Establishment of rat heart-kidney insomnia model consistent with traditional Chinese medicine syndrome and its serum metabolomics / 中国中药杂志

Enzyme-linked immunosorbent assay(ELISA) and metabolomics were used to analyze and compare two animal models of heart-kidney insomnia, in order to explore a more ideal animal model and preliminarily explore the essence of heart-kidney insomnia. Based on the clinical symptoms and disease characteristics of heart-kidney insomnia, the animal model of heart-kidney insomnia was reproduced through intraperitoneal injection with p-chlorophenylalanine(PCPA) and multi-factor interaction. The animal model of disease-syndrome combination was evaluated by behavioral observation, ELISA and metabolomics. Wistar rats were randomly divided into normal group, PCPA group and compound model group(FH). The rats' behavior, body weight, adrenal index and spleen index were recorded. The levels of corticotropin releasing hormone(CRH) and adrenocorticotropin(ACTH) in serum were detected by ELISA, and the differential metabolites in serum were detected by UPLC-QE-MS. The body weight and adrenal index in FH group were significantly lower than those in PCPA group(P<0.05); whereas ACTH and CRH in FH group were significantly higher than those in PCPA group by ELISA; nine potential biomarkers were identified by serum sample statistics. There were four main metabolic pathways in cardiorenal insomnia: pentose phosphate metabolism, alanine, aspartic acid and glutamic acid metabolism, histidine metabolism, and taurine and subtaurine metabolism. PCPA and multi-factor interaction method can successfully replicate the insomnia model, but multi-factor modeling method is more similar to clinical traditional Chinese medicine syndrome. Animal behavior, ELISA and metabolomics were used to evaluate the rat model of cardiorenal insomnia from in vitro to in vivo, from macro to micro, and from individual to the whole.

Queixas de insônia e menopausa entre funcionárias de uma universidade no Rio de Janeiro: estudo pró–saúde / Insomnia complaints and menopause among female employees at a university in Rio de Janeiro: the pró-saúde study

Estudos demonstram que mulheres na perimenopausa e pós-menopausa apresentam maior latência para o sono, dificuldade de manutenção e estão menos satisfeitas com a qualidade do sono quando comparadas àquelas que não estão na menopausa. Apesar da vasta literatura sobre a insônia na menopausa, diversos aspectos ainda são controversos ou não foram suficientemente estudados. Objetivos: Artigo I: Estimar a prevalência de insônia e avaliar sua associação com o status menopausal em uma população de mulheres. Artigo II: Entre mulheres na menopausa, investigar a associação entre fatores psicossociais, morbidade física e mental, características da menopausa e queixas de insônia. Métodos: Artigo I: Foi realizado um estudo seccional entre funcionárias de uma universidade no Rio de Janeiro participantes de um estudo longitudinal (Estudo Pró-Saúde)(n=2.189), onde foi utilizado um questionário autopreenchível para avaliação de insônia, status menopausal e demais variáveis de estudo. Foram consideradas como “em menopausa”as mulheres não-grávidas que responderam que não ficavam mais menstruadas (peri e pósmenopausa). A insônia foi analisada como dificuldade em iniciar o sono, dificuldade em manter o sono e queixa geral de insônia (combinação das anteriores). Os Odds Ratios (OR) brutos e ajustados foram calculados através de regressão logística multivariada.

Viewing humorous film improves nighttime wakening in children with atopic dermatitis.

INTRODUCTION: Patients with atopic dermatitis suffered from night-time wakening, but the exact mechanism of it was not known. Ghrelin was involved in growth hormone secretion, regulation of appetite, anxiety,night-time wakening and stress. METHODS: Thus salivary ghreli levels during the night were measured in 40 healthy children or 40 patients with atopic dermatitis with night-time wakening. Salivary ghrelin levels at 02:00 h were markedly elevated in patients with atopic dermatitis compared to those in healthy children. RESULTS: Neither viewing control non-humorous film nor viewing humorous film had any effect on healthy children. In contrast, viewing humorous film improved night-time wakening and reduced elevation of salivary ghrelin levels in patients with atopic dermatitis, while viewing control film failed to do so. CONCLUSION: Viewing humorous film may be useful in the treatment of night-time wakening in patients with atopic dermatitis.