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1.
Pesqui. bras. odontopediatria clín. integr ; 23: e220116, 2023. tab, graf
Artículo en Inglés | LILACS, BBO | ID: biblio-1448801

RESUMEN

ABSTRACT Objective: To investigate the effects of orthodontic tooth movement on clinical attachment level (CAL) changes in treated periodontitis in adult patients with malocclusion. Material and Methods: Present study is based on PRISMA guidelines; all articles published in international databases such as PubMed, Scopus, Science Direct, and Embase between 2012 to May 2022 are included. 95% confidence interval (CI) for mean difference with fixed effect modal and inverse-variance were calculated. Data analysis was performed using STATA.V16 software. Results: In the initial review, duplicate studies were eliminated, abstracts of 175 studies were reviewed, two authors reviewed the full text of 21 studies, and finally, eleven studies were selected. The mean of CAL gain was 2.29 mm (MD, 95% CI -2.47 mm, -2.12 mm; p=0.00) (I2=91.81%; p=0.00; high heterogeneity). The mean difference of PPD changes was -1.93 mm (MD, 95% CI -2.07 mm, -1.80 mm; p=0.00) (I2=98.52%; p=0.00; high heterogeneity). Conclusion: Due to the limitations of the study and based on the meta-analysis, it is observed that orthodontic treatment is performed with higher success after reconstructive surgery with periodontal improvement.


Asunto(s)
Humanos , Masculino , Femenino , Adulto , Periodontitis/patología , Técnicas de Movimiento Dental , Maloclusión , Enfermedades Periodontales/patología , Interpretación Estadística de Datos
2.
Artículo en Inglés | LILACS, BBO | ID: biblio-1180862

RESUMEN

ABSTRACT Objective: To identify etiologic microbiota associated periodontal diseases among diabetes patients and the factors related to the most commonly identified bacteria species. Material and Methods: Periodontal plaque samples from 11 diabetic participants and 13 non-diabetic controls were collected to assess their aerobic and anaerobic bacterial growth. Different distinct colonies were identified by microscopic and 16srDNA sequencing. Pearson's chi-square tests were conducted to examine any association between categorical variables. Results: The diabetic subjects revealed a more intense plaque formation with a mean plaque index of 2.4 compared to 1.8 in non-diabetics. A total of 86 bacteria were isolated from 24 plaque samples, 44 were aerobic, and 42 were anaerobic. Only aerobic isolates, 22 from diabetic patients and 22 from non-diabetic patients, were evaluated in these analyses. Bacillus spp. (B. cereus mainly) and Klebsiella spp. (K. pneumoniae, K. aerogenes, K. oxytoca) were detected markedly higher in non-diabetic individuals than in diabetic subjects (p=0.026 and p=0.021, respectively). Some bacteria were only identified in the dental plaque of diabetic individuals, namely, Bacillus mojavensis, Enterobacter cloacae, Proteus mirabilis, Staphylococcus epidermidis, Staphylococcus hominis, Staphylococcus pasteuri, Streptococcus mutans, and Streptococcus pasteurianus. The presence of acid reflux and jaundice were significantly associated with the most common bacterial isolate, namely Bacillus spp., with the p-values of 0.007 and 0.001, respectively. Conclusion: Type-2 diabetes mellitus is associated with a higher amount of dental plaques. Periodontal plaque samples from diabetic and non-diabetic subjects possess differential microbial communities. Diabetic plaques contain more versatile microbes predominated by gram-positive streptococci and staphylococci.


Asunto(s)
Humanos , Masculino , Femenino , Niño , Adolescente , Adulto , Persona de Mediana Edad , Anciano , Enfermedades Periodontales/etiología , Periodontitis/patología , Salud Bucal/educación , Diabetes Mellitus Tipo 2/microbiología , Microbiota/inmunología , Streptococcus mutans/inmunología , Bangladesh/epidemiología , Radiografía Dental/instrumentación , Distribución de Chi-Cuadrado , Atención Odontológica , Placa Dental , Diabetes Mellitus/microbiología
3.
Artículo en Inglés | LILACS, BBO | ID: biblio-1250459

RESUMEN

Abstract Objective: To measure the level of immunoglobulin G (IgG) in the gingival crevicular fluid (GCF). Material and Methods: A total of 158 patients aged >45 years were examined for periodontitis and interviewed regarding their menopausal status. The non-menopause group entailed female patients with periodontitis without menopause (n=23). The menopause group included females who stopped menstruating since >1 year, had a pocket depth of 4-5 mm, and did not have other systemic conditions (n=40). Samples were selected based on periodontal and menopausal status. In total, 63 samples of GCF were collected from the participants and tested using an enzyme-linked immunosorbent assay kit for IgG. Results: The median level of IgG in the menopause group was 39.50 (g/mL, whereas that of the non-menopause group was 41.08 (g/mL. There was a positive correlation between the plaque index and IgG level in both groups. In contrast, there was a negative correlation between age and IgG level. However, there was no correlation between plaque index and age regarding the IgG level in both groups (p>0.05). Conclusion: The IgG levels in the menopause group were lower than those in the non-menopause group. As such, menopausal females should take great care of their overall health, including the periodontium.


