Change in nucleic acids [RNA and DNA] contents, of Musca Domestica, under the effects of neem compounds and DDVP

Nucleic acids [RNA and DNA] contents were estimated in Musca domestica [PCSIR strain] after LD50 treatment of nem compounds [i. e. Margosan [OTM], H - 34, N6 - b] and an organophosphate [DDVP]. RNA and DNA contents were inhibited by all the undertest compounds. Treatment with Margosan - [OTM], H -34 and N6 - b resulted in the inhibition of RNA content upto 23.37%, 23.55% and 33.32% respectively. Whereas the organophosphate, DDVP decreased the RNA content upto 9.67% The effectiveness of the compounds for inhibiting RNA contents may be summarized as follows DDVP < Margosan - O[TM] < H -34 < N6-b. DNA contents also decreased by all the compounds. Inhibition was 19.49% by Margosan - OTM 35.52% by H - 34; 25.02% by N - 6 - b, and 28.82% by DDVP. The effectiveness of the compounds for inhibiting DNA contents may be summarized as follows. Margosan- O[TM]

Studies on the effect of dichlorvos and 2 PAM on rat brain acetylcholinesterase

Organophosphates are powerful, widely used pesticides which distrupt normal cholinergic neural impulse transmission of the central and peripheral nervous system in vertebrates by inhibiting AChE. Effect of three graded doses of Dichlorvos [3. 0, 1.5, 0.75 mg/kg b.w., i.p. daily for 7 days] was evaluated an acetylcholinerte-rase activity of rat brain and spinal cord. A dose response curve was obtained and it was observed that the spinal cord was the most susceptible region. Administration of pyridine - 2 - aldoxime, considerably reactivated the enzyme acetylcholinesterase. Variation in the reactivation of enzyme in the discrete regions of rat CNS suggests that individual regions behave as disparate organs and the extent of toxicity depends both on the region and the dose of Dichlorvos administered