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1.
Rev. cuba. estomatol ; 44(3)jul.-sep. 2007. tab
Artículo en Español | LILACS, CUMED | ID: lil-498739

RESUMEN

La gingivitis crónica constituye una de las formas más frecuentes de enfermedad periodontal, caracterizada por la inflamación crónica de la encías, tumefacción, enrojecimiento y sangramiento. Su principal factor de riesgo lo constituye la microbiota del surco gingival, que resulta necesario, pero no suficiente para desencadenarla. Se realizó un ensayo clínico-terapéutico fase II, controlado, aleatorizado y a simple ciegas, para evaluar los efectos clínicos y microbiológicos del tratamiento combinado de la radiación láser helio-neón (He-Ne) con la clorhexidina al 0,2 por ciento. Todos los pacientes recibieron tratamiento inicial; al mes de finalizado este, se distribuyeron aleatoriamente en 2 grupos: un grupo estudio que recibió la combinación láser-neón y clorhexidina al 0,2 por ciento y otro grupo control que solo recibió clorhexidina al 0,2 por ciento. Se realizó una evaluación a los 15, 30 y 45 días, con criterios de eficacia clínicos y microbiológicos. Los resultados clínicos fueron satisfactorios en el grupo estudio con predominio de los morfotipos I, caracterizados por cocos gramnegativos y positivos, compatibles con un periodonto sano. Los eventos adversos detectados con esta terapéutica fueron mínimos, todos relacionados con la somnolencia(AU)


Chronic gingivitis is one of the most common periodontal diseases that is characterized by chronic inflammation, tumefaction, redness and bleeding. The main risk factor is gingival sulcus microbiota that is essential but not enough to unleash it. A phase II controlled randomized blind clinical/therapeutical assay was conducted to evaluate the clinical and microbiological effects of the combined treatment based on helium-neon laser (He-Ne) with 0.2 per cent chlorhexidine. All the patients were initially treated; after a month, they were randomly distributed into two groups, that is, the study group received a helium-neon laser plus 0.2 per cent chlorhexidine combination and the control group was treated with 0.2 per cent chlorhexidine only. They were evaluated at 15th, 30th and 45th days by using clinical and microbiological efficacy criteria. The clinical results were satisfactory in the study group where morphotypes I, characterized by Gram-negative and Gram-positive cocci and compatible with a healthy periodontium, prevailed . Adverse events were minimal, all of them related to somnolence(AU)


Asunto(s)
Humanos , Enfermedades Periodontales/etiología , Clorhexidina/uso terapéutico , Gingivitis/terapia , Helio/uso terapéutico , Rayos Láser/efectos adversos , Neón/uso terapéutico , Somnolencia
2.
Braz. j. med. biol. res ; 40(6): 877-884, June 2007. tab, ilus, graf
Artículo en Inglés | LILACS | ID: lil-452675

RESUMEN

We determined the effects of helium-neon (He-Ne) laser irradiation on wound healing dynamics in mice treated with steroidal and non-steroidal anti-inflammatory agents. Male albino mice, 28-32 g, were randomized into 6 groups of 6 animals each: control (C), He-Ne laser (L), dexamethasone (D), D + L, celecoxib (X), and X + L. D and X were injected im at doses of 5 and 22 mg/kg, respectively, 24 h before the experiment. A 1-cm long surgical wound was made with a scalpel on the abdomens of the mice. Animals from groups L, D + L and X + L were exposed to 4 J (cm²)-1 day-1 of He-Ne laser for 12 s and were sacrificed on days 1, 2, or 3 after the procedure, when skin samples were taken for histological examination. A significant increase of collagen synthesis was observed in group L compared with C (168 ± 20 vs 63 ± 8 mm²). The basal cellularity values on day 1 were: C = 763 ± 47, L = 1116 ± 85, D = 376 ± 24, D + L = 698 ± 31, X = 453 ± 29, X + L = 639 ± 32 U/mm². These data show that application of L increases while D and X decrease the inflammatory cellularity compared with C. They also show that L restores the diminished cellularity induced by the anti-inflammatory drugs. We suggest that He-Ne laser promotes collagen formation and restores the baseline cellularity after pharmacological inhibition, indicating new perspectives for laser therapy aiming to increase the healing process when anti-inflammatory drugs are used.


