1.
Indian J Exp Biol
; 2001 Jul; 39(7): 650-3
Artículo
en Inglés
| IMSEAR
| ID: sea-57161
RESUMEN
An heterologous transformation system for entomopathogenic fungi B. bassiana and M. anisopliae was developed based on the use of A. nidulans nitrate reductase gene (niaD). B. bassiana and M. anisopliae niaD stable mutants were selected by treatment of protoplast with ethane methane sulphonate (EMS) and regenerated on chlorate medium. The cloned gene was capable of transforming B. bassiana and M. anisopliae at a frequency of 5.8 to 20 transformants per microg of DNA. Most of them were mitotically stable.
Asunto(s)
Aspergillus niger/enzimología , Genes Fúngicos , Hypocreales/citología , Mitosis , Mutación , Nitrato-Reductasa , Nitrato Reductasas/genética , Transformación Genética
2.
Indian J Exp Biol
; 1980 Oct; 18(10): 1165-7
Artículo
en Inglés
| IMSEAR
| ID: sea-58744