Microbiología de las infecciones causadas por mordeduras de perros y gatos en personas: una revisión / Microbiology of infections caused by dog and cat bites: a review

INTRODUCCIÓN: Las mordeduras causadas por animales de compañía constituyen el 5% de las heridas traumáticas registradas en los servicios de urgencias. OBJETIVO: Conocer los principales agentes infecciosos presentes en las mordeduras provocadas por perros y gatos, tanto de forma individual como conjunta; así como los factores que favorecen la infección. METODOLOGÍA: Se realizó una búsqueda bibliográfica en Pub-Med con la siguiente estrategia de búsqueda: (("Bites, Human"[Mesh]) OR "Bites and Stings"[Mesh]) AND "Infection"[Mesh]. Se incluyeron 24 trabajos en la síntesis cualitativa escritos en lengua inglesa o española, casos clínicos o descriptivos y publicados entre los años 2000 y 2019. RESULTADOS: Las especies más frecuentemente aisladas fueron Capnocytophaga canimorsus en perros y Pasteurella multocida en gatos. La existencia de comorbilidades en el individuo, la mordedura en la mano, o la presencia de signos de alteración del estado general del individuo mordido fueron considerados como factores de riesgo para el desarrollo de la infección tras la mordedura. CONCLUSIONES: Todos los afectados por mordeduras animales deben recibir asistencia médica y considerar la administración de una pauta de profilaxis antimicrobiana con el fin de reducir el riesgo de shock séptico. Por otro lado, es importante advertir al laboratorio de microbiología de la naturaleza de las muestras clínicas obtenidas para alcanzar el mejor diagnóstico etiológico.

BACKGROUND: Bites caused by pets constitute 5% of the traumatic injuries registered in the emergency services. AIM: To know the main infectious agents present in dog and cat bites, both individually and jointly, in humans, as well as the predisposing factors that favor infection and its spread. METHODS: A bibliographic search was carried out in PubMed with the following search strategy: (("Bites, Human" [Mesh]) OR "Bites and Stings" [Mesh]) AND "Infection" [Mesh]. Twenty-four papers were included in the qualitative synthesis written in English or Spanish, clinical or descriptive cases and published between 2000 and 2019. Results: Most frequently isolated species were Capnocytophaga canimorsus in dogs and Pasteurella multocida in cats. The existence of comorbidities in the individual, the bite on the hand, or the presence of signs of alteration of the general state of the bitten individual were considered as risk factors for the development of infection after the bite. CONCLUSIONS: All patients with animal bites should receive medical assistance, and the administration of an antibiotic prophylaxis regimen should be considered to reduce the risk of septic shock. Besides, it is important to advise the microbiology laboratory of the nature of the clinical samples obtained in order to reach the best etiological diagnosis.

Oral microbiota and their antibiotic susceptibility in free-living monkeys in Goiás State, Brazil: Repercussions for injuries in humans

Abstract INTRODUCTION: Goiás State, which is in the midwest region of Brazil, has several urban forests. This fact, along with the expansion of urban areas within the limits of Forest Conservation Units, increases the contact between humans and wildlife, such as capuchin monkeys. The impulsive behavior of these animals and the scarcity of food cause them to vigorously search for food, leading to direct encounters with Park visitors, which can result in scratches and bites and making them potential disseminators of pathogenic microorganisms. METHODS: Ten specimens of bearded capuchin monkey (Sapajus libidinosus) were captured at the Onofre Quinan Environmental Park in Anápolis, Goiás, Brazil. Samples were collected from the monkeys, and the bacteria and fungi present in the samples were isolated and identified. Then, the identified microorganisms were subjected to antibiotic susceptibility testing. RESULTS: A total of 111 bacteria and 12 fungi were isolated, including two strict anaerobic bacteria of the genus Peptostreptococcus, 109 facultative anaerobic bacteria, and 12 yeasts. Among the facultative bacteria, enterobacteria and Staphylococcus were common. Resistance to tetracycline and ampicillin antibiotics was detected in the enterobacteria, and resistance to tetracycline, erythromycin, and clindamycin was detected in the Staphylococci. The other strains were sensitive to all tested antimicrobials. Cefoxitin showed 100% efficacy in all isolated bacteria. CONCLUSIONS: For bites from capuchin monkeys, we recommend performing complete hygiene and antibiotic therapy, according to medical recommendations. Given the 100% effectiveness of cefoxitin, it should be considered for this type of injury, especially in the study region.