Asunto(s)
Animales , Masculino , Ratones , Antiinflamatorios/uso terapéutico , Dexametasona/uso terapéutico , Terapia por Luz de Baja Intensidad , Pirazoles/uso terapéutico , Sulfonamidas/uso terapéutico , Cicatrización de Heridas/efectos de la radiación , Helio/uso terapéutico , Neón/uso terapéutico , Cicatrización de Heridas/efectos de los fármacos
3.
Fisioter. pesqui ; 13(3): 31-35, set.-dez. 2006. ilus, tab
Artículo en Portugués | LILACS | ID: lil-450830

RESUMEN

O laser de baixa potência é utilizado na fisioterapia na cicatrização de lesões, para acelerar a reparação tecidual. Um filme de PVC na ponteira do equipamento é comumente usado na prática clínica para evitar a contaminação da lesão pelo equipamento, principalmente em mucosas e áreas cruentas...


Low-intensity laser application is used in physical therapy in view of accelerating wound repair processes. In clinical practice, a PVC film is commonly used covering the lasers pen's tip to avoid contamination of the wound by the equipment, mainly on mucosa and cruent...


Asunto(s)
Aluminio/uso terapéutico , Heridas y Lesiones/rehabilitación , Terapia por Luz de Baja Intensidad/métodos , Neón/uso terapéutico
4.
MJIRC-Medical Journal of the Iranian Red Crescent. 2005; 7 (3): 27-32
en Inglés | IMEMR | ID: emr-73681

RESUMEN

Reducing pain and enhancement of wound healing have several advantages. Wound healing is a complicated but regular process that includes synchronized and organized phenomena such as parenchymal cells migration, proliferation and synthesis of extra- cellular matrix proteins. Rate of wound healing is affected by various factors. The aim of our research was to study the effects of low power He-Ne laser radiation [632.8 nm] on metabolic process, cell proliferation and finally wound repairment. In our research about 50 healthy mature rats were studied by case-control method. After shaving, small cuts 10 mm in length were made at the neck. Then they were divided randomly into control and case groups. The rats of the case group were radiated by He-Ne laser to their wounds about four minutes each day. After a weak we did wound biopsy of both groups and all the specimens were studied microscopically. The data was analyzed by t-test. Findings showed that there were significant increase in the fibroblast and connective tissue fibers and also vascularisation in the case group compared with the control group. Wound healing in the case group was accelerated and wound diameter was smaller significantly. So low power He-Ne laser radiation accelerates wound healing process


Asunto(s)
Animales de Laboratorio , Piel , Rayos Láser , Terapia por Luz de Baja Intensidad , Helio , Neón , Ratas
5.
Indian J Exp Biol ; 2004 Sep; 42(9): 866-70
Artículo en Inglés | IMSEAR | ID: sea-55767

RESUMEN

Influence of low-power (632.8 nm, Helium-Neon, 13 J/cm2, three times a week) laser on 13-week immobilized articular cartilage was examined with rabbits knee model. Number of chondrocytes and depth of articular cartilage of experimental group were significantly higher than those of sham irradiated group. Surface morphology of sham-irradiated group had rough prominences, fibrillation and lacunae but surface morphology of experimental group had more similarities to control group than to sham irradiated group. There were marked differences between ultrastructure features of control group and experimental group in comparison with sham irradiated group. Low-power Helium-Neon laser irradiation on 13-week immobilized knee joints of rabbits neutrilized adverse effects of immobilization on articular cartilage.