Asunto(s)
Humanos , Femenino , Adulto , Persona de Mediana Edad , Enfermedades Periodontales/patología , Periodontitis/patología , Periodoncio , Inmunoglobulina G , Menopausia , Estudios Transversales/métodos , Líquido del Surco Gingival , Estadísticas no Paramétricas , Indonesia/epidemiología
4.
West China Journal of Stomatology ; (6): 518-523, 2021.
Artículo en Inglés | WPRIM | ID: wpr-921368

RESUMEN

OBJECTIVES@#To investigate the effect of peroxisome proliferator-activated receptor-γ coactivator-1α (PGC-1α) on liver injury induced by periodontitis in rats.@*METHODS@#Twenty-four male Wistar rats were randomly divided into two groups: control group and periodontitis group, twelve per group. In periodontitis group, the periodontitis models were established for the maxillary first molars in rats by means of "wire ligation+vaccinationwith @*RESULTS@#The probing depth, tooth mobility and sulcus bleeding index in periodontitis group were significantly higher than that in control group. HE staining showed in periodontitis group, hepatic cords ranged disorderly and there were vacuoles in cells and inflammatory cells infiltrated in liver tissues of rats, and there was no obvious abnormality in control group. The qRT-PCR results showed that the mRNA expression levels of @*CONCLUSIONS@#PGC-1α may be involved in the process of periodontitis-induced liver injury in rats.


Asunto(s)
Animales , Masculino , Ratas , Hígado/lesiones , PPAR gamma , Periodontitis/patología , Ratas Wistar
5.
Artículo en Inglés | LILACS, BBO | ID: biblio-1154998

RESUMEN

ABSTRACT Objective: To evaluate the effects of commonly used smokeless tobacco forms on oral health at habitual placement sites of smokeless tobacco compared to non-placement sites among the North Indian population. Material and Methods: This cross-sectional study was conducted among 542 individuals using smokeless tobacco recruited from the outpatient wing of the Dental College. Subjects completed a questionnaire and received an oral examination. Periodontal pocket depth, gingival index, plaque index, gingival recession, and oral mucosal changes were assessed. Kendal's Tau test, paired t-test, and chi-square test were carried out to compare different variables among placement and non-placement sites. Results: Most of the subjects were male, reporting an average of 11.26 years of SLT use. Clinical inflammation of gingiva was significantly greater (p=0.01) at placement-sites (1.64 ± 0.53) of SLT in comparison to non-placement-sites (1.40 ± 0.41). The difference in the GR and PPD at placement and non-placement-sites was also statistically significant with p=0.002 and p=0.001, respectively. Clinically, the majority of subjects had mucosal changes at the placement sites, and a statistically significant association (p=0.034) was observed between the duration of the use of smokeless tobacco and the mucosal changes. Conclusion: Smokeless tobacco use predisposes to increased risk of periodontal diseases and oral mucosal changes at the placement sites in an individual due to the local irritant effect.


Asunto(s)
Humanos , Masculino , Femenino , Adolescente , Adulto , Persona de Mediana Edad , Periodontitis/patología , Salud Bucal/educación , Tabaco sin Humo/toxicidad , India/epidemiología , Mucosa Bucal/lesiones , Distribución de Chi-Cuadrado , Epidemiología Descriptiva , Estudios Transversales , Encuestas y Cuestionarios
6.
Artículo en Inglés | LILACS, BBO | ID: biblio-1287499

RESUMEN

ABSTRACT Objective: To analyze caries experience depending on periodontitis affection concomitant or not with cardiovascular risks or mental health issues with the hypothesis that worse accumulated caries experience is not necessarily the result of the presence of concomitant overall health issues. Material and Methods: All subjects of this cross-sectional study were participants of the Dental Registry and DNA Repository project. Variables age, ethnicity, sex, caries experience, periodontitis and mental disease statuses and blood pressure assessment, dental clinical data (DMFT, DMFS and periodontitis) were evaluated and collected from the clinical records. We used chi-square, Fisher's exact, or Student's t-tests to determine differences in frequencies of sex, age, ethnicity, and dental clinical data depending on caries experience, periodontitis, mental health status, and cardiovascular risks. The established alpha was 5%. Results: Of the total 1,437 subjects included in this study, 407 were individuals with high blood pressure and 1,030 were individuals without high blood pressure. Also, 558 were individuals with mental disease and 879 were individuals without mental disease. High blood pressure patients were mostly men (47.17%) and women were the majority when analyzing patients with mental illness (60.04%). Most of the patients in the different groups were white, with a mean age ranging from 15.4 to 88 years. Conclusion: We concluded that caries experience concomitant or not with periodontitis affection does not associate with high blood pressure and mental disease.


Asunto(s)
Humanos , Masculino , Femenino , Adolescente , Adulto , Persona de Mediana Edad , Anciano , Anciano de 80 o más Años , Periodontitis/patología , Salud Bucal/educación , Caries Dental/prevención & control , Trastornos Mentales/psicología , Brasil/epidemiología , Distribución de Chi-Cuadrado , Índice CPO , Registros Médicos , Estudios Transversales/métodos , Interpretación Estadística de Datos
7.
Acta odontol. latinoam ; 33(1): 50-55, June 2020. graf
Artículo en Inglés | LILACS | ID: biblio-1130733

RESUMEN

ABSTRACT The purpose of this study was to evaluate aortic wall thickness after periodontal disease and/or obesity induction in a Wistar rat model. Sixty male Wistar rats were randomly divided into four groups: control (CT), periodontal disease (PD), obesity (OB), and obesity plus periodontal disease (OB+PD). Groups OB and OB+PD received cafeteria diet for 17 weeks. After they had acquired obesity (week 12), periodontal disease was induced by placing a silk ligature on the maxillary right second molar of groups PD and OB+PD. During the experimental period, body weight and Lee index were assessed. Mean alveolar bone loss (ABL) was evaluated, and aortas were prepared for histometric analysis of the aortic wall by ImageJ software. Body weight and Lee index increased in rats exposed to cafeteria diet. Mean ABL was higher in Groups PD and OB+PD than in control and OB (p<0.05). ABL was 18% higher in Group OB+PD than in Group PD, with statistically significant difference (p<0.001). Aortas were thicker in Groups OB and OB+PD than in control and PD groups, respectively (2.31mm ± 0.28 and 2.33 ± 0.29 vs. 2.18 ± 0.26 and 2.14 ± 0.27). Group OB differed significantly from the control group (p=0.036), and OB+PD and OB differed significantly from PD (p=0.004 and p= 0.001, respectively). Obesity alters aortic wall thickness in Wistar rats. However, the presence of periodontal disease did not affect the aortic wall thickness under the conditions of the present study.


