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1.
Artículo en Inglés | IMSEAR | ID: sea-17873

RESUMEN

BACKGROUND & OBJECTIVES: Group A Streptococcus, causative agent of several clinical manifestations codes for multiple protein invasins which help the bacterium to enter non-phagocytic cells. C5a peptidase (SCPA) is a surface protein conserved among different serotypes of M1 strain. The present study was taken up to study SCPA promoted fibronectin independent entry of GAS into epithelial cells. METHODS: An isogenic 90226 emm1deltaAB (M1(-)) mutant was constructed with thermosensitive pGhost vector. This isogenic M1(-) mutant expressed SCPA on the surface as determined by Western blotting and immunofluorescence. RESULTS: On preincubation with anti-SCPA serum, the isogenic M1(-) strain exhibited 54 per cent decreased invasion as compared to the bacteria incubated with control serum. Also, purified recombinant SCPA proteins blocked internalization of M1(-) streptococci into HEp-2 cells. The M1(-) strain invaded at the same efficiency in the presence or absence of fibronectin. INTERPRETATION & CONCLUSION: These results suggested that SCPA acted as a potential invasin of group A streptococcus and promoted invasion independent of fibronectin.


Asunto(s)
Adhesinas Bacterianas/metabolismo , Western Blotting , Línea Celular , Endopeptidasas/metabolismo , Células Epiteliales/microbiología , Fibronectinas/fisiología , Humanos , Streptococcus pyogenes/fisiología
2.
Artículo en Inglés | IMSEAR | ID: sea-16225

RESUMEN

Strains of 12 T types of group A streptococci preserved by sand desiccation and stored at 4 degrees to 10 degrees C were regularly sub-cultured to check their viability. For this, streptococci coated onto sand particles mixed with sterile sheep blood were inoculated into Todd-Hewitt broth with added blood and incubated for 24 to 48 h. Checking for viability every six months showed that group A streptococci could be preserved by this method for 21 yr. Our study shows that sand desiccation is a convenient and cheap method for the long-term preservation of streptococci in laboratories where other methods of preservation are not feasible on a regular basis.


Asunto(s)
Técnicas Bacteriológicas , Supervivencia Celular , Criopreservación , Desecación , Preservación Biológica/métodos , Dióxido de Silicio , Streptococcus pyogenes/fisiología
3.
Rev. microbiol ; 20(1): 10-1, jan.-mar. 1989.
Artículo en Portugués | LILACS | ID: lil-75266

RESUMEN

Lisogenia em amostras de estreptococos do grupo A foi examinada. Verificou-se que entre as lisogênicas a resistência a tetraciclina era mais comum


Asunto(s)
Streptococcus pyogenes/fisiología , Tetraciclina , Lisogenia , Antibacterianos , Farmacorresistencia Microbiana , Gentamicinas , Ampicilina
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