Asunto(s)
Animales , Cartílago Articular/fisiología , Condrocitos/fisiología , Helio/química , Rayos Láser/efectos adversos , Masculino , Neón/química , Conejos , Regeneración/efectos de la radiación
6.
Papua New Guinea medical journal ; : 8-15, 2003.
Artículo en Inglés | WPRIM | ID: wpr-631550

RESUMEN

An audit of neonatal care at Modilon Hospital, Madang was performed using obstetric and neonatal data for the five years 1995-1999. The overall perinatal mortality rate (PNMR) was 51.1 per 1000 total births with an early neonatal mortality rate (ENNMR) of 12.7 and a stillbirth rate (SBR) of 38.5. 839 neonates aged 0-28 days were admitted to the Special Care Nursery. The male to female ratio was 1.3:1. 186 babies (22%) died. The case fatality rate was higher in males than females (p<0.001). Babies born at health centres or born before arrival had a significantly higher fatality rate than hospital-born babies (p<0.001). The case fatality rate was highest in babies born preterm and declined with increasing birthweight from less than 1000 to 3999 g. The major recorded causes of admission were neonatal sepsis, prematurity, neonatal jaundice, birth asphyxia, respiratory distress and meconium aspiration syndrome. 60% of deaths occurred within 48 hours of admission, 32% between 48 hours and 7 days and 8% at 7 days or older. The proportion of deaths occurring during the afternoon and night shifts was significantly higher than that during the morning shift (p<0.001). This was most likely to be related to staffing levels. The major causes of death were prematurity or low birthweight (27%), sepsis (23%) and birth asphyxia (17%). Other causes of death included congenital abnormalities, meconium aspiration and meningitis. Antenatal care is still not universally available for Papua New Guinean women. Home delivery of high-risk mothers is commonplace, and women delivering in hospital often present in established labour. Perinatal and neonatal problems are therefore frequent. Newborn babies have the right to the best available care. This can only be provided if hospitals and health facilities understand the basic requirements of neonatal care and provide designated space, adequate staffing and proper equipment.


Asunto(s)
Neón , Hospitales
7.
Rev. cuba. med. mil ; 31(1): ERRO-01_5-2, ene.-mar. 2002. tab
Artículo en Español | LILACS | ID: lil-327159

RESUMEN

Al conocer el estado inmunológico de los pacientes traumatizados según el índice predictivo TRISS, en quienes se ha demostrado una depresión de la inmunidad cuando la probabilidad de sobrevida es < 95 porciento y conociendo además la acción del láser blando o de baja potencia con su efecto inmunomodulador a escala celular, antiinflamatorio y cicatrizante, se realizó un ensayo clínico piloto prospectivo, descriptivo y longitudinal en 40 traumatizados con probabilidad de sobrevida < 95 porciento que ingresaron en el Instituto Superior de Medicina Militar "Dr. Luis Díaz Soto" en un período de 8 meses, con el fin de evaluar la utilidad de la terapia láser utilizando puntos acupunturales de acción inmunoestimulante a un régimen de una sesión diaria por 10 días, a los cuales también se les realizaron estudios de leucocitos y plaquetas. La probabilidad de sobrevida promedio fue del 89,7 porciento. El 15 porciento de los lesionados sufrieron algún tipo de complicación séptica y falleció solo 1 paciente para el 2,5 porciento. Al décimo día de tratamiento se obtuvieron valores más elevados en cuanto a conteo de leucocitos y plaquetas con respecto al primer día de tratamiento. Por los resultados obtenidos se concluyó que la terapia laseracupuntura con helio-neón con propiedad inmunomoduladora es útil cuando se utiliza oportunamente en pacientes traumatizados con TRISS < 95 porciento, con lo que se logra disminuir la incidencia de complicaciones y fallecimientos


Asunto(s)
Acupuntura , Helio , Neón , Rayos Láser/uso terapéutico , Heridas y Lesiones
8.
Chinese Journal of Traumatology ; (6): 246-249, 2002.
Artículo en Inglés | WPRIM | ID: wpr-332958