RESUMO O objetivo deste estudo foi avaliar a espessura da parede da aorta após modelos de indução de doença periodontal e/ou obesidade em ratos Wistar. Sessenta ratos Wistar machos foram aleatoria mente divididos em quatro grupos: controle (CT), doença periodontal (DP), obesidade (OB), obesidade mais doença periodontal (OB+DP). Os grupos OB e OB+DP rece beram dieta de cafeteria por 17 semanas. Após de adquirirem obesidade, (semana 12), doença periodontal foi induzido pela colocação de ligaduras de seda no segundo molar superior direito dos grupos DP e OB+DP. Durante o período experi mental, o peso corporal e índice de Lee foram obtidos. Média de perda óssea alveolar (POA) foi avaliada e as aortas preparadas para análise histométrica da parede aórtica (em mm) pelo software ImageJ. Ratos expostos a dieta de cafeteria demonstraram um aumento do peso corporal e do índice de Lee. Uma POA media maior foi observada nos grupos DP e OB+DP comparado aos grupos controle e OB (p<0.05). O grupo OB+DP, quando comparado ao grupo DP, apresentou POA 18% maior e essa diferença foi estatisticamente significativa (p<0.001). Os grupos OB e OB+DP exibiram uma espessura de aorta maior comparado aos grupos DP e controle, respectivamente (2.31 ± 0.28 e 2.33 ± 0.29 vs. 2.18 ± 0.26 e 2.14 ± 0.27). Diferenças significativas foram observadas nas comparações dos grupos OB e controle (p=0,036), e OB+DP e OB comparado ao grupo DP (p=0.004 e p= 0.001, respectivamente). A obesidade parece afetar a espessura da parede da aorta em ratos Wistar. Entretanto, a presença de doença periodontal não afetou a espessura da parede da aorta sob as condições do presente estudo.


Asunto(s)
Animales , Masculino , Ratas , Periodontitis/complicaciones , Pérdida de Hueso Alveolar/etiología , Aterosclerosis , Ligadura/efectos adversos , Obesidad/complicaciones , Periodontitis/patología , Pérdida de Hueso Alveolar/patología , Ratas Wistar , Modelos Animales de Enfermedad
8.
Braz. oral res. (Online) ; 34: e015, 2020. tab, graf
Artículo en Inglés | LILACS | ID: biblio-1089381

RESUMEN

Abstract We sought to compare the characteristics and clinical significance of neutrophil extracellular traps in gingival samples from patients with periodontitis and those with gingivitis. The clinical indexes of gingival samples from patients with periodontitis and gingivitis were measured; the expression of TNF-alpha and IL-8 was measured by real-time fluorescent quantitative PCR; and the expression of TLR-8 and MMP-9 was measured by western blotting assays. Chemotaxis, phagocytosis and phagocytic activity of neutrophils were measured. Compared with the healthy group, the expression of TNF-α and IL-8 in the periodontitis group and the gingivitis group increased significantly (p < 0.05), and TNF-α in the gingivitis group was significantly lower than that in the healthy group (p < 0.05). The expression of IL-8 in the periodontitis group was significantly higher than that in the periodontitis group (p < 0.05). Furthermore, the expression of TLR-8 and MMP-9 in the periodontitis group was different from that in the gingivitis group and the healthy group, and the expression of TLR-8 and MMP-9 in the gingivitis group was significantly different from that in the healthy group (p < 0.05). In addition, the neutrophil mobility index in healthy people was 3.02 ± 0.53, that in the periodontitis group was 2.21 ± 0.13, and that in the gingivitis group was 2.31 ± 0.12. In conclusion, the chemotaxis of neutrophils in gingival samples of patients with periodontitis and gingivitis was decreased, the phagocytotic ability and activity of neutrophils were reduced, and the release of the extracellular trap-releasing inducible factors TNF-alpha and IL-8 also declined.


Asunto(s)
Humanos , Masculino , Femenino , Adulto Joven , Periodontitis/patología , Trampas Extracelulares , Gingivitis/patología , Neutrófilos/patología , Valores de Referencia , ARN/análisis , Estudios de Casos y Controles , Índice Periodontal , Western Blotting , Interleucina-8/análisis , Actinas/análisis , Factor de Necrosis Tumoral alfa/análisis , Metaloproteinasa 9 de la Matriz/análisis , Electroforesis en Gel de Agar , Receptor Toll-Like 8/análisis , Reacción en Cadena en Tiempo Real de la Polimerasa , Persona de Mediana Edad
9.
Braz. oral res. (Online) ; 34: e012, 2020. graf
Artículo en Inglés | LILACS | ID: biblio-1089395

RESUMEN

Abstract Lipoproteins are important bacterial immunostimulating molecules capable of inducing receptor activator of nuclear factor-κB (RANKL) and osteoclast formation in vitro and in vivo . Although these molecules are present in periodontopathogenic bacteria, their role in periodontitis is not known. In this study, we used Pam2CSK4 (PAM2), a synthetic molecule that mimics bacterial lipoprotein, to investigate the effects of lipoproteins on periodontitis in mice. C57BL/6 male mice were randomly divided into three experimental groups: 1) Negative control group: animals received vehicle injection; 2) Positive control group: animals received injection of Escherichia coli lipopolysaccharide (LPS); 3) PAM2 group: animals received PAM2 injection. All the injections were performed bilaterally every other day into the palatal mucosa between first and second molars. After twenty-four days, the animals were euthanized to assess alveolar bone volume (micro-CT), cellular and extracellular composition in the gingiva (stereometric analysis), and osteoclast numbers (TRAP staining). Treatment with either PAM2 or LPS induced gingival inflammation, as demonstrated by increased infiltration of inflammatory cells and enhanced angiogenesis, associated with a smaller number of fibroblasts and decreased extracellular matrix. Importantly, treatment not only with LPS but also with PAM2 resulted in a larger number of TRAP+ multinucleated osteoclasts and significant loss of alveolar bone. Collectively, our data demonstrate that PAM2 can induce gingival inflammation and bone loss in mice, broadening the avenues of investigation into the role of lipoproteins in the pathogenesis of periodontal disease.


