Cloning, expression and purification of neural specific HuD cDNA / 中国医学科学院学报

<p><b>OBJECTIVE</b>To prokaryoticly express and purify HuD protein and its RNA recognition motifs.</p><p><b>METHODS</b>HuD protein was prokaryoticly expressed and purified by molecular cloning technology. Its biologic activity was testified by Western Blot.</p><p><b>RESULTS</b>Purified HuD protein and its RNA recognized motifs were observed.</p><p><b>CONCLUSIONS</b>The result might aid for basic research and clinical application.</p>