1.
Indian J Biochem Biophys
; 2008 Oct; 45(5): 350-3
Artículo
en Inglés
| IMSEAR
| ID: sea-26600
RESUMEN
The kinetics of alpha-chymotrypsin (alpha-CT) catalyzed hydrolysis of 4-nitrophenyl acetate has been studied in aqueous solution of alkyldimethylethanolammonium bromide (cetyl, dodecyl, decyl) surfactants at concentrations below and above their critical micelle concentration. From Michaelis-Mcnten kinetics, the catalytic rate constant kcat and the Michaelis constant KM have been determined. The bell-shaped profiles of alpha-CT activity with increasing surfactant concentrations indicate the interaction between the micelle-bound enzyme and substrate.
Asunto(s)
Biocatálisis , Quimotripsina/metabolismo , Etanolamina/química , Hidrólisis , Cinética , Nitrofenoles/metabolismo , Tensoactivos/química
2.
Indian J Biochem Biophys
; 1977 Jun; 14(2): 188-91
Artículo
en Inglés
| IMSEAR
| ID: sea-28590