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1.
Mem. Inst. Oswaldo Cruz ; 109(8): 1064-1069, 12/2014. tab
Artículo en Inglés | LILACS | ID: lil-732595

RESUMEN

In sandflies, the absence of the peritrophic matrix (PM) affects the rate of blood digestion. Also, the kinetics of PM secretion varies according to species. We previously characterised PpChit1, a midgut-specific chitinase secreted in Phlebotomus papatasi (PPIS) that is involved in the maturation of the PM and showed that antibodies against PpChit1 reduce the chitinolytic activity in the midgut of several sandfly species. Here, sandflies were fed on red blood cells reconstituted with naïve or anti-PpChit1 sera and assessed for fitness parameters that included blood digestion, oviposition onset, number of eggs laid, egg bouts, average number of eggs per bout and survival. In PPIS, anti-PpChit1 led to a one-day delay in the onset of egg laying, with flies surviving three days longer compared to the control group. Anti-PpChit1 also had a negative effect on overall ability of flies to lay eggs, as several gravid females from all three species were unable to lay any eggs despite having lived longer than control flies. Whereas the longer survival might be associated with improved haeme scavenging ability by the PM, the inability of females to lay eggs is possibly linked to changes in PM permeability affecting nutrient absorption.


Asunto(s)
Animales , Femenino , Masculino , Quitinasas/inmunología , Sueros Inmunes , Factores Inmunológicos/farmacología , Proteínas de Insectos/efectos de los fármacos , Insectos Vectores/efectos de los fármacos , Phlebotomus/efectos de los fármacos , Quitinasas , ADN Complementario , Digestión/efectos de los fármacos , Conducta Alimentaria , Absorción Gastrointestinal/efectos de los fármacos , Hemoglobinas , Sueros Inmunes/inmunología , Proteínas de Insectos , Insectos Vectores/fisiología , Ratones Endogámicos BALB C , Control de Mosquitos/métodos , Oviposición/efectos de los fármacos , Plásmidos , Phlebotomus/fisiología
2.
Yonsei Medical Journal ; : 22-30, 2009.
Artículo en Inglés | WPRIM | ID: wpr-83536

RESUMEN

Chitin, the second most abundant polysaccharide in nature after cellulose, consist exoskeleton of lower organisms such as fungi, crustaceans and insects except mammals. Recently, several studies evaluated immunologic effects of chitin in vivo and in vitro and revealed new aspects of chitin regulation of innate and adaptive immune responses. It has been shown that exogenous chitin activates macrophages and other innate immune cells and also modulates adaptive type 2 allergic inflammation. These studies further demonstrate that chitin stimulate macrophages by interacting with different cell surface receptors such as macrophage mannose receptor, toll-like receptor 2 (TLR-2), C-type lectin receptor Dectin-1, and leukotriene B4 recepptor (BLT1). On the other hand, a number of chitinase or chitinase-like proteins (C/CLP) are ubiquitously expressed in the airways and intestinal tracts from insects to mammals. In general, these chitinase family proteins confer protective functions to the host against exogenous chitin-containing pathogens. However, substantial body of recent studies also set light on new roles of C/CLP in the development and progression of allergic inflammation and tissue remodeling. In this review, recent findings on the role of chitin and C/CLP in allergic inflammation and tissue remodeling will be highlighted and controversial and unsolved issues in this field of studies will be discussed.


Asunto(s)
Animales , Humanos , Quitina/inmunología , Quitinasas/inmunología , Glicoproteínas/inmunología , Hipersensibilidad/inmunología , Inflamación/inmunología
3.
Southeast Asian J Trop Med Public Health ; 1999 Sep; 30(3): 569-71
Artículo en Inglés | IMSEAR | ID: sea-34572

RESUMEN

Apply recombinant chitinase fusion protein antigen, enzyme-linked immunosorbent assays examined anti-filarial antibodies and evaluated of useful value in serological diagnosis and surveillance of lymphatic filariasis. The test jirds were immunized and infected by chitinase and B. malayi third stage larvae respectively. Functional protein molecular of chitinase was analyzed by SDS-PAGE, Western blot. The result shown that jirds from microfilaremia (mf) and donors with Mf were directly to react with chitinase antigen that positive rate was 100%, but Mf-xt antigen was only 80%. Normal jirds and persons sera from unepidemic control donors all were negative. False positives of 5% and 20% reacted with chitinase and Mf-xt antigens respectively. The results indicate that recombinant chitinase antigen is suitable for detection of active occult or patent lymphatic filariasis with daytime blood samples in residents of endemic areas, is easy to be performed and inexpensive.


Asunto(s)
Animales , Antígenos Helmínticos/inmunología , Western Blotting , Brugia Malayi/enzimología , Quitinasas/inmunología , Filariasis Linfática/sangre , Ensayo de Inmunoadsorción Enzimática/métodos , Estudios de Evaluación como Asunto , Gerbillinae , Humanos , Proteínas Recombinantes de Fusión/inmunología
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