Primer registro de Talaromyces udagawae relacionado con cuerpos humanos en descomposición, en suelo de la Argentina / First record of Talaromyces udagawae in soil related to decomposing human remains in Argentina

The morphologic features of Talaromyces udagawae Stolk and Samson are here described and illustrated. This teleomorphic Ascomycota fungus was isolated from soil obtained in Buenos Aires province (Argentina) from beneath a human cadaver in an advanced state of decomposition. After washing and serial dilution of the soil along with moist-chamber techniques for fungal cultivation, T. udagawae formed very restricted colonies of bright yellow color on different growth media with 8-ascospored asci. The ascospores were ellipsoidal and ornamented. The anamorphic state was not observed. Molecular-genetic techniques identified the species. The present record is the first of the species in Argentina, pointing it as a tool to identify soils where cadaver decomposition occurs.

Se describen e ilustran las características morfológicas de Talaromyces udagawae Stolk y Samson. Se aisló el estado teleomórfico de este hongo Ascomycota de suelo obtenido en la provincia de Buenos Aires (Argentina), por debajo de un cadáver humano en avanzado estado de descomposición. Las muestras de suelo fueron analizadas mediante lavado, dilución seriada y cámaras húmedas, técnicas ampliamente usadas para el estudio de hongos de suelo. T. udagawae formó colonias muy restringidas de color amarillo brillante en diferentes medios de cultivo, con ascos con 8 ascosporas. Las ascosporas eran elipsoidales y ornamentadas. No fue hallado el estado anamórfico. La especie también fue identificada mediante técnicas moleculares. El presente registro es el primero de la especie en la Argentina y el único que la postula como herramienta para identificar suelos donde ocurre una descomposición cadavérica.

Production, partial purification and some properties of alpha-L-arabinofuranosidase from chaetomium thermophile and talaromyces thermophius

The PRODUCTION of extracellular alpha-L-arabinofuranosidase [ABFase], partial purification and some of its properties by locally isolated strains of Chaetomiuin thermophile and Talaromyces therinophihis were studied. The best static culture conditions for the enzyme production were 2% sugar beet pulp; pH 5.0 and 45°C for both strains. The best nitrogen source is ammonium sulphate for C. thermophile and yeast extract for P thermophile us. The enzyme was partially purified from the culture supernatant by precipitation with ammonium sulfate treatment, gel filtration on Sephadex G[100]with purification fold 1589 and 8.32 for C. thermophile and T thermophilus, respectively. The purified enzyme of both fungi displayed an optimal activity at 50°C. The enzyme was stable at temperatures between 30-70°C. The optimum pH for C. thermophile was 5.5 and the enzyme was stable at pH between 3.0 - 6.5. While the Optimum pH for T. thermophilus was 6.5 and the enzyme was stable at pH between 3.0-7.5