Microbiología de las infecciones posteriores a mordeduras / Microbiology of bite wound infections

Son ampliamente conocidos por los pediatras los procedimientos a seguir ante las heridas inflingidas a un niño por perros, gatos o por otros seres humanos. En esta actualización se detallan los microorganismos causales de infecciones posteriores a mordeduras, enfatizando aquellos vinculados a agresiones de otros animales. En casos de mordeduras por animales de granja, salvajes (en especial reptiles) y acuáticos es necesario considerar la inclusión, en el esquema profiláctico empírico inicial, antibióticos activos frente a enterobacterias, Aeromonas, Vibrio y Pseudomonas. En el caso de mordeduras de monos se debe tener en cuenta la posibilidad de infecciones virales potencialmente fatales, en las provocadas por roedores, la esporotricosis y en las producidas por animales acuáticos, las micobacterias atípicas como Mycobacterium marinum o Mycobacterium haemophilum, dado que desarrollan en medios para micobacterias pero a 30ºC (AU)

The adequate procedures to follow in children with bite wounds inflicted by dogs, cats, or by other human beings are well known by pediatricians. This update describes microorganisms that may cause infections in bite wounds, with a focus on those caused by other animals. In bite injuries caused by farm, wild (especially reptiles), and aquatic animals, antibiotics effective against Enterobacteriaceae, Aeromonas, Vibrio, and Pseudomonas should be included in the initial empirical prophylactic treatment scheme. In case of bite injuries caused by monkeys, the possibility of potentially fatal viral infections should be taken into account, in the case of bites by rodents the possibility of sporotricosis should be considered, and in bite wounds inflicted by aquatic animals atypical mycobacteria, such as Mycobacterium marinum or Mycobacterium haemophilum, should be taken into account as they develop in an environment for mycobacteria, but at 30ºC (AU)

Rabia en Potos flavus identificados en el departamento de madre de dios, Perú / Rabies in Potos flavus identified in Madre de Dios, Peru

El Potos flavus es un mamífero nocturno que habita en bosques neotropicales desde Centroamérica hasta Sudamérica. Se realizó un estudio de cuatro casos de rabia en Potos flavus ocurridos desde abril de 2012 en el departamento de Madre de Dios en Perú, captados como parte de la vigilancia epidemiológica. Los análisis realizados en el laboratorio de referencia regional de Madre de Dios determinaron presencia de antígeno del virus de la rabia en tres de las muestras de tejido encefálico, dichos resultados fueron corroborados en el Laboratorio de Zoonosis Virales del Instituto Nacional de Salud del Perú mediante inmunofluorescencia directa, la tipificación no identificó ninguna de las variantes conocidas en murciélagos o en perros. La ocurrencia de cuatro casos de rabia en Potos flavus suma evidencias de la emergencia de un nuevo reservorio del virus de la rabia y que ha sido reportada previamente en el mismo departamento el año 2007.

The Potos flavus is a nocturnal mammal that lives in neotropical forests from Central America to South America. A study of four cases of rabies in Potos flavus was conducted; these occurred in April 2012 in the Madre de Dios region in Peru and were collected as part of epidemiological surveillance. The analysis performed in the regional reference laboratory of Madre de Dios determined the presence of the rabies virus antigen in three of the brain tissue samples. Results were verified in the Laboratory of Viral Zoonoses of the Peruvian National Institute of Health by direct immunofluorescence. The typification did not identify any of the known variants in bats or dogs. The occurrence of four cases of rabies in Potos flavus adds evidence of the emergence of a new reservoir of the rabies virus previously reported in the same region in 2007.

