Antibiotic susceptibility profile of bacilli isolated from the skin of healthy humans

Abstract In the present work, twelve bacilli were isolated from four different regions of human skin from Bela population of Nagpur district, India. The isolated bacilli were identified by their morphological, cultural and biochemical characteristics. Seven isolates were Gram negative rods, out of which five were belong to genus Pseudomonas. Three among the five Gram positive isolates were identified as Dermabactor and the remaining two Bacillus. Their antimicrobial susceptibility profile was determined by Kirby-Bauer disc diffusion method. The isolates showed resistance to several currently used broad-spectrum antibiotics. The Dermabactor genus was resistant to vancomycin, although it was earlier reported to be susceptible. Imipenem was found to be the most effective antibiotic for Pseudomonas while nalidixic acid, ampicillin and tetracycline were ineffective. Isolates of Bacillus displayed resistance to the extended spectrum antibiotics cephalosporin and ceftazidime. Imipenem, carbenicillin and ticarcillin were found to be the most effective antibiotics as all the investigated isolates were susceptible to them. Antibiotic resistance may be due to the overuse or misuse of antibiotics during the treatment, or following constant exposure to antibiotic-containing cosmetic formulations.

relationship of skin type with minimal erythema dose [MED]

Phototherapy is an important modality in dermatology and the number of skin diseases which can be controlled with it is increasing. In order to start treatment, the first dose of phototherapy is determined by measurement of minimal erythema dose [MED] in each patient individually or according to patient's skin type. To determine the relationship of skin type with MED in patients under UV phototherapy referred to Razi Hospital in Tehran .In patients referred to Razi Hospital in 1381[2002-2003] for UVB phototherapy, skin type was determined according to Fitzpatrick's classification and MED was measured using an UV-meter. Age, sex and the disease of the patients were also recorded. The data were analysed using one way ANOVA and Welch tests. Eighty patients including 39 males and 41 females were studied. The mean age of the patients was 32 years [SD=15.8]. Their diseases include psoriasis, vitiligo, parapsoriasis, pityriasis lichenoides chronica, perforating disorder and patch stage mycosis fungoides. The mean of MED in 17 patients with skin type II was 111.8 +/- 39.2, in 42 patients with skin type III was 105.9 +/- 43.1, and in 21 patients with skin type IV was 114.3 +/- 39.2 mJ/cm [2], with no significant statistical difference. The first dose of phototherapy could not be determined based on skin type and measurement of MED is necessary in each patient