Your browser doesn't support javascript.
loading
Mostrar: 20 | 50 | 100
Resultados 1 - 2 de 2
Filtrar
1.
Artículo en Inglés | IMSEAR | ID: sea-163229

RESUMEN

1KE8 is known as a potential target for anti-cancer medication. Indoles are biologically active nitrogen heterocyclics known for broad spectrum activities. Modification of Indole ring system with selected structural descriptors has offered a high degree of stereo specificity and diversity in activity to the moiety.


Asunto(s)
Antineoplásicos/farmacocinética , Sitios de Unión , Simulación por Computador , Quinasa 8 Dependiente de Ciclina/metabolismo , Compuestos Heterocíclicos con 2 Anillos/farmacocinética , Indoles/clasificación , Indoles/farmacocinética , Simulación del Acoplamiento Molecular , Simulación de Dinámica Molecular
2.
Protein & Cell ; (12): 911-920, 2013.
Artículo en Inglés | WPRIM | ID: wpr-757559

RESUMEN

Mediator is a highly conserved large protein complex (25 proteins, >1000 kDa) and preeminently responsible for eukaryotic transcription, which contains a dissociable 'Cdk8 module'. Although increasing evidence demonstrates that Cdk8 module plays both positive and negative roles in transcription regulation, the detailed structure, and subunit organization, molecular mechanism how it regulates transcription remain elusive. Here we used single-particle electron microscopy to characterize the structure and subunit organization of the Cdk8 module and illuminated the substantial mobility of the Med13 subunit results in the structural flexibility. The Cdk8 module interaction with core Mediator is concurrent with active transcription in vivo. An interaction with the Cdk8 module induces core Mediator into very extended conformation in vitro, which is presumed to be an active functional state of Mediator. Taken together, our results illuminated the detailed architecture of Cdk8 module, and suggested the Cdk8 module could positively regulate transcription by modulating Mediator conformation.


Asunto(s)
Humanos , Quinasa 8 Dependiente de Ciclina , Química , Complejo Mediador , Química , Modelos Moleculares , Conformación Proteica , Subunidades de Proteína , Química , Proteínas de Saccharomyces cerevisiae , Química
SELECCIÓN DE REFERENCIAS
DETALLE DE LA BÚSQUEDA