Aeromonas hydrophila wound infection following a tiger bite in Nepal.

Aeromonas hydrophila is a rare human pathogen. Reports of zoonotic infection developing after large feline bites are even rarer. We are documenting the first case of human wound infection with A. hydrophila following a tiger bite. The patient responded well following wound debridement, secondary suturing and combination antibiotic therapy.

Recomendaciones para el manejo de mordeduras ocasionadas por animales / Guidelines for management of animal bites

Las mordeduras producidas por animales domésticos, silvestres o mascotas exóticas, son un problema que no está bien dimensionado en nuestro medio; sin embargo, representan un motivo de consulta frecuente. Se revisa la epidemiología, enfrentamiento inicial y recomendaciones para el manejo de mordeduras ocasionadas por perros y gatos; por la gran importancia que han ido adquiriendo en el último tiempo se revisan las mordeduras producidas por hámsters, ratones, conejos, hurones, perros de la pradera, simios, murciélagos, aves, iguanas y animales de granja.

Characterization of Orientia tsutsugamushi strains isolated in Shandong Province, China by immunofluorescence and restriction fragment length polymorphism (RFLP) analyses.

In order to identify the characteristics of the Sta56 gene of the 23 isolates of Orientia (O.) tsutsugamushi isolated in Shandong Province, indirect immunofluorescence assay (IFA) was used to identify the gene type of 23 strains O. tsutsugamushi isolated from scrub typhus patients, chigger mites, and rodents. Restriction fragment length polymorphism (RFLP) analysis was also used to analyze the restriction profiles of the Sta56 gene PCR amplification products of the 23 isolated strains of the O. tsutsugamushi; the results were compared with those acquired by nested PCR. By IFA, 21 of the 23 isolates belonged to the Gilliam type, and 2 to the Karp type. Using RFLP analysis, 21 strains had similar restriction profiles to the Japan Kawasaki strain, but they had no restriction site Hha I, and thus had some difference in gene sequence compared with the Japan Kawasaki strain. The other 2 strains had similar restriction profiles to Karp. These results were identical to that acquired by nested-PCR. In Shandong Province, the gene types of epidemic O. tsutsugamushi strains were similar to the Japan Kawasaki type, but had some differences in gene sequence. In addition, Karp also existed.

Scrub typhus during pregnancy: a case report and review of the literature.

Scrub typhus is a rickettsial disease that is uncommon during pregnancy. We report a case of a 33-year-old woman, G1P0, 29 weeks pregnancy who presented to hospital with high fever, chill and headache for two weeks. Her diagnosis of scrub typhus was confirmed by serum immunofluorescent assay. She was successfully treated with chloramphenicol, but preterm delivery occurred. Her infant died from respiratory distress syndrome. No vertical transmission was demonstrated in this case. Scrub typhus should be listed in the differential diagnosis of acute febrile illness in pregnant women, who either live in, or return from, endemic areas. Chloramphenicol can be used safely during pregnancy if it is not circulating at the time of delivery.

Oral bacterial flora of dogs with and without rabies: a preliminary study in Thailand.

