Process for isolating and purifying stigmasterol and sitosterol derived products from glycine max seeds

Two major sterol dos soybean, Glycine max L. [Leguminosae], were isolated with Thin Layer Chromatography [TLC] and argentic TLC. They were examined using NMR, IR and MS instrumental analysis and identified as stigmasterol [stigmast-5, 22-dien-3beta-ol] acetate and sitosterol [stigmast-5-en-3beta-ol] acetate