Propofol attenuates cytokine-mediated upregulation of expression of inducible nitric oxide synthase and apoptosis during regeneration post-partial hepatectomy

Abstract Purpose: To determine the effects of propofol and ketamine anesthesia on liver regeneration in rats after partial hepatectomy (PHT). Methods: Male Wistar albino rats were assigned randomly to four groups of 10. Anesthesia was induced and maintained with propofol in groups 1 and 2, and with ketamine in groups 3 and 4. PHT was undertaken in groups 1 and 3. Rats in groups 2 and 4 (control groups) underwent an identical surgical procedure, but without PHT. At postoperative day-5, rats were killed. Regenerated liver was removed, weighed, and evaluated (by immunohistochemical means) for expression of inducible nitric oxide synthase (iNOS), endothelial NOS (eNOS), apoptosis protease-activating factor (APAF)-1, and proliferating cell nuclear antigen (PCNA). Also, blood samples were collected for measurement of levels of tumor necrosis factor (TNF)-α and interleukin (IL)-6. Results: Between groups 2 and 4, there were no differences in tissue levels of iNOS, eNOS, and APAF-1 or plasma levels of TNF-α and IL-6. eNOS expression was similar in group 1 and group 3. Expression of iNOS and APAF-1 was mild-to-moderate in group 1, but significantly higher in group 3. Groups 1 and 3 showed an increase in PCNA expression, but expression in both groups was comparable. Plasma levels of TNF-α and IL-6 increased to a lesser degree in group 1 than in group 3. Conclusion: Propofol, as an anesthetic agent, may attenuate cytokine-mediated upregulation of iNOS expression and apoptosis in an animal model of liver regeneration after partial hepatectomy.

Propofol pretreatment attenuates lipopolysaccharide-induced acute lung injury in rats by activating the phosphoinositide-3-kinase/Akt pathway

The aim of this study was to investigate the effect of propofol pretreatment on lipopolysaccharide (LPS)-induced acute lung injury (ALI) and the role of the phosphoinositide-3-kinase/protein kinase B (PI3K/Akt) pathway in this procedure. Survival was determined 48 h after LPS injection. At 1 h after LPS challenge, the lung wet- to dry-weight ratio was examined, and concentrations of protein, tumor necrosis factor-α (TNF-α), and interleukin-6 (IL-6) in bronchoalveolar lavage fluid (BALF) were determined using the bicinchoninic acid method or ELISA. Lung injury was assayed via lung histological examination. PI3K and p-Akt expression levels in the lung tissue were determined by Western blotting. Propofol pretreatment prolonged survival, decreased the concentrations of protein, TNF-α, and IL-6 in BALF, attenuated ALI, and increased PI3K and p-Akt expression in the lung tissue of LPS-challenged rats, whereas treatment with wortmannin, a PI3K/Akt pathway specific inhibitor, blunted this effect. Our study indicates that propofol pretreatment attenuated LPS-induced ALI, partly by activation of the PI3K/Akt pathway.

Propofol: un nuevo anestésico intravenoso / Propofol: of a new intravenous anesthetics

Prpopofol (DIprivan) as induction and maintenance anaesthetic was in 35 patients A.S.A. I and II. Patients scheduled for different surgery. Pre inclution was with benzodiazepin 10 mg and atropi 0,001 followed 5 - 10 minutes later by 2-2,5 mg/kg op Propofol (Diprivan) under proxigenation. Aditonal fixed dosis of Propofol (Diprivan) were given apnea after induction was present in most of the patients, manual ventilation throgh a face mask. Cardiovascular parameters were more o less stable. A psychometric test consisting in identifying some words and to answer earmar king. No side efects were detected and anaesthesia was weel acepted by every patients entering this study with respect to efficacy and safety.

Cambios en los niveles plasmáticos de glucosa en pacientes sometidos a cirugía otorrinolaringológica electiva con anestesia general intravenosa total con propofol vs anestesia general inhalatoria con halotano / Changes in plasma levels of glucose in patients scheduled for ONT procedures during propofol anesthesia versus halothame anesthesia

El presente trabajo describe los efectos de la anestesia total intravenosa con propofol versus anestesia general con halotano, en cirugia otorrinolaringológica. Se formaron dos grupos de pacientes con el objeto de evaluar los niveles plasmáticos de glucosa con estas dos técnicas anestésicas. Se encontró un ligero incremento estadísticamente significativo, en los niveles de glucosa en el grupo de pacientes que recibió anestesia total intravenosa con propofol. Tal incremento no mostró repercución clínica. Se discute la importancia de estos hallazgos en la practica diaria de la anestesia