RESUMEN
An effective preservation method and decreased rejection are essential for tracheal transplantation in the reconstruction of large airway defects. Our objective in the present study was to evaluate the antigenic properties of glycerin-preserved tracheal segments. Sixty-one tracheal segments (2.4 to 3.1 cm) were divided into three groups: autograft (N = 21), fresh allograft (N = 18) and glycerin-preserved allograft (N = 22). Two segments from different groups were implanted into the greater omentum of dogs (N = 31). After 28 days, the segments were harvested and analyzed for mononuclear infiltration score and for the presence of respiratory epithelium. The fresh allograft group presented the highest score for mononuclear infiltration (1.78 ± 0.43, P <= 0.001) when compared to the autograft and glycerin-preserved allograft groups. In contrast to the regenerated epithelium observed in autograft segments, all fresh allografts and glycerin-preserved allografts had desquamation of the respiratory mucosa. The low antigenicity observed in glycerin segments was probably the result of denudation of the respiratory epithelium and perhaps due to the decrease of major histocompatibility complex class II antigens.
Asunto(s)
Animales , Perros , Femenino , Masculino , Crioprotectores , Glicerol , Rechazo de Injerto/prevención & control , Mucosa Respiratoria/inmunología , Tráquea/trasplante , Trasplante Heterotópico/inmunología , Criopreservación/métodos , Rechazo de Injerto/inmunología , Rechazo de Injerto/patología , Epiplón/cirugía , Preservación de Órganos/métodos , Mucosa Respiratoria/patología , Tráquea/inmunología , Tráquea/patología , Trasplante Heterotópico/patologíaRESUMEN
El embarazo heterotópico es una entidad rara caracterizada por embarazo ectópico asociado a embarazo intrauterino. Su mayor incidencia se origina en la práctica creciente de la fertilización asistida. Aunque sobre este procedimiento se ejerce un adecuado seguimiento, ello no garantiza un diagnóstico fácil y oportuno