The authors studied the bacterial flora of the dog oral cavity and of bite wounds, Aerobic bacteria were isolated from mouth swabs of 16 normal and 5 rabid dogs as well as from infected dog-bite wounds from 18 patients. A total of 20 different microbial species were recovered from mouth swab cultures. The most frequently isolated organisms were Klebsiella pneumoniae ssp pneumoniae, Escherichia coli, Staphylococcus aureus, Citrobacter freundii, Enterobacter cloacae, Acinetobacter calcoaceticus, and Pasteurella species. There were no differences in the aerobic bacterial flora between rabid and nonrabid dogs. From the cultures of the bite wound swabs, the authors found that almost all of the organisms identified were part of the normal oral flora of the dog. One or more aerobic bacteria were isolated from the infected dog-bite wounds. Two patients had four, 3 had three, 4 had two, and 6 had one of the nine organisms in their wounds. The predominant species of bacteria involved in infection of bite wounds were, as follows: Staphylococcus aureus, Pasteurella multocida, E. coli, Moraxella species, Pasteurella canis, and Enterobacter cloacae. However, three wound cultures had no aerobic bacterial growth. The results of this study show that the infected bite wounds may contain a mixed bacterial flora that colonize human skin and the oral cavity of dogs.

Lesiones faciales por mordeduras de humanos y de animales / Facial injuries by human and animal bites

Se presenta una revisión de la literatura así como de la experiencia en el Hospital Central Dr. Ignacio Morones Prieto en el tratamiento de las mordeduras por humanos y por animales. El tratamiento temprano a base de la revisión quirúrgica y cierre primario, así como la antiobioticoterapia adecuada, dan el éxito buscado

Quién es quién en Bacteriología: Capnocytophaga spp / Who is who in Bacteriology: Capnocytophaga spp

Dentro del género Capnocytophaga existen dos grupos de bacilos Gram-negativos de dificil crecimiento previamente conocidos como grupos DF-1 y DF-2 del CDC. En ellos se incluye a las especies C. ochracea, C. gingivalis, C. sputigena (ex DF-1), C. canimorsus y C. cynodegmi (ex DF-2 y DF-2 like). Probablemente, en el futuro, se agregue a este género otro grupo de bacterias actualmente conocidas como grupo DF-3 del CDC. Las tres primeras especies se asocian a sepsis en pacientes neutropénicos que reciben quimioterapia, aunque también son capaces de producir infecciones en huéspedes normales. C. canimorsus ha sido reconocida como agente causal de infecciones severas secundarias a contacto previo con animales en pacientes alcohólicos, esplenectomizados o tratados con corticoides. C. cynodegmi ha sido aislada de mordeduras de animales en pacientes normales. Parecería que las bacterias del grupo DF-3 podrían tener que ver con cuadros de diarrea prolongada. Es destacable además el hallazgo de C. cynodegmi en un caso de endoftalmitis en un paciente con transplante de córnea y el informe de algunos casos de infecciones perinatales producidas por bacterias del antiguo grupo DF-1. La sensibilidad de estos microorganismos es bastante amplia. La mayor experiencia clínica es la registrada con penicilina, que parece ser el antibiótico de elección. Sin embargo, es necesario destacar que se han aislado cepas de Capnocytophaga productoras de beta-lactamasas. El aislamiento de estas bacterias a partir de muestras clínicas constituye un desafío para los microbiólogos. Su desarrollo puede lograrse utilizando placas de agar tripteína de soya adicionadas de sangre al 5 por ciento e incubándolas en aero o anaerobiosis en presencia de CO2 al 5 por ciento por espacio de al menos 5 días. Su diferenciación a nivel de especie puede resultar engorrosa por la superposición de resultados en varias pruebas. De todos modos, las pruebas de oxidasa, catalasa, movilidad y arginina separan bien a los antiguos grupos DF-1 y DF-2 entre sí y de otras bacterias similares

Algunas consideraciones sobre las infecciones asociadas a mordedura humana y animal / Some considerations about infections associated with human and animal bites

Las mordeduras humanas y de animal constituyen un imprtante problema de salud y motivan un gran número de consultas, en especial en los Servicios de Urgencia, en donde se les proporciona la primera atención. Aunque lamayoría de los pacientes presenta heridas menores, por lo menos un 10 porciento necesita intervención médica posterior, ya que se complican de infección, como celulitis, artritis, osteomielitis y en ocaciones septicemia, que puede ser fatal