A novel combination of plant growth regulators for in vitro regeneration of complete plantlets of guar [Cyamopsis tetragonoloba (L.) Taub.].

A novel combination of plant growth regulators comprising indole-3-butyric acid (IBA), 6-benzylaminopurine (BA) and gibberellic acid (GA3) in Murashige and Skoog basal medium has been formulated for in vitro induction of both shoot and root in one culture using cotyledonary node explants of guar, (Cyamopsis tetragonoloba). Highest percentages of shoot (92%) and root (80%) induction were obtained in the medium containing (mg/L) 2 IBA, 3 BA and 1 GA3. Shoot regeneration from the cotyledonary node explants was observed after 10-15 days. Regeneration of roots from these shoots occurred after 20 to 25 days. The regenerated plantlets showed successful acclimatization on transfer to soil. This protocol is expected to be helpful in carrying out various in vitro manipulations in this economically and industrially important legume.

Efecto de diferentes concentraciones de ácido antranílico en el crecimiento del maíz / The effect of different anthranilic acid concentrations on maize growth

La rizobacteria Azospirillum brasilense basa su capacidad promotora del crecimiento vegetal en la producciónde auxinas y otras hormonas, entre ellas el ácido antranílico (AA). En este trabajo se determinó el efectodel AA (0, 500, 1000, 3000 y 5000 ppm) en el crecimiento y la acumulación de biomasa del híbrido de maíz83G66 en condiciones de invernadero. El AA de 500 a 3000 ppm promovió un mayor crecimiento (área foliary altura de planta), y el AA de 1000 y 3000 ppm incrementó significativamente la biomasa seca en maíz encomparación con el testigo. El 90% de la asimilación del AA en las plantas ocurrió durante los primeros 10días después del tratamiento.

The plant growth-promoting ability of rhizo-bacterium Azospirillum brasilense is based on production of auxins and other hormones, among them outstanding antranilic acid (AA). In this work we determined the antranilic acid (0, 500, 1000, 3000, and 5000 ppm) effects on maize growth and dry biomass accumulation under greenhouse conditions using the hybrid 83G66. From 500 to 3000 ppm of AA significantly increased plant growth (leaf area and plant height) while 1000 and 3000 ppm of AA significantly increased dry biomass accumulation in maize compared with control (0 ppm). The 90 % of AA in maize plants was assimilated during the early 10 days after treatment.

In vitro response of encapsulated somatic embryos of Lagerstroemia indica L.

A method to produce encapsulatable units for synthetic seeds was developed in L. indica. Somatic embryos were harvested from leaf derived embryogenic callus on Murashige and Skoog's basal medium supplemented with 2, 4-dichlorophenoxy acetic acid (2, 4-D, 0.5 mg/l), 6-benzyl amino purine (BAP, 1 mg/l) and ascorbic acid (AA, 50 mg/l). The embryos were encapsulated in alginate beads and dehydrated. Germination ability of the artificial seeds were investigated. The frequency of regeneration from the encapsulated embryos was significantly affected by (i) the concentration of alginate (ii) the duration of storage, and (iii) the effect of different types of media. A 2% sodium alginate concentration on MS salts resulted in significantly higher germination frequencies than at other concentrations. L. indica showed maximum germination on MS medium (93.84%) after 6 weeks of culture. The germinated synthetic seeds with well developed roots and shoots were transferred successfully to green house. This is the first report on artificial seeds in Lagerstroemnia indica.