Colestasis gravidica: a propósito de un caso / Cholestasis gravidarum: a purpose of a case

La Colestasis Gravídica en un síndrome que habitualmente se produce en el tercer trimestre del embarazo y se resuelve en el embarazo. Se caracteriza clínicamente por la presencia de prurito, ictericia o ambos sin otra disfunción hepática importante. En el siguiente trabajo se reporta caso de paciente femenino de 24 años III gestas II para FUR: 12/8/06 quien refiere de 6 días de evolución orinas coluricas, prurito generalizado predominio de manos y tórax, exámenes para clínicos con aumento de un transaminasas, fosfatasas alcalinas, se diagnóstica colestasis gravídica, patología de baja incidencia de 1/300 y 1/2000 embarazos, se considera importante realizar una revisión académica del mismo.

Effects of IL 6 on steroidogenesis of human granulosa cells in vitro

Recent studies suggest that in addition to gonadotropins, immunological factors, such as cytokines play an important role in production of steroid hormones. The purpose of this study was to examine effects of IL-6 on basal and FSH stimulated secretions of estradiol and progesterone in the presence of androstendione by human granulosa cells [GC] in vitro. Graunlosa cells were harvested at the time of follicular aspiration after ovarian hyperstimulation according to standard protocols with hMG from patients undergoing IVFET. The cells [2 X 10[4] viable cells per well] were cultured with HAM and # 101; s F-10 without any supplements [control] or increasing concentrations of recombinant human [rh] IL-6, [8,16,32,64,128 pg/ml] added in the absence or presence of FSH [96 IU/ml]. Media were collected after 24,48,72 and 96 hours at a 24h interval and estradiol and progesterone levels were measured by an enzyme immunoassay [EIA] with automated system. Results of this study showed that leuteinized GC in the absence of FSH and the presence of androgen was able to produce estradiol and progesterone in vitro. This production was significantly increased in the presence of FSH. Basal and FSH stimulated productions of estradiol were significantly [P < 0.05] inhibited by increasing amounts of IL-6. Although this inhibitory effect on basal production of progesterone was not significant. IL-6 in a dose-dependent manner significantly [P < 0.05] inhibited FSH stimulated production of progesterone by GC. These results suggest that IL-6 may play an important role in the production of estradiol and progesterone and any disorders in level of IL-6 may cause estradiol and progesterone release disturbances

Production and characterisation of a monoclonal antibody to progesterone and its effect on fertility.

A monoclonal antibody reacting with progesterone has been raised by fusion of mouse myeloma cells (SP20) and splenocytes of BALB/c mice hyperimmunized with 4-pregnane 3,20 dione conjugated to bovine serum albumin. Association constant of this antibody for binding with progesterone was 0.22 x 10(9) l/mole. The antibody was highly specific for progesterone. A single ip injection of this antibody brought about an antifertility effect which is influenced by genotype. Antibody treatment brought about a significant decrease in the fetal weight and a slight decrease in the plasma progesterone levels. The antifertility effect could be reversed only up to day 3 by exogenous administration of progesterone.