Henneguya sacacaensis n. sp. (Myxozoa: Myxosporea) parasitizing gills of the acará bicudo Satanoperca jurupari (Osteichthyes: Cichlidae) in eastern Amazon / Henneguya sacacaensis no. sp (Myxozoa: Myxosporea) parasitando brânquias do acará bicudo Satanoperca jurupari (Osteichthyes: Cichlidae) na Amazônia oriental

Abstract This study describes Henneguya sacacaensis n. sp. in specimens of the Osteichthyes Satanoperca jurupari (Heckel, 1840), collected in the Rio Curiaú Environmental Protection Area in the city of Macapá, state of Amapá Brazil. Using optical microscopy and molecular analysis, these cyst-shaped parasites were analyzed. The gills of 57.14% of the analyzed S. jurupari contained hundreds of spores. The cysts found on the gill lamellae were oval-shaped and whitish. The Henneguya spores had an average length of 46.5 (41.3-56.92) µm. The fusiform body of the Henneguya measured 16.5 (13.16-20.01) µm long and 5.1 (3.91-6.12) µm in width, the two polar capsules had a taper of 3.83 (3.4-4.32) µm and a width of 1.68 (1.4-1.99) µm, and the tail measured 30 (22.47-41.67) µm in length, containing a polar filament coiled seven to nine times. Morphogical and phylogenetic analysis allowed the preposition of a new species, Henneguya sacacaensis n. sp, that belongs to the family Myxobolidae and the genus Henneguya.

Resumo Henneguya sacacaensis n. sp. é descrito em espécimes do Osteichthyes Satanoperca jurupari (Heckel, 1840), coletados na área de Proteção Ambiental do rio Curiaú na cidade de Macapá no estado do Amapá, Brasil. Com auxílio de microscopia óptica e análises moleculares, esses parasitos foram analisados e observados nas brânquias em forma de cistos, contendo centenas de esporos e apresentaram a prevalência de 57,14%. Os cistos encontrados nas lamelas branquiais tinham formatos ovais e esbranquiçados. Seus esporos apresentaram um comprimento médio de 46,5 (41,3-56,92) µm, corpo fusiforme medindo 16,5 (13,16-20,01) µm de comprimento e 5,1 (3,91-6,12) µm de largura, suas duas cápsulas polares apresentam uma conicidade de 3,83 (3,4-4,32) µm e sua largura 1,68 µm (1,4-1,99), a cauda 30 (22,47-41,67) µm de comprimento, contento um filamento polar de 7 à 9 voltas. Análises morfológicas e filogenéticas permitiram a preposição de uma nova espécie, Henneguya sacacaensis n. sp, que pertence à família Myxobolidae e ao gênero Henneguya.

A note on the infection of Scomberomorus brasiliensis (Osteichthyes, Scombridae) by Kudoa sp. (Myxozoa: Multivalvulida) / Nota sobre a infeção de Scomberomorus brasiliensis (Osteichthyes, Scombridae) por Kudoa sp. (Myxozoa: Multivalvulida)

The infection of Scomberomorus brasiliensis by the myxozoan Kudoa sp. is reported. The parasites formed plasmodiae inside the skeletal muscle fibres. The spores were quadrate in apical view and bell-like shaped in lateral view, containing four equally sized more or less rounded polar capsules. No detrimental effects were observed in the host, namely muscle liquefaction. The importance of these parasites is discussed.

Relata-se a parasitose de Scomberomorus brasiliensis pelo myxozoa Kudoa sp. Os parasitas formavam plasmódios no interior das fibras musculares esqueléticas. Os esporos tinham forma quadrada em observação apical e forma de sino achatado em observação lateral, contendo quatro cápsulas polares do mesmo tamanho e sensivelmente arredondas. Não foram observadas lesões nos hospedeiros, nomeadamente liquefacção do músculo. A importância destes parasitas é discutida.

Ultrastructural description of Ceratomyxa microlepis sp. nov. (Phylum Myxozoa): a parasite infecting the gall bladder of Hemiodus microlepis, a freshwater teleost from the Amazon River

A new ceratomyxid parasite was examined for taxonomic identification, upon being found infecting the gall bladder of Hemiodus microlepis (Teleostei: Hemiodontidae), a freshwater teleost collected from the Amazon River, Brazil. Light and transmission electron microscopy revealed elongated crescent-shaped spores constituted by two asymmetrical shell valves united along a straight sutural line, each possessing a lateral projection. The spores body measured 5.2 ± 0.4 µm (n = 25) in length and 35.5 ± 0.9 µm (n = 25) in total thickness. The lateral projections were asymmetric, one measuring 18.1 ± 0.5 µm (n = 25) in thickness and the other measuring 17.5 ± 0.5 µm (n = 25) in thickness. Two equal-sized subspherical polar capsules measuring 2.2 ± 0.3 µm in diameter were located at the same level, each possessing a polar filament with 5-6 coils. The sporoplasm was binucleate. Considering the morphometric data analyzed from the microscopic observations, as well as the host species and its geographical location, this paper describes a new myxosporean species, herein named Ceratomyxa microlepis sp. nov.; therefore representing the first description of a freshwater ceratomyxid from the South American region.

Fine structure of Henneguya hemiodopsis sp. n. (Myxozoa), a parasite of the gills of the Brazilian teleostean fish Hemiodopsis microlepes (Hemiodontidae)

A fish-infecting myxosporean, Henneguya hemiodopsis sp. n., found infecting the gills of Hemiodopsis microlepis and collected from the Poty River near the city of Teresina, Brazil, was described based on ultrastructural studies. The parasite occurred within large whitish polysporic plasmodia (up to 200 ìm in diameter) containing asynchronous developmental sporogonic stages, mainly mature spores. The spores measured 19.7 ± 0.9 ìm in total length (n = 30) and the ellipsoidal spore body was 10.8 ± 0.5 ìm long, 3.3 ± 0.4 ìm wide and 2.5 ± 0.5 ìm thick. The spores were composed of two equal shell valves adhering together along the straight suture line, with each valve having equal-sized caudal tapering tails measuring 8.7 ± 0.6 ìm in length. The spores were surrounded by a thin anastomosed network of microfibrils, more evident on the tails. There were two symmetric elongated bottle-like polar capsules 3.5 ± 0.3 ìm long and 1.0 ± 0.2 ìm wide, each with a polar filament with five to six coils. Given the morphological and ultrastructural differences from previously described parasites and the specificity of the host species, we propose a new species, named H. hemiodopsis sp. n.