Applicability of papain solutions in immunohematology / Aplicabilidade de soluções de papaína em imuno-hematologia

ABSTRACT Objective: To compare the enzyme activity of different presentations of papain solution to validate in-house preparations. Methods: Two papain solutions were prepared, and the third presentation was a commercial solution. Tests were carried out with samples of red cells typed as weak RhD. Results: In-house prepared papain solutions showed similar enzyme reactivity, and statistically no differences compared to the enzyme activity of the commercial solution. Conclusion: Evaluating the cost-benefit ratio, the in-house prepared papain solutions present more economic advantages, and can be incorporated into immunohematological routines as a way to cope with periods of financial crisis and cost-containment policies.

RESUMO Objetivo: Comparar a atividade enzimática de diferentes apresentações de solução de papaína para validação de preparados in-house. Métodos: Foram preparadas duas soluções de papaína, e a terceira apresentação tratou-se de uma solução comercial. Os testes comparativos das reações enzimáticas foram realizados com amostras de hemácias tipadas como RhD fraco. Resultados: As soluções de papaína preparadas in-house apresentaram reatividade enzimática semelhante e estatisticamente sem diferenças em comparação com a atividade enzimática da solução comercial. Conclusão: Avaliando-se a relação entre custo e benefício, as soluções de papaína preparadas in-house são economicamente vantajosas, podendo ser incorporadas às rotinas imuno-hematológicas como forma de enfrentamento em períodos de crise financeira e em políticas de retenção de gastos.

Detection of Rh antibodies using two low ionic diluents: extension of the incubation time and the number of Rh antibodies detected.

BACKGROUND: Low ionic strength solution (LISS) is used to increase the rate of association of antibody to the corresponding antigen during antibody detection tests. A number of LISSs are available on the market. AIMS: The efficiency of two commercial low ionic diluents, DiaMed ID-CellStab and Inverclyde LISS were assessed using the DiaMed-ID LISS Coombs microtube column system and an incubation time varying from 15 to 35 min. MATERIALS AND METHODS: One-hundred samples containing five Rh antibodies (anti-D, anti-C, anti-E, anti-c and anti-e) were tested against commercial red cells using the two low ionic diluents after 15, 25 and 35 min. RESULTS: The Inverclyde LISS detected 91, 95 and 96% of the Rh antibodies compared to 78, 79 and 83% for ID-CellStab after 15, 25 and 35 min incubation time, respectively, for both methods. CONCLUSIONS: The Inverclyde LISS is a more suitable and efficient diluent than ID-CellStab for the detection of Rh antibodies. The sensitivity of Rh antibody detection increased for the both methods as the incubation time increased.

To estimate blood quality and blood preservative capacity by international standard solutions and S.NA.G solution at 4?20C

Analysis of 30 IU of total blood, in which 15 IU of professional blood donors and 15 IU of voluntary blood donors. 20 IU of erythrocyte volume, of them 10 IU were preserved in CPD/S, A, G, M2 (terumor) solutions and 10 IU were preserved in S, Na, G (glucoza 5%, NaCl 9%o with rate of 1:2). The results showed that: the erythrocyte indexes in the blood IU of the volutary blood donors were significantly higher compared to that of the professional blood donors. There was no significant differences of the erythrocyte indexes in blood IU preserved in the international standard solutions until the date of 10, 15, 20 and 30th and in S, Na, G solutions after producing and at the date of 10th at 4  2oC. However, erythrocyte indexes were found to be different in blood IU preserved in S, Na, G at the date of 15th and the following time

Método pratico para o preparo de soluçöes à base de adrenalina / Functional method for preparation of solutions using adrenaline

Método prático de preparo de soluçöes de adrenalina, à concentraçäo desejada, partindo de um simples processo de diluiçäo