Résumé
Background: Antimicrobial peptides with relative length [2-100 amino acids] and positive charge [pure charge [+9]-[+2]] are amphiphilic that isolated from a wide range of animals. Recently, these peptides have been known as a part of innate immune response. Nowadays, more than 500 antimicrobial peptides from animals have been reported
Objectives: The aim of this study was to evaluate the anti-Candida effects of cationic peptides derived from Rana ridibunda skin
Methods: In this study, using alcohol-acid technique, peptides of frog's skin were isolated and purified by Sep-Pack and Sephadex column. Then the anti-Candida activity [Fluconazole Resistance C. albicans, Fluconazole Sensitive C. albicans, and C. glabrata] of the peptides in different concentrations were evaluated
Results: Regarding to statistical analysis, peptides in concentration ranging from 25 to 100 micro g/ml had the most anti-Candida activities. In respect to different understudy Candida species, these agents had the less effect on the Fluconazole Resistance C. albicans [p<0.05]
Conclusions: The anti-Candida effects of cationic peptide obtained from frog skin are approved in this study
Résumé
Subclinical ketosis can cause greater economic loss due to a lack of clinical symptoms. The present study was aimed to design a chemical method for measuring serum ketone bodies in the affected subclinical cows. Acetoacetate concentrations were measured using a nitroprusside reaction and beta-hydroxy butyric acid [BHBA] which was oxidized to acetoacetate using nanosilver particles to determine its concentration. Recovery tests were done for different concentrations of betahydroxy butyrate in bovine pooled serum. Actoacetate levels were in range of 0.1-6 mM, and the values for BHBA were found to be in the range of 0.125-3 mM. It was concluded that this technique can be considered as a simple method for measuring ketone bodies in biological fluids