Preparation and quality control of 153Sm-[tris[1, 10 -phenanthroline] samarium [III]] complex

The 153Sm-[tris[1, 10-phenanthroline] Samarium[III]]complex [153Sm-PL3] was prepared in view of development of targeting therapeutic compounds for malignancies, and interesting in-vitro anti-tumor activities of lanthanide phenanthroline complexes,. Sm-153 chloride was obtained by thermal neutron flux [4 × 1013 n.cm- 2.s-1] of enriched 152Sm2O3 sample, dissolved in acidic media. The labeling was performed in ethanol in 24h, controlled by ITLC [1.0mM DTPA, pH.5, as mobile phase]. The partition coefficient for the labeled compound was also determined. Results: A radiochemical yield of more than 95% was obtained. Radiochemical purity of 96% was obtained using ITLC with specific activity of about 27.75 GBq/mg. The radio-labeled complex was stable in aqueous solution at least 24 hours and no significant amount of free 153Sm was released from the complex. The partition coefficient for the labeled compound was determined [log P. 3.4]. The complex was stable in final formulation for 66h. The biological evaluation of the compound is under investigation. The radiolabeled compound used in this study was a very inexpensive and useful agent for the use as a therapeutic compound

Development of [201T1][III] oxinate complex for in vitro cell labeling

The incorporation of thallium-201 into 8-hydroxyquinoline was targeted for cell labeling due to interesting physical properties and wide availability of this nuclide as a single photon emission computed tomography [SPECT] radionuclide. Thallium-201 [T[1/2]=3.04 d] in Tl[+] form was converted to Tl[3+] cation in presence of O[3]/6M HCl and di-isopropyl ether, controlled by radiothin layer chromatography [RTLC] /gel electrophoresis methods. The final evaporated activity reacted with ethanolic 8-hydroxy-quinoline [oxine] solution in normal saline to yield [[201]Tl][III] oxinate at room temperature after 0.5 h, followed by solid phase extraction/purification using C18 Sep-Pak column and partition coefficient determination for water/lipid solubility. In vitro red blood cell [RBC] labeling was also performed. A radiochemical yield of more than 95% was obtained. Radiochemical purity of 92% was obtained using RTLC [>90% using HPLC] with specific activity of about 820 GBq/mmol. The tracer was stable in the final product and in presence of human serum at 37°C up to 6h. The partition coefficient of lopP=5.5 was obtained. The labeled compound was used in RBC labeling. The cell uptake ratio was 0.47 after 240 min. [[201]Tl][III] oxinate used in this study is a widely available agent for use in RBC labeling studies in biology, medicine and various other research areas

Monthly variations of urinary stone colic in Iran and its relationship to the fasting month of Ramadan

Objective: To compare the prevalence of Renal Colic [RC] in Ramadan [the month of fasting for Moslems] with other months of the lunar year

Material and Methods: Records of 574 subjects, who were admitted in the two medical centers of Varamin [a city in a hot region of Iran], were reviewed in order to estimate the time trend of RC

Results: This study included 398 males [69.3%] and 176 females [30.7%]. Twenty-seven males [62.8%] and 16 females [37.2%] were admitted in Ramadan; and 371 males [69.9%] and 160 females [30.1%] in other months [p<0.4] of the year. RCs were more common in June [68 patients, 11.8%], July [65 patients, 11.3%] and November [60 patients, 10.5%]. Forty-three subjects [7.5%] admitted in Ramadan; the frequency was not significantly different from mean admission of the year [48.3 +/- 17 patients]. There was also no significant difference between frequency of admissions in Ramadan and mean admission during cold half of the year [36.8 +/- 18.34 patients, p = 0.3]. Mean admission [64.4 +/- 3.3 patients] in warm seasons were significantly higher than Ramadan [p < 0.001]

Conclusion: Lack of difference in the two groups indicates that higher temperature rather than fasting as a cause for RCs

[Radiological, clinical and paraclinical study of iohexol in myelography of the dog]

Myelography and analysis of cerebrospinal fluid are frequently required for the diagnosis of central nervous system diseases. Residual effects of radiographic contrast media may alter interpretation of the C.S.F if a sample is analyzed shortly after myelography. Iohexol is a water - soluble, nonionic radiographic contrast medium that has widespread acceptance in myelography. In the present study the effects of iohexol myelography and repeated cisternal puncture in the dog were studied. Ten clinically normal mixed breed dogs prepared for aseptic puncture of the cisterna cerebello medullaris. Samples were collected for cytologic and chemical analysis. Blood samples for a hemogram were also collected at this time. Six dogs then received an intracisternal injection of a sterile solution of iohexol [omnipaque], 0.45 ml/kg body weight. C.S.F. was obtained from the four control dogs, but they received no iohexol. By the same aseptic technique C.S.F. was obtained form each dog 24 hours after injection for cytologic and chemical analysis. Blood samples were also collected at this time. Factors of total protein, glucose, creatine phosphokinase, physical specifications, cell count and differentiation of C.S.F from both groups before and 24 hours after the injection of contrast media were determined. Other factors like hematocrite, total protein glucose, red and white cell count of serum and peripheral blood of animals before and 24 hours post injection of contrast media were determined as well. The animals were followed up for 27 hours all the changes in temperature, respiration and heart rate during these hours and the anesthetic condition in two steps of examination were not significant and there was no any seizeures, The mean of total protein concentration in C.S.F of the test group, after 24 hours of myelography, was increased significantly [p<0.05]. The mean of total cell count, red and white cell count in the C.S.F of the test group 24 hours after the myelography showed a significant increase [p<0.01]. No significant changes were found for any other parameter. The myelographs had an appropriate quality