RESUMEN

<p><b>OBJECTIVE</b>To explore the inhibitory effect of He-Ne laser irradiation on fibroblast growth of hypertrophic scars in culture.</p><p><b>METHODS</b>He-Ne laser with wavelength of 632.8 nm, power density of 50 mW/cm(2) and doses of 3 J/cm(2), 30 J/cm(2), 90 J/cm(2) and 180 J/cm(2) was used to irradiate human scar fibroblasts in culture 1, 3 and 5 times respectively, and then the cell count and cell cycle analysis were done.</p><p><b>RESULTS</b>Repeated irradiation with He-Ne laser at dose of 180 J/cm(2) three and five times led to an evident decrease in total cell number compared with that of the control group and there was a significant difference (P<0.05). The cell cycle analysis showed after three and five times of irradiation with 180 J/cm(2) He-Ne laser the cell number in S-phase decreased from 51% to 20% and 14% respectively, the cell number in G(0)/G(1) phase increased from 28% to 55% and 60% respectively, and the cell percentage in Sub-G1 phase was 6.7% and 9.8% respectively.</p><p><b>CONCLUSIONS</b>Repeated irradiation with 180 J/cm(2) He-Ne laser can inhibit scar fibroblasts growth in culture. It may be that He-Ne laser irradiation causes cell stagnation in G(0)/G(1) phase and apoptosis.</p>


Asunto(s)
Femenino , Humanos , Masculino , División Celular , Efectos de la Radiación , Células Cultivadas , Cicatriz , Patología , Relación Dosis-Respuesta en la Radiación , Fibroblastos , Biología Celular , Efectos de la Radiación , Helio , Rayos Láser , Neón
9.
Papua New Guinea medical journal ; : 185-96, 2002.
Artículo en Inglés | WPRIM | ID: wpr-631624

RESUMEN

From June 1998 to December 1999, mothers of 150 babies who died in the early neonatal period and 150 controls whose babies did not die were studied. In multiple logistic regression analysis the following variables were positively associated with early neonatal deaths: lack of antenatal attendance, thick meconium staining of the liquor, male sex, very low birthweight and delivery at gestational age less than 34 weeks. Maternal betelnut chewing was negatively associated with neonatal deaths. When babies with birthweight below 1000 g were excluded, the following variables were associated with early neonatal deaths: unmarried status, thick meconium staining of the liquor and gestational age below 34 weeks. The negative association with betelnut chewing persisted. The main causes of early neonatal deaths were respiratory distress syndrome, septicaemia, birth asphyxia, meconium aspiration syndrome and congenital abnormalities. Avoidable factors in these deaths were associated with the patient (53%), the labour ward (28%), the antenatal clinic (9%), the postnatal ward (8%) and the special care nursery (2%).


Asunto(s)
Neón , Meconio
10.
Acta odontol. venez ; 38(1): 61-68, 2000. ilus, tab
Artículo en Español | LILACS | ID: lil-304759

RESUMEN

Con este artículo se pretende dar comienzo a una serie de publicaciones para así plasmar todo lo relacionado con el uso de la tecnología Laser en la odontología restauradora. En este primer artículo leerá sobre los antecedentes y fundamentos físicos del rayo laser, la clasificación y tipos de laser disponibles para área de la odontología, su interrelación con los tejidos vivos, así como las precauciones a tomar durante su uso. En las siguientes publicaciones de esta serie leeremos las particularidades de cada tipo de rayo laser que es utilizado en la odontología restauradora