Asunto(s)
Animales , Masculino , Periodontitis/etiología , Periodontitis/patología , Receptor Toll-Like 2/antagonistas & inhibidores , Lipopéptidos/farmacología , Osteoclastos/efectos de los fármacos , Osteoclastos/fisiología , Periodontitis/microbiología , Factores de Tiempo , Distribución Aleatoria , Pérdida de Hueso Alveolar/etiología , Pérdida de Hueso Alveolar/patología , Modelos Animales de Enfermedad , Microtomografía por Rayos X , Proceso Alveolar/efectos de los fármacos , Proceso Alveolar/patología , Fosfatasa Ácida Tartratorresistente , Encía/efectos de los fármacos , Encía/patología , Gingivitis/etiología , Gingivitis/patología , Ratones Endogámicos C57BL
10.
Artículo en Inglés | LILACS, BBO | ID: biblio-1135545

RESUMEN

Abstract Objective: To analyze whether the FokI polymorphism (rs228570) present in the vitamin D receptor gene in type 2 diabetics is related to chronic periodontitis's clinical status and evaluates the influence of chronic periodontitis on the perception of quality of life. Material and Methods: It is a clinical and laboratory study, composed of a sample of 59 individuals with previous diagnosis of type 2 Diabetes Mellitus and chronic periodontitis, of both sexes. On clinical examination, socio-epidemiological data and quality of life of patients with the Oral Health Impact Profile (OHIP-14) were recorded and a periogram was performed. Subsequently, saliva was collected spontaneously in sterile Falcon tubes (15 ml) and stored in the freezer at -20 °C. The purification of the genetic material was done with a PROMEGA kit (Wizard®), and the polymorphism studied was FokI (rs228570), found in the vitamin D receptor promoting region, with rs: 228570. After extraction of saliva DNA and purification, genotyping was performed by real-time PCR using specific allele probes (TaqMan® System). Results: The polymorphism of the vitamin D receptor gene was not positively associated with the severity and clinical characteristics of periodontitis, but suggested a relationship with the extent of the disease. Periodontitis also had no positive association with patients' perception of quality of life. Conclusion: The perception of quality of life of patients with chronic periodontitis and type 2 diabetes mellitus was compromised by the systemic condition, secondary to oral health, although some dimensions of OHIP-14 have been more frequently mentioned, such as psychological discomfort, physical pain and physical disability.


Asunto(s)
Humanos , Polimorfismo Genético , Calidad de Vida , Receptores de Calcitriol , Diabetes Mellitus Tipo 2/diagnóstico , Periodontitis Crónica/patología , Periodontitis/patología , Brasil , Distribución de Chi-Cuadrado , Salud Bucal , Estadísticas no Paramétricas , Reacción en Cadena en Tiempo Real de la Polimerasa/métodos
11.
Braz. oral res. (Online) ; 34: e038, 2020. tab, graf
Artículo en Inglés | LILACS, BBO | ID: biblio-1100932

RESUMEN

Abstract The possible role of B-cell growth and differentiation-related cytokines on the pathogenesis of diabetes-related periodontitis has not been addressed so far. The aim of this study was to evaluate the effects of diabetes mellitus (DM) on the gene expression of proliferation-inducing ligand (APRIL) and B-lymphocyte stimulator (BLyS), two major cytokines associated to survival, differentiation and maturation of B cells in biopsies from gingival tissue with periodontitis. Gingival biopsies were obtained from subjects with periodontitis (n = 17), with periodontitis and DM (n = 19) as well as from periodontally and systemically healthy controls (n = 10). Gene expressions for APRIL, BLyS, RANKL, OPG, TRAP and DC-STAMP were evaluated using qPCR. The expressions APRIL, BLyS, RANKL, OPG, TRAP and DC-STAMP were all higher in both periodontitis groups when compared to the control group (p < 0.05). Furthermore, the expressions of BLyS, TRAP and RANKL were significantly higher in the subjects with periodontitis and DM when compared to those with periodontitis alone (p < 0.05). The mRNA levels of BLyS correlated positively with RANKL in the subjects with periodontitis and DM (p < 0.05). BLyS is overexpressed in periodontitis tissues of subjects with type 2 DM, suggesting a possible role of this cytokine on the pathogenesis DM-related periodontitis.


Asunto(s)
Humanos , Masculino , Femenino , Adulto , Anciano , Periodontitis/inmunología , Periodontitis/patología , Diabetes Mellitus Tipo 2/complicaciones , Factor Activador de Células B/análisis , Osteogénesis/inmunología , Valores de Referencia , Biopsia , ARN Mensajero/análisis , Biomarcadores/análisis , Estudios de Casos y Controles , Expresión Génica , Citocinas/análisis , Citocinas/fisiología , Estadísticas no Paramétricas , Diabetes Mellitus Tipo 2/inmunología , Miembro 13 de la Superfamilia de Ligandos de Factores de Necrosis Tumoral/análisis , Reacción en Cadena en Tiempo Real de la Polimerasa , Encía/inmunología , Encía/patología , Persona de Mediana Edad
12.
Braz. oral res. (Online) ; 34: e012, 2020. graf
Artículo en Inglés | LILACS | ID: biblio-1055530

