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Indian J Biochem Biophys ; 2001 Aug; 38(4): 253-7
Artículo en Inglés | IMSEAR | ID: sea-27133

RESUMEN

The microsomal fraction from the log phase of Entamoeba histolytica cells contains Ins(1,4,5)P3 and Ins(1,3,4,5)P4 binding activity. The binding proteins/receptors for both Ins(1,4,5)P3 and Ins(1,3,4,5)P4 were purified and found to be specific for each ligand. The molecular masses for native proteins for InsP3 and InsP4 are 138 kDa and 130 kDa respectively having subunits of 69 kDa and 64 kDa respectively. That these proteins are associated with Ca2+ release was confirmed by including these proteins separately in proteoliposomes and adding InsP3 and InsP4 in both the cases.


Asunto(s)
Animales , Sitios de Unión , Calcio/metabolismo , Canales de Calcio/aislamiento & purificación , Membrana Celular/metabolismo , Entamoeba histolytica/química , Inositol 1,4,5-Trifosfato/metabolismo , Receptores de Inositol 1,4,5-Trifosfato , Fosfatos de Inositol/metabolismo , Peso Molecular , Subunidades de Proteína , Proteolípidos/metabolismo , Receptores Citoplasmáticos y Nucleares/aislamiento & purificación
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