Asunto(s)
Rayos Láser/clasificación , Restauración Dental Permanente/instrumentación , Aluminio , Argón , Cicatrización de Heridas/efectos de la radiación , Esmalte Dental , Dentina , Dióxido de Carbono/química , Seguridad de Equipos , Erbio , Helio , Holmio , Neodimio , Neón , Fibras Ópticas , Rayos Láser/normas , Rayos Láser/uso terapéutico , Dispersión de Radiación , Itrio
11.
Rev. mex. ortop. traumatol ; 13(5): 455-8, sept.-oct. 1999. tab, ilus
Artículo en Español | LILACS | ID: lil-276511

RESUMEN

El objetivo del trabajo fue determinar las condiciones óptimas para una aceleración de la cicatrización en piel de conejos mediante el tratamiento de la herida con laser de helio-neón HeNe (632.8 nm). Para este fin se usaron dos grupos de 5 conejos cada uno, en uno se aplicó el tratamiento con laser y el otro sirvió de control. A los animales se les realizó un resección de piel de 2 x 5 cm. El grupo experimental se irradió con laser helio-neón con una potencia de 0.1 mW a una distancia aproximada de 3 cm, con un barrido en toda la herida, por un lapso de 5 min/día. Se tomó biopsia después de las 24 horas al primer conejo, posteriormente, cada 24 horas se tomó un conejo distinto y se realizó el análisis histológico. En el grupo experimental se observó un aumento en la actividad de los procesos de reparación, en comparación con el grupo control, como fue la proliferación de fibroblastos y el incremento en la fibras de colágena que sellaron la herida a partir del cuarto día. En las condiciones antes mencionadas se redujo el tiempo de cicatrización, por lo menos en un 40 por ciento comparado con el grupo control


Asunto(s)
Animales , Conejos , Helio/uso terapéutico , Rayos Láser/uso terapéutico , Neón/uso terapéutico , Cicatrización de Heridas/efectos de la radiación , Modelos Biológicos , Conejos/cirugía , Proyectos de Investigación
13.
Korean Journal of Dermatology ; : 279-288, 1996.
Artículo en Coreano | WPRIM | ID: wpr-142168

RESUMEN

BACKGROUND: Recently it has been suggested that lasers can modulate the biological functions of cells in vitro. It has also been reported that a helium-neon(He-Ne) laser can stimulate wound healing in the absence of thermal effects. However, the results of more recent studies on the influence of He-Ne laser irradiation on the proliferation and collagen synthesis of human dermal fibroblasts have been inconclusive. And most experiments have not been performed in a three-dimensional collagen lattice that is the physiologic model for an in vitro experiment. OBJECTIVE: In this study we have investigated the nature of the influence of He-Ne laser irradiation on the proliferatior and collagen synthesis of human dermal fibroblasts cultured in a monolayer and collagen lattice. METHODS: We used a 10mW He-Ne laser emitting a beam of wavelength 632,8nm. The human dermal fibroblasts were subjected to laser treatment at various energy densities, and the treatment schedule included one daily exposure on three consecutive days. DNA replication was assessed by H-thymidine incorporation, and the collagen production was monitored by the synthesis of H-hydroxyproline following incubation of the culture with H-proline. RESULTS: The results were as follows : In the control group, the proliferation and collagen synthesis of fibroblasts sultured in a collagen lattice on day 4 and 7 were significantly reduced compared with conventional manolayer cultures. Although the proliferation of human dermal fibroblasts was not remarkable in all experimental irradiation energy except 4 J/cm in a monolayer culture, a statistically significant stimulating effect on fibroblasts proliferation in collagen lattice were noted on days 4 and 7 with an energy density of 1.5J/cm. And we also found that a great er increase of collagen synthesis of human dermal fibroblasts occurred in a monolayer culture on days 4 and 7 in all irradiated energy densities than those in a collagen lattice culture. But statistically significant enhancement of collagen synthesis was showed only at the energy density of 1 J/cm in a collagen lattice culture on days 4. CONCLUSION: These data indicated that the influence of He-Ne laser irradiation on the proliferation and collagen synthesis of human dermal fibroblasts cultured in a collagen lattice was remark ably different from those in a monolayer culture. In the clinical application of the He-Ne laser, the control of the amount of irradiation of the He Ne laser may regulate the proliferative activity and collagen synthesis of human dermal fibroblasts in wound healing.