RESUMEN

Abstract Lipoproteins are important bacterial immunostimulating molecules capable of inducing receptor activator of nuclear factor-κB (RANKL) and osteoclast formation in vitro and in vivo . Although these molecules are present in periodontopathogenic bacteria, their role in periodontitis is not known. In this study, we used Pam2CSK4 (PAM2), a synthetic molecule that mimics bacterial lipoprotein, to investigate the effects of lipoproteins on periodontitis in mice. C57BL/6 male mice were randomly divided into three experimental groups: 1) Negative control group: animals received vehicle injection; 2) Positive control group: animals received injection of Escherichia coli lipopolysaccharide (LPS); 3) PAM2 group: animals received PAM2 injection. All the injections were performed bilaterally every other day into the palatal mucosa between first and second molars. After twenty-four days, the animals were euthanized to assess alveolar bone volume (micro-CT), cellular and extracellular composition in the gingiva (stereometric analysis), and osteoclast numbers (TRAP staining). Treatment with either PAM2 or LPS induced gingival inflammation, as demonstrated by increased infiltration of inflammatory cells and enhanced angiogenesis, associated with a smaller number of fibroblasts and decreased extracellular matrix. Importantly, treatment not only with LPS but also with PAM2 resulted in a larger number of TRAP+ multinucleated osteoclasts and significant loss of alveolar bone. Collectively, our data demonstrate that PAM2 can induce gingival inflammation and bone loss in mice, broadening the avenues of investigation into the role of lipoproteins in the pathogenesis of periodontal disease.


Asunto(s)
Animales , Masculino , Periodontitis/etiología , Periodontitis/patología , Receptor Toll-Like 2/antagonistas & inhibidores , Lipopéptidos/farmacología , Osteoclastos/efectos de los fármacos , Periodontitis/microbiología , Factores de Tiempo , Distribución Aleatoria , Pérdida de Hueso Alveolar/etiología , Pérdida de Hueso Alveolar/patología , Modelos Animales de Enfermedad , Microtomografía por Rayos X , Proceso Alveolar/efectos de los fármacos , Proceso Alveolar/patología , Fosfatasa Ácida Tartratorresistente , Encía/efectos de los fármacos , Encía/patología , Gingivitis/etiología , Gingivitis/patología , Ratones Endogámicos C57BL
13.
J. appl. oral sci ; 28: e20190140, 2020. tab, graf
Artículo en Inglés | LILACS, BBO | ID: biblio-1056590

RESUMEN

Abstract Objective: The goal of the present study was to determine the effect of systemic and topical ozone application on alveolar bone loss (ABL) by evaluating the effect of Hypoxia-inducible factor −1 alpha (HIF-1-α) and receptor activator of NF-kB ligand (RANKL)-positive cells on histopathological and immunohistochemical changes in a rat periodontitis model. Methodology: Thirty male Wistar rats were divided into three groups: 1) Group C (control group); 2) Group SO (systemic ozone group) and 3) Group TO (topical ozone group). Experimental periodontitis was induced with a 3/0 silk suture placed at the mandibular left first molars of rats, and the suture was removed 14 days later. Ozone gas was injected intraperitoneally (0.7 mg/kg) in SO group. Topical ozone application protocol was performed using an ozone generator at 80% concentration (4th grade) 90- degree probe for the duration of 30 s. Both ozone applications were carried out for two weeks at intervals of two days. Histomorphometric and immunohistochemical analysis were performed. Results: ABL was significantly lower in Group SO compared to Group C (p: 0.0052). HIF-1α- positive cells were significantly lower in Group TO than in Group C (p: 0.0043). RANKL-positive cells were significantly lower in Group SO and in Group TO compared to the control group (p: 0.0033, p: 0.0075, respectively). Conclusion: Both ozone applications decreased RANKL-positive cell counts, TO application decreased HIF-1-α positive cells counts, and SO application was found to be more effective in reducing ABL compared to control group.


Asunto(s)
Animales , Masculino , Ozono/administración & dosificación , Periodontitis/patología , Periodontitis/tratamiento farmacológico , Pérdida de Hueso Alveolar/patología , Pérdida de Hueso Alveolar/tratamiento farmacológico , Subunidad alfa del Factor 1 Inducible por Hipoxia/análisis , Inmunohistoquímica , Recuento de Células , Reproducibilidad de los Resultados , Administración Tópica , Resultado del Tratamiento , Ratas Wistar
14.
J. appl. oral sci ; 27: e20180316, 2019. tab
Artículo en Inglés | LILACS, BBO | ID: biblio-984569

RESUMEN

Abstract Objective The aim of this study was to evaluate the levels of salivary biomarkers IL-1β, IL-10, RANK, OPG, MMP-2, TG-β and TNF-α in individuals with diagnosis of peri-implant mucositis in the absence or presence of periodontal and peri-implant maintenance therapy (TMPP) over 5 years. Material and Methods Eighty individuals diagnosed with peri-implant mucositis were divided into two groups: one group that underwent periodontal and peri-implant regularly maintenance therapy, called GTP (n=39), and a second group that received no regular maintenance GNTP (n=41). Each participant underwent a complete periodontal and peri-implant clinical examination. Collection of saliva samples and radiographic examination to evaluate peri-implant bone levels were conducted at two times: initial examination (T1) and after 5 years (T2). The salivary samples were evaluated through ELISA for the following markers: IL-1β, IL-10, RANK, OPG, MMP-2, TGF and TNF-α. Results A higher incidence of peri-implantitis was observed in the GNTP group (43.9%) than in the GTP group (18%) (p=0.000). All individuals (n=12) who presented peri-implant mucositis and had resolution at T2 were in the GTP group. After 5 years, there was an increase in the incidence of periodontitis in the GNTP group compared to the GTP group (p=0.001). The results of the study revealed an increase in the salivary concentration of TNF-α in the GNTP group compared to the GTP group. The other salivary biomarkers that were evaluated did not show statistically significant differences between the two groups. Conclusions The salivary concentration of TNF-α was increased in individuals with worse periodontal and peri-implant clinical condition and in those with a higher incidence of peri-implantitis, especially in the GNTP group. Longitudinal studies in larger populations are needed to confirm these findings and elucidate the role of this biomarker in peri-implant disease.


