RESUMEN
Larvae, nymphs, and adult stages of 3 species of ixodid ticks were collected by tick drag methods in Seoul during June-October 2013, and their infection status with severe fever with thrombocytopenia syndrome (SFTS) virus was examined using RT-PCR. During the period, 732 Haemaphysalis longicornis, 62 Haemaphysalis flava, and 2 Ixodes nipponensis specimens were collected. Among the specimens of H. longicornis, the number of female adults, male adults, nymphs, and larvae were 53, 11, 240, and 446, respectively. Ticks were grouped into 63 pools according to the collection site, species, and developmental stage, and assayed for SFTS virus. None of the pools of ticks were found to be positive for SFTS virus gene.
Asunto(s)
Animales , Femenino , Humanos , Masculino , Fiebre/virología , Ixodidae/clasificación , Larva/patogenicidad , Fiebre por Flebótomos/virología , Phlebovirus/aislamiento & purificación , República de Corea , Trombocitopenia/virología , Infestaciones por Garrapatas/virologíaRESUMEN
No abstract available.
Asunto(s)
Animales , Humanos , Mordeduras y Picaduras de Insectos/virología , Fiebre por Flebótomos/etiología , Phlebovirus/aislamiento & purificación , Garrapatas/virologíaRESUMEN
Specificity and sensitivity are very important when investigating the blood meals of an insect vector, particularly those having different hosts. Several techniques are used for the identification of the blood meals. Counter immunoelectrophoresis is the technique of choice. The modification was to secure saving of time and least amount of materials. In this paper, a modified technique of the counter immunoelectrophoresis [CIEP] was used for the identification of the blood meal of wild Phlebotomus papatasi collected from different sites in Qalyoubia Governorate. Eight antisera were used. These were human, cat, dog, rat, sheep, goat, bovine, and avian. The results showed that 99.52% of 832 female P. papatasi contained human blood, 0.12% [one female] contained only avian blood, and 0.36% or three females each contained mixed blood of human together with dog blood [one], rat blood [one] or avian blood [one]. P. papatasi is an anthropophilic insect, but in rare cases, it is zoophilic