Asunto(s)
Humanos , Citas y Horarios , Colágeno , Replicación del ADN , Fibroblastos , Helio , Neón , Cicatrización de Heridas
14.
Korean Journal of Dermatology ; : 279-288, 1996.
Artículo en Coreano | WPRIM | ID: wpr-142166

RESUMEN

BACKGROUND: Recently it has been suggested that lasers can modulate the biological functions of cells in vitro. It has also been reported that a helium-neon(He-Ne) laser can stimulate wound healing in the absence of thermal effects. However, the results of more recent studies on the influence of He-Ne laser irradiation on the proliferation and collagen synthesis of human dermal fibroblasts have been inconclusive. And most experiments have not been performed in a three-dimensional collagen lattice that is the physiologic model for an in vitro experiment. OBJECITVE: In this study we have investigated the nature of the influence of He-Ne laser irradiation on the proliferatior and collagen synthesis of human dermal fibroblasts cultured in a monolayer and collagen lattice. METHODS: We used a 10mW He-Ne laser emitting a beam of wavelength 632,8nm. The human dermal fibroblasts were subjected to laser treatment at various energy densities, and the treatment schedule included one daily exposure on three consecutive days. DNA replication was assessed by H-thymidine incorporation, and the collagen production was monitored by the synthesis of H-hydroxyproline following incubation of the culture with H-proline. RESULTS: The results were as follows : In the control group, the proliferation and collagen synthesis of fibroblasts sultured in a collagen lattice on day 4 and 7 were significantly reduced compared with conventional manolayer cultures. Although the proliferation of human dermal fibroblasts was not remarkable in all experimental irradiation energy except 4 J/cm in a monolayer culture, a statistically significant stimulating effect on fibroblasts proliferation in collagen lattice were noted on days 4 and 7 with an energy density of 1.5J/cm. And we also found that a great er increase of collagen synthesis of human dermal fibroblasts occurred in a monolayer culture on days 4 and 7 in all irradiated energy densities than those in a collagen lattice culture. But statistically significant enhancement of collagen synthesis was showed only at the energy density of 1 J/cm in a collagen lattice culture on days 4. CONCLUSION: These data indicated that the influence of He-Ne laser irradiation on the proliferation and collagen synthesis of human dermal fibroblasts cultured in a collagen lattice was remark ably different from those in a monolayer culture. In the clinical application of the He-Ne laser, the control of the amount of irradiation of the He Ne laser may regulate the proliferative activity and collagen synthesis of human dermal fibroblasts in wound healing.


Asunto(s)
Humanos , Citas y Horarios , Colágeno , Replicación del ADN , Fibroblastos , Helio , Neón , Cicatrización de Heridas
15.
Korean Journal of Dermatology ; : 279-288, 1996.
Artículo en Coreano | WPRIM | ID: wpr-142165