Asunto(s)
Humanos , Periodontitis/patología , Saliva/química , Estomatitis/patología , Implantes Dentales/efectos adversos , Citocinas/análisis , Receptor Activador del Factor Nuclear kappa-B/análisis , Osteoprotegerina/análisis , Periodontitis/diagnóstico , Valores de Referencia , Estomatitis/diagnóstico , Ensayo de Inmunoadsorción Enzimática , Biomarcadores/análisis , Estudios de Casos y Controles , Factores de Riesgo , Estudios de Seguimiento , Estadísticas no Paramétricas , Progresión de la Enfermedad
15.
J. appl. oral sci ; 27: e20180211, 2019. tab, graf
Artículo en Inglés | LILACS, BBO | ID: biblio-984568

RESUMEN

Abstract Objective The aim of this study was to evaluate the effects of gliclazide on oxidative stress, inflammation, and bone loss in an experimental periodontal disease model. Material and Methods Male albino Wistar rats were divided into no ligature, ligature, and ligature with 1, 5, and 10 mg/kg gliclazide groups. Maxillae were fixed and scanned using micro-computed tomography to quantify linear and bone volume/tissue volume (BV/TV) and volumetric bone loss. Histopathological, immunohistochemical and immunofluorescence analyses were conducted to examine matrix metalloproteinase-2 (MMP-2), cyclooxygenase 2 (COX-2), cathepsin K, members of the receptor activator of the nuclear factor kappa-Β ligand (RANKL), receptor activator of nuclear factor kappa-Β (RANK), osteoprotegerin (OPG) pathway, macrophage migration inhibitory factor (MIF), superoxide dismutase-1 (SOD-1), glutathione peroxidase-1 (GPx-1), NFKB p 50 (Cytoplasm), NFKB p50 NLS (nuclear localization signal), PI3 kinase and AKT staining. Myeloperoxidase activity, malondialdehyde and glutathione levels, while interleukin-1 beta (IL-1β) and tumor necrosis factor-alpha (TNF-α) levels were evaluated by spectroscopic ultraviolet-visible analysis. A quantitative reverse transcription polymerase chain reaction was used to quantify the gene expression of the nuclear factor kappa B p50 subunit (NF-κB p50), phosphoinositide 3-kinase (PI3k), protein kinase B (AKT), and F4/80. Results Micro-computed tomography showed that the 1 mg/kg gliclazide treatment reduced linear bone loss compared to the ligature, 5 mg/kg gliclazide, and 10 mg/kg gliclazide treatments. All concentrations of gliclazide increased bone volume/tissue volume (BV/TV) compared to the ligature group. Treatment with 1 mg/kg gliclazide reduced myeloperoxidase activity, malondialdehyde, IL-1β, and TNF-α levels (p≤0.05), and resulted in weak staining for COX-2, cathepsin k, MMP-2, RANK, RANKL, SOD-1, GPx-1,MIF and PI3k. In addition, down-regulation of NF-κB p50, PI3k, AKT, and F4/80 were observed, and OPG staining was strong after the 1 mg/kg gliclazide treatment. Conclusions This treatment decreased neutrophil and macrophage migration, decreased the inflammatory response, and decreased bone loss in rats with ligature-induced periodontitis.


Asunto(s)
Animales , Masculino , Periodontitis/tratamiento farmacológico , Pérdida de Hueso Alveolar/tratamiento farmacológico , Estrés Oxidativo/efectos de los fármacos , Gliclazida/farmacología , Antioxidantes/farmacología , Periodontitis/patología , Inmunohistoquímica , Distribución Aleatoria , Reproducibilidad de los Resultados , Pérdida de Hueso Alveolar/patología , Técnica del Anticuerpo Fluorescente , Factores Inhibidores de la Migración de Macrófagos/efectos adversos , Factor de Necrosis Tumoral alfa/análisis , Ratas Wistar , Peroxidasa/análisis , Reacción en Cadena de la Polimerasa de Transcriptasa Inversa , Metaloproteinasa 2 de la Matriz/análisis , Interleucina-1beta/análisis , Ligando RANK/análisis , Receptor Activador del Factor Nuclear kappa-B/análisis , Microtomografía por Rayos X , Catepsina K/análisis , Encía/patología , Encía/química , Gliclazida/uso terapéutico , Glutatión/análisis , Malondialdehído/análisis , Neutrófilos/efectos de los fármacos , Antioxidantes/uso terapéutico
16.
Braz. oral res. (Online) ; 33: e001, 2019. graf
Artículo en Inglés | LILACS | ID: biblio-989482

