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1.
Braz. oral res ; 24(4): 413-418, Oct.-Dec. 2010. ilus, tab
Artículo en Inglés | LILACS | ID: lil-569219

RESUMEN

The objective of this study was to evaluate the effect of photodynamic therapy with erythrosine and rose bengal using a light-emitting diode (LED) on planktonic cultures of S. mutans. Ten S. mutans strains, including nine clinical strains and one reference strain (ATCC 35688), were used. Suspensions containing 10(6) cells/mL were prepared for each strain and were tested under different experimental conditions: a) LED irradiation in the presence of rose bengal as a photosensitizer (RB+L+); b) LED irradiation in the presence of erythrosine as a photosensitizer (E+L+); c) LED irradiation only (P-L+); d) treatment with rose bengal only (RB+L-); e) treatment with erythrosine only (E+L-); and f) no LED irradiation or photosensitizer treatment, which served as a control group (P-L-). After treatment, the strains were seeded onto BHI agar for determination of the number of colony-forming units (CFU/mL). The results were submitted to analysis of variance and the Tukey test (p < 0.05). The number of CFU/mL was significantly lower in the groups submitted to photodynamic therapy (RB+L+ and E+L+) compared to control (P-L-), with a reduction of 6.86 log10 in the RB+L+ group and of 5.16 log10 in the E+L+ group. Photodynamic therapy with rose bengal and erythrosine exerted an antimicrobial effect on all S. mutans strains studied.


Asunto(s)
Caries Dental/tratamiento farmacológico , Fotoquimioterapia/métodos , Fármacos Fotosensibilizantes/farmacología , Streptococcus mutans/efectos de los fármacos , Análisis de Varianza , Carga Bacteriana , Biopelículas/efectos de los fármacos , Biopelículas/efectos de la radiación , Células Cultivadas , Eritrosina/farmacología , Rosa Bengala/farmacología , Streptococcus mutans/aislamiento & purificación , Streptococcus mutans/efectos de la radiación , Factores de Tiempo
2.
Indian J Biochem Biophys ; 1990 Aug; 27(4): 248-50
Artículo en Inglés | IMSEAR | ID: sea-27480

RESUMEN

A modification of water oxidation complex in spinach chloroplasts by rose bengal (RB), a known histidine modifying agent, has been studied using thermoluminescence (TL) technique. The changes in the TL profiles at low concentrations of the dye are explained on the basis of alterations in the protein dynamics while those at higher concentrations of the dye are related to the oxidation of histidine residues.


Asunto(s)
Cloroplastos/efectos de los fármacos , Histidina , Mediciones Luminiscentes , Oxidación-Reducción , Fotosíntesis , Rosa Bengala/farmacología , Agua/metabolismo
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