RESUMEN

BACKGROUND: Recently it has been suggested that lasers can modulate the biological functions of cells in vitro. It has also been reported that a helium-neon(He-Ne) laser can stimulate wound healing in the absence of thermal effects. However, the results of more recent studies on the influence of He-Ne laser irradiation on the proliferation and collagen synthesis of human dermal fibroblasts have been inconclusive. And most experiments have not been performed in a three-dimensional collagen lattice that is the physiologic model for an in vitro experiment. OBJECTIVE: In this study we have investigated the nature of the influence of He-Ne laser irradiation on the proliferatior and collagen synthesis of human dermal fibroblasts cultured in a monolayer and collagen lattice. METHODS: We used a 10mW He-Ne laser emitting a beam of wavelength 632,8nm. The human dermal fibroblasts were subjected to laser treatment at various energy densities, and the treatment schedule included one daily exposure on three consecutive days. DNA replication was assessed by H-thymidine incorporation, and the collagen production was monitored by the synthesis of H-hydroxyproline following incubation of the culture with H-proline. RESULTS: The results were as follows : In the control group, the proliferation and collagen synthesis of fibroblasts sultured in a collagen lattice on day 4 and 7 were significantly reduced compared with conventional manolayer cultures. Although the proliferation of human dermal fibroblasts was not remarkable in all experimental irradiation energy except 4 J/cm in a monolayer culture, a statistically significant stimulating effect on fibroblasts proliferation in collagen lattice were noted on days 4 and 7 with an energy density of 1.5J/cm. And we also found that a great er increase of collagen synthesis of human dermal fibroblasts occurred in a monolayer culture on days 4 and 7 in all irradiated energy densities than those in a collagen lattice culture. But statistically significant enhancement of collagen synthesis was showed only at the energy density of 1 J/cm in a collagen lattice culture on days 4. CONCLUSION: These data indicated that the influence of He-Ne laser irradiation on the proliferation and collagen synthesis of human dermal fibroblasts cultured in a collagen lattice was remark ably different from those in a monolayer culture. In the clinical application of the He-Ne laser, the control of the amount of irradiation of the He Ne laser may regulate the proliferative activity and collagen synthesis of human dermal fibroblasts in wound healing.


Asunto(s)
Humanos , Citas y Horarios , Colágeno , Replicación del ADN , Fibroblastos , Helio , Neón , Cicatrización de Heridas
16.
Korean Journal of Dermatology ; : 279-288, 1996.
Artículo en Coreano | WPRIM | ID: wpr-142163

RESUMEN

BACKGROUND: Recently it has been suggested that lasers can modulate the biological functions of cells in vitro. It has also been reported that a helium-neon(He-Ne) laser can stimulate wound healing in the absence of thermal effects. However, the results of more recent studies on the influence of He-Ne laser irradiation on the proliferation and collagen synthesis of human dermal fibroblasts have been inconclusive. And most experiments have not been performed in a three-dimensional collagen lattice that is the physiologic model for an in vitro experiment. OBJECITVE: In this study we have investigated the nature of the influence of He-Ne laser irradiation on the proliferatior and collagen synthesis of human dermal fibroblasts cultured in a monolayer and collagen lattice. METHODS: We used a 10mW He-Ne laser emitting a beam of wavelength 632,8nm. The human dermal fibroblasts were subjected to laser treatment at various energy densities, and the treatment schedule included one daily exposure on three consecutive days. DNA replication was assessed by H-thymidine incorporation, and the collagen production was monitored by the synthesis of H-hydroxyproline following incubation of the culture with H-proline. RESULTS: The results were as follows : In the control group, the proliferation and collagen synthesis of fibroblasts sultured in a collagen lattice on day 4 and 7 were significantly reduced compared with conventional manolayer cultures. Although the proliferation of human dermal fibroblasts was not remarkable in all experimental irradiation energy except 4 J/cm in a monolayer culture, a statistically significant stimulating effect on fibroblasts proliferation in collagen lattice were noted on days 4 and 7 with an energy density of 1.5J/cm. And we also found that a great er increase of collagen synthesis of human dermal fibroblasts occurred in a monolayer culture on days 4 and 7 in all irradiated energy densities than those in a collagen lattice culture. But statistically significant enhancement of collagen synthesis was showed only at the energy density of 1 J/cm in a collagen lattice culture on days 4. CONCLUSION: These data indicated that the influence of He-Ne laser irradiation on the proliferation and collagen synthesis of human dermal fibroblasts cultured in a collagen lattice was remark ably different from those in a monolayer culture. In the clinical application of the He-Ne laser, the control of the amount of irradiation of the He Ne laser may regulate the proliferative activity and collagen synthesis of human dermal fibroblasts in wound healing.