RESUMEN

Abstract Colchicine is widely used in the treatment of several inflammatory diseases due to its anti-inflammatory effect, but effects on bone metabolism are unclear. The aim of this study was to evaluate the effects of systemically-administered colchicine on healthy periodontium and experimentally-induced periodontitis. In total, 42 male Wistar rats were included in this study. A non-ligated group constituting the negative control group (Control, C, n = 6) and a ligature-only group forming the positive control group (LO, n = 12) were created separately. Twelve rats were treated with 0.4 mg/kg colchicine and another 12 with 1 mg/kg colchicine. In the colchicine-administered groups, right mandibles constituted the ligated groups (1 mgC-L or 0.4 mgC-L) and left mandibles formed the corresponding non-ligated controls (1mgC or 0.4mgC). Silk ligatures were placed at the gingival margin of the lower first molars. The animals were euthanized at different time-points of healing (11 or 30 days). Alveolar bone loss was clinically measured and TRAP+ osteoclasts, osteoblastic activity, and MMP-1 expression were examined histologically. There was no increase in alveolar bone loss with either colchicine dose in healthy periodontium (p > 0.05) and the highest level of alveolar bone loss, TRAP+ osteoclast number, and MMP-1 expression were measured in the LO group (p < 0.05). The 0.4 mgC-L group showed less alveolar bone loss at 11 days (p < 0.05), but greater loss at 30 days. The 1 mgC-L group showed higher osteoblast number than the other ligated groups (p < 0.05) at both time-points. In summary, colchicine did not increase alveolar bone loss in healthy periodontium and also may tend to reduce periodontitis progression. However, further extensive study is necessary to understand the mechanism of colchicine action on alveolar bone loss in periodontitis.


Asunto(s)
Humanos , Animales , Masculino , Periodontitis/tratamiento farmacológico , Colchicina/farmacología , Pérdida de Hueso Alveolar/tratamiento farmacológico , Antiinflamatorios/farmacología , Osteoblastos/efectos de los fármacos , Osteoclastos/efectos de los fármacos , Periodontitis/etiología , Periodontitis/patología , Factores de Tiempo , Inmunohistoquímica , Colchicina/uso terapéutico , Reproducibilidad de los Resultados , Pérdida de Hueso Alveolar/patología , Resultado del Tratamiento , Ratas Wistar , Metaloproteinasa 1 de la Matriz/análisis , Moduladores de Tubulina/farmacología , Fosfatasa Ácida Tartratorresistente/análisis , Ligadura , Antiinflamatorios/uso terapéutico
17.
J. appl. oral sci ; 27: e20180713, 2019. tab, graf
Artículo en Inglés | LILACS, BBO | ID: biblio-1040234

RESUMEN

Abstract Vitamin D has been known to have important regulatory functions in inflammation and immune response and shows inhibitory effects on experimental periodontitis in animal models. However, the potential mechanism has yet to be clarified. Recent studies have highlighted Aryl hydrocarbon receptor (AhR) and its downstream signaling as a crucial regulator of immune homeostasis and inflammatory regulation. Objective: This study aimed to clarify the effect of 1,25-dihydroxyvitamin D3 (VD3) on experimental periodontitis and AhR/nuclear factor-κB (NF-κB)/NLR pyrin domain-containing 3 (NLRP3) inflammasome pathway in the gingival epithelium in a murine model. Methodology: We induced periodontitis in male C57BL/6 wild-type mice by oral inoculation of Porphyromonas gingivalis (P. gingivalis), and subsequently gave intraperitoneal VD3 injection to the mice every other day for 8 weeks. Afterwards, we examined the alveolar bone using scanning electron microscopy (SEM) and detected the gingival epithelial protein using western blot analysis and immunohistochemical staining. Results: SEM images demonstrated that alveolar bone loss was reduced in the periodontitis mouse model after VD3 supplementation. Western blot analyses and immunohistochemical staining of the gingival epithelium showed that the expression of vitamin D receptor, AhR and its downstream cytochrome P450 1A1 were enhanced upon VD3 application. Additionally, VD3 decreased NF-κB p65 phosphorylation, and NLRP3, apoptosis-associated speck-like protein, caspase-1, interleukin-1β (IL-1β) and IL-6 protein expression. Conclusions: These results implicate the alleviation of periodontitis and the alteration of AhR/NF-κB/NLRP3 inflammasome pathway by VD3 in the mouse model. The attenuation of this periodontal disease may correlate with the regulation of AhR/NF-κB/NLRP3 inflammasome pathway by VD3.


Asunto(s)
Animales , Masculino , Periodontitis/metabolismo , Periodontitis/tratamiento farmacológico , Calcitriol/farmacología , FN-kappa B/efectos de los fármacos , Conservadores de la Densidad Ósea/farmacología , Proteína con Dominio Pirina 3 de la Familia NLR/efectos de los fármacos , Periodontitis/patología , Valores de Referencia , Calcitriol/análisis , Inmunohistoquímica , Western Blotting , Reproducibilidad de los Resultados , Pérdida de Hueso Alveolar , FN-kappa B/análisis , Interleucina-6/análisis , Resultado del Tratamiento , Receptores de Hidrocarburo de Aril/análisis , Receptores de Hidrocarburo de Aril/efectos de los fármacos , Porphyromonas gingivalis , Caspasa 1/análisis , Conservadores de la Densidad Ósea/análisis , Interleucina-1beta/análisis , Proteína con Dominio Pirina 3 de la Familia NLR/análisis , Encía/efectos de los fármacos , Encía/metabolismo , Encía/patología , Ratones Endogámicos C57BL
18.
J. appl. oral sci ; 27: e20180602, 2019. graf
Artículo en Inglés | LILACS, BBO | ID: biblio-1019974

RESUMEN

Abstract Objective: This study aimed to evaluate the effect of avocado/soybean unsaponifiables (ASU) on periodontal repair in rats with induced periodontitis and arthritis. Methodology: Forty-five rats were submitted to periodontitis induction by insertion of ligatures into the upper second molars, maintained for 15 days. These animals were randomly allocated to 3 groups according to the presence of induced arthritis (ART) and the application of the ASU: Control (CTR) group-healthy animals, where saline solution was administered; ART-animals with induced arthritis, where saline solution was administered; ART/ASU-animals with induced arthritis, where ASU (0.6 mg/ kg) was administered. The drugs were administered daily by gavage and the animals were euthanized after 7, 15 and 30 days of the ligature removal. Bone resorption, inflammatory infiltrate composition and marker proteins expression of the differentiation and formation of osteoclasts (RANKL and TRAP) were assessed. Results: The ART/ASU group presented higher bone volume than the ART group at 7 and 30 days after the ligature removal. Furthermore, the ART group presented higher quantity of inflammatory cells and expression of TRAP and RANKL than the other groups. Conclusion: ASU administration improves the repair of periodontal tissues in an experimental periodontitis model in rats with induced arthritis.


