEX | ID: biblio-1284908

RESUMEN

ABSTRACT Purpose To evaluate the effect of fractional carbon dioxide (CO2) laser treatment using lower power associated with pulse stacking within collagen fibers, using second harmonic generation microscopy and computerized image analysis. Methods Twenty male Wistar rats aging eight weeks were used. Each treatment area received a single-pass CO2 fractional laser with different parameters. The 20 animals were divided into two groups and euthanized after 30 and 60 days. Second harmonic generation images were obtained and program ImageJ was utilized to evaluate the collagen organization within all areas. Collagen anisotropy, entropy and optical density were quantified. Results Increased anisotropy over time was observed in all four areas, but only reached statistical significance (p = 0.0305) when the mildest parameters were used (area four). Entropy decreased over time in all areas, but without significance(p = 0.1779) in area four. Density showed an overtime increase only in area four, but no statistical significance was reached (p = 0.6534). Conclusions When combined, the results obtained in this study regarding anisotropy, entropy and density tend to demonstrate that it is possible to achieve collagen remodeling with the use of lower power levels associated with stacked pulses.


Asunto(s)
Animales , Masculino , Ratas , Envejecimiento de la Piel , Láseres de Gas , Microscopía de Generación del Segundo Armónico , Piel , Dióxido de Carbono , Ratas Wistar
2.
Acta cir. bras ; 36(3): e360304, 2021. tab, graf
Artículo en Inglés | LILACS | ID: biblio-1248536

RESUMEN

ABSTRACT Purpose To evaluate the effect of fractional carbon dioxide (CO2) laser treatment using lower power associated with pulse stacking within collagen fibers, using second harmonic generation microscopy and computerized image analysis. Methods Twenty male Wistar rats aging eight weeks were used. Each treatment area received a single-pass CO2 fractional laser with different parameters. The 20 animals were divided into two groups and euthanized after 30 and 60 days. Second harmonic generation images were obtained and program ImageJ was utilized to evaluate the collagen organization within all areas. Collagen anisotropy, entropy and optical density were quantified. Results Increased anisotropy over time was observed in all four areas, but only reached statistical significance (p = 0.0305) when the mildest parameters were used (area four). Entropy decreased over time in all areas, but without significance(p = 0.1779) in area four. Density showed an overtime increase only in area four, but no statistical significance was reached (p = 0.6534). Conclusions When combined, the results obtained in this study regarding anisotropy, entropy and density tend to demonstrate that it is possible to achieve collagen remodeling with the use of lower power levels associated with stacked pulses.


Asunto(s)
Animales , Masculino , Ratas , Envejecimiento de la Piel , Láseres de Gas , Microscopía de Generación del Segundo Armónico , Piel , Dióxido de Carbono , Ratas Wistar
3.
São Paulo; s.n; 2020. 50 p. ilust.
Tesis en Portugués | LILACS, Inca | ID: biblio-1178971

RESUMEN

Introdução: A matriz extracelular (MEC) desempenha um papel importante na função celular, sendo que seu componente mais importante são as fibras de colágeno (COL). Nos últimos anos, a Microscopia de Geração Segundo Harmônico (em inglês, second harmonic generation microscopy; SHG) tem sido utilizada para a análise de COL em vários tipos de cânceres ginecológicos, como o da mama e ovário. Desta forma, tem-se enfatizado o valor da quantificação do colágeno por este método como meio de aprofundar o conhecimento da fisiopatologia e do prognóstico na avaliação destas neoplasias. Objetivo: No presente estudo caracterizamos as fibras de COL utilizando-se a microscopia de SHG objetivando avaliar diferenças entre carcinoma de células escamosas e lesões pré-neoplásicas da vulva, bem como verificar se há relação entre características dessas fibras com parâmetros clinicopatológicos associados a prognóstico. Método: Trata-se de um estudo de coorte horizontal e retrospectivo. Foram incluídos 52 pacientes, entre os anos de 2000 a 2010, sendo 40 com Carcinoma de Células Escamosas (CCE) Vulvar; 12 pacientes com Neoplasia Intraepitelial Vulvar (NIV); e, para o controle de comparação interna, foram considerados tecidos distantes ao tumor sem presença de lesão tumoral e/ou NIV. Para análises laboratoriais, foi selecionado um espécime representativo do processo patológico para revisão diagnóstica; e, posteriormente, submetido ao estudo do COL usando a microscopia de SHG. Os tecidos adjacentes normais e intratumorais foram selecionados em secções, para a avaliação do COL, e corados em Hematoxilina/Eosina (H&E) e analisados por microscopia confocal. Parâmetros relacionados ao colágeno, como quantidade, organização e uniformidade, em áreas de lesões neoplásicas e pré-neoplásicas da vulva, foram comparados com áreas distantes de aspecto normal das mesmas pacientes. Resultado/conclusão: Foi observado um evidente decréscimo nos valores dos parâmetros do COL no estroma associado às lesões pré-neoplásicas e neoplásicas da vulva. Além disto, aumentos na quantidade e uniformidade das fibras do colágeno tumorais se associaram à presença de metástases linfonodais, um reconhecido parâmetro de pior prognóstico no carcinoma de vulva


Background: The extracellular matrix (ECM) plays an important role in cellular function, being collagen fibers its most important component. Over the last few years, second harmonic generation (SHG) microscopy has been used for the analysis of collagen fibers in several types of cancers, including breast and ovarian cancer. The value of collagen parameters obtained using this technique has been advocated to gain insights on the physiopathology and on the prognostic evaluation of cancer. Objective: Herein, we have characterized collagen fibers using the SHG microscopy, to evaluate differences between vulvar cancer and preneoplastic lesions, and to find possible associations between characteristics of collagen fibers with clinicopathological parameters related to prognosis. Methods: This is a retrospective and horizontal cohort study. Were included 52 patients, between the years 2000 to 2010, 40 of whom with Vulva Squamous Cell Carcinoma (VSCC); 12 patients with Vulvar Intraepithelial Neoplasia (VIN); and, for the control of internal comparison, tissues distant to the tumor without tumor lesion and / or VIN were considered. For laboratory analysis, a specimen representative of the pathological process was selected for diagnostic review and subsequently subjected to the study of collagen using SHG microscopy. The normal and intratumoral adjacent tissues were selected in sections, for collagen evaluation, and stained in Hematoxylin/Eosin (H&E) and analyzed by confocal microscopy. Collagen parameters, quantity, organization, and uniformity, of stroma adjacent to neoplastic or preneoplastic lesions were compared with values obtained in normal appearing tissue distant from those lesions from the same patients. Results/Conclusion: There was an evident decrease in the values of collagen fiber parameters in the stroma associated to tumors. Increased quantity and uniformity of tumor associated collagen fibers were associated with the presence of lymph node metastases, which suggest a prognostic value of such parameters in the evaluation of vulvar cancer


Asunto(s)
Humanos , Femenino , Adulto , Persona de Mediana Edad , Anciano , Lesiones Precancerosas , Neoplasias de la Vulva , Colágeno , Matriz Extracelular , Microscopía de Generación del Segundo Armónico , Metástasis de la Neoplasia
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