Asunto(s)
Humanos , Citas y Horarios , Colágeno , Replicación del ADN , Fibroblastos , Helio , Neón , Cicatrización de Heridas
17.
Rev. cuba. ortop. traumatol ; 7(1/2): 81-7, ene.-dic. 1993. tab
Artículo en Español | LILACS | ID: lil-150002

RESUMEN

Se estudiaron 40 pacientes de uno y otro sexos con artritis reumatoide, toma articular y vírgenes de tratamiento, diagnosticados en el Instituto de Reumatología. Se aplicó laserterapia combinada helio-neón e infrarrojo en barrido y focal en 2 ciclos de 20 sesiones cada uno, separados por un período de descanso de un mes. Los pacientes se evaluaron antes del tratamiento y después de este mediante criterios clínicos (inflamación articular con cinta métrica o anillo de joyero, amplitud articular con goniómetro, tiempo de marcha -15 metros-minutos-, fuerza de puño, dolor mediante escala visual del dolor y rigidez matinal) y humorales (velocidad de eritrosedimentación, proteína C-reactiva y factor reumatoideo). Además al final del tratamiento se aplicó la escala de Long-Range (capacidad para la independencia). Se obtuvo una mejoría significativa (p<0,05) con el tratamiento lo que permitió a los pacienres mejorar tanto su disposición como su capacidad para las actividades de la vida diaria, social y laboral


Asunto(s)
Humanos , Artritis Reumatoide/radioterapia , Rayos Láser/uso terapéutico , Helio/uso terapéutico , Rayos Infrarrojos/uso terapéutico , Neón/uso terapéutico
18.
Dermatol. rev. mex ; 36(2): 104-7, mar.-abr. 1992. ilus
Artículo en Español | LILACS | ID: lil-118200

RESUMEN

Se presenta un paciente masculino de 30 años de edad, que sufrió en el dorso del pie derecho una picadura de animal marino (raya) cuando cruzaba la desembocadura de un río. No se procedió de inmediato a realizar las medidas específicas para neutralizar la acción proteolítica de la sustancia inoculada; 48 horas después del accidente presentó edema del pie derecho y fiebre. A la semana siguiente presentaba una úlcera necrótica en el sitio de inoculación con un trayecto fistuloso que llegaba hasta las partes blandas de la región plantar. El paciente fue tratado con laser gaseoso de Helio-Neón obteniendo la curación total sin recidivas a las 20 sesiones.


Asunto(s)
Humanos , Animales , Masculino , Adulto , Mordeduras y Picaduras/complicaciones , Enfermedades del Pie/radioterapia , Rayos Láser/uso terapéutico , Rajidae , Úlcera Cutánea/radioterapia , Cuba , Enfermedades del Pie/etiología , Enfermedades del Pie/patología , Helio/uso terapéutico , Neón/uso terapéutico , Úlcera Cutánea/etiología , Úlcera Cutánea/patología
20.
Rev. cuba. estomatol ; 27(4): 423-9, oct.-dic. 1990. tab
Artículo en Español | LILACS | ID: lil-112126

RESUMEN

Se presenta un estudio en 30 pacientes con diagnóstico de síndrome dolor disfunción temporomandibular de causa articular que fueron organizados en 3 grupos, según el tratamiento recibido. Grupo A: tratamiento con medicamentos ; grupo B :tratamiento con radiación láser helioneón: Grupo C: tratamiento combinado de medicamentos y radiación láser helioneón. Se comparan los resultados obtenidos en cada grupo de estudio; se observa mayor efectividad y permanencia del efecto analgésico logrado con la radiación láser helioneón y la combinación de fármacos con helioneón


Asunto(s)
Humanos , Helioterapia , Rayos Láser/uso terapéutico , Neón/uso terapéutico , Síndrome de la Disfunción de Articulación Temporomandibular/tratamiento farmacológico , Neón/uso terapéutico , Síndrome de la Disfunción de Articulación Temporomandibular/radioterapia
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