Asunto(s)
Animales , Masculino , Ratas , Periodontitis/tratamiento farmacológico , Artritis/tratamiento farmacológico , Glycine max/química , Extractos Vegetales/farmacología , Persea/química , Periodontitis/patología , Artritis/patología , Factores de Tiempo , Inmunohistoquímica , Distribución Aleatoria , Reproducibilidad de los Resultados , Resultado del Tratamiento , Ligando RANK/análisis , Microtomografía por Rayos X , Fosfatasa Ácida Tartratorresistente/análisis
19.
Braz. oral res. (Online) ; 33: e025, 2019. graf
Artículo en Inglés | LILACS | ID: biblio-1001603

RESUMEN

Abstract: Recently, it has been suggested that the anti-inflammatory hormone ghrelin (GHRL) and its receptor GHS-R may play a pivotal role in periodontal health and diseases. However, their exact regulation and effects in periodontitis are not known. The aim of this in-vitro study was to investigate the effect of microbial and inflammatory insults on the GHS-R1a expression in human osteoblast-like cells. MG-63 cells were exposed to interleukin (IL)-1β and Fusobacterium nucleatum in the presence and absence of GHRL for up to 2 d. Subsequently, gene expressions of GHS-R1a, inflammatory mediators and matrix metalloproteinase were analyzed by real-time PCR. GHS-R protein synthesis and NF-κB p65 nuclear translocation were assessed by immunocytochemistry and immunofluorescence microscopy, respectively. IL-1β and F. nucleatum caused a significant upregulation of GHS-R1a expression and an increase in GHS-R1a protein. Pre-incubation with a MEK1/2 inhibitor diminished the IL-1β-induced GHS-R1a upregulation. IL-1β and F. nucleatum also enhanced the expressions of cyclooxygenase 2, CC-chemokine ligand 2, IL-6, IL-8, and matrix metalloproteinase 1, but these stimulatory effects were counteracted by GHRL. By contrast, the stimulatory actions of IL-1β and F. nucleatum on the GHS-R1a expression were further enhanced by GHRL. Our study provides original evidence that IL-1β and F. nucleatum regulate the GHS-R/GHRL system in osteoblast-like cells. Furthermore, we demonstrate for the first time that the proinflammatory and proteolytic actions of IL-1β and F. nucleatum on osteoblast-like cells are inhibited by GHRL. Our study suggests that microbial and inflammatory insults upregulate GHS-R1a, which may represent a protective negative feedback mechanism in human bone.


Asunto(s)
Humanos , Osteoblastos/química , Fusobacterium nucleatum/fisiología , Interleucina-1beta/farmacología , Receptores de Ghrelina/análisis , Osteoblastos/efectos de los fármacos , Osteoblastos/microbiología , Periodontitis/microbiología , Periodontitis/patología , Inmunohistoquímica , Regulación hacia Arriba/fisiología , Células Cultivadas , Análisis de Varianza , Estadísticas no Paramétricas , Receptores de Ghrelina/fisiología , Reacción en Cadena en Tiempo Real de la Polimerasa , Microscopía Fluorescente
20.
Braz. oral res. (Online) ; 33: e032, 2019. tab, graf
Artículo en Inglés | LILACS | ID: biblio-1001608

RESUMEN

Abstract: This study aimed to investigate the effects of astragaloside IV (AsIV) on inflammation and immunity in rats with experimental periodontitis. Periodontitis was established in 48 Wistar rats, which were then randomly divided into model and 10, 20 and 40 mg/kg AsIV groups, with 12 rats in each group. The latter 3 groups were treated with AsIV at doses of 10, 20 and 40 mg/kg, respectively. The control group (12 rats, without periodontitis) and model group were given the same amount of 5% sodium carboxymethyl cellulose. The treatment was performed once per day for 8 weeks. Before and after treatment, the tooth mobility scores of the rats were determined. After treatment, the salivary occult blood index (SOBI), plaque index (PLI), peripheral blood T lymphocyte subsets, and serum inflammatory factor and immunoglobulin levels were determined. The results showed that, after treatment, compared with that in model group, in 40 mg/kg AsIV group, the general state of rats was improved, while the tooth mobility score, SOBI and PLI were significantly decreased (p < 0.05); the peripheral blood CD4+ T cell percentage and CD4+/CD8+ ratio were significantly increased (p < 0.05), while the CD8+ T cell percentage was significantly decreased (p < 0.05); the serum tumor necrosis factor-α, interleukin-1β and interleukin-2 levels were significantly decreased (p < 0.05); the serum immunoglobulin A and immunoglobulin G levels were significantly decreased (p < 0.05). In conclusion, AsIV can alleviate inflammation and enhance immunity in rats with experimental periodontitis.


Asunto(s)
Animales , Masculino , Femenino , Periodontitis/tratamiento farmacológico , Saponinas/farmacología , Triterpenos/farmacología , Sistema Inmunológico/efectos de los fármacos , Periodontitis/inmunología , Periodontitis/patología , Valores de Referencia , Movilidad Dentaria , Inmunoglobulinas/sangre , Distribución Aleatoria , Reproducibilidad de los Resultados , Subgrupos de Linfocitos T , Interleucina-2/sangre , Factor de Necrosis Tumoral alfa/sangre , Resultado del Tratamiento , Ratas Wistar , Interleucina-1beta/sangre
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