Isolamento e identificação de enterococcus sp em infecções endodônticas primárias / Isolation and identification of enterococcus sp in primary endodontic infections

Este estudo objetivou o isolamento e caracterização bioquímica de Enterococcus sp de canais radiculares portadores de necrose pulpar de 70 pacientes. Foram isoladas 35 amostras de Enterococcus sp em 50% dos pacientes (n = 35) com a seguinte prevalência: 33 amostras Enterococcus faecalis (94,28%), 1 amostra Enterococcus faecium (2,85%) e 1 amostra Enterococcus durans (2,85%). Dos dentes com cultura positiva, 20 apresentaram sintomatologia dolorosa. Odor fétido e secreção purulenta foram observados em três dentes (8,57%). Dos 35 pacientes com cultura positiva, quatro apresentaram edema no momento da coleta e apenas um paciente apresentou fístula. A espécie E. faecalis foi majoritária, estando relacionada aos sinais e sintomas.

Analysis of Enterococcus faecalis in samples from Turkish patients with primary endodontic infections and failed endodontic treatment by real-time PCR SYBR green method

OBJECTIVE: The aims of this study were to investigate the presence of Enterococcus faecalis in primary endodontic infections and failed endodontic treatments using real-time PCR and to determine the statistical importance of the presence of E. faecalis in a Turkish population with endodontic infections. MATERIAL AND METHODS: E. faecalis was investigated from 79 microbial samples collected from patients who were treated at the Endodontic Clinic of the Dental School of Atatürk University (Erzurum, Turkey). Microbial samples were taken from 43 patients (Group 1) with failed endodontic treatments and 36 patients (Group 2) with chronic apical periodontitis (primary endodontic infections). DNA was extracted from the samples by using a QIAamp® DNA mini-kit and analyzed with real-time PCR SYBR Green. RESULTS: E. faecalis was detected in 41 out of 79 patients, suggesting that it exists in not less than 61 percent of all endodontic infections when the proportion test (z= -1.645,