Hairy root cultures of hypericum perforatum l.; a promising method for the high scale production of hypericin

Background: biotechnologic methods are common for secondary metabolites production from the plants and other sources in pharmaceutical sciences. Hairy root cell lines as the biotechnologic method have been used for in vitro production of major plant metabolites

Objective: in this study, hairy roots of Hypericum Perforatum have been prepared using the seeds and bio transformed by bacteria. Finally, the hypericin have been producted by the hairy roots

Methods: first, the seeds have been incubated in the plant media to hairy roots produced. Then, the hairy roots have been dipped in Rhizobium rizogenes suspension for biotransformation of bacterial genes. Morphological and phytochemical features of hairy roots have been determined in order to select the H. perforatum genotypes with higher hypericin contents. The fresh and dry weight of ten lines clones were measured after 30 days. In addition to, methanolic extracts of final hairy roots have been prepared and hypericin has been isolated and assayed by High Performance Liquid Chromatography

Results: according to the observations, one of ten hairy root lines shows 75-fold higher hypericin content [339.27 ppm] compared to non-transformed H. perforatum [4.58 ppm] in the same of body weight of roots. The clone with the most content of hypericin had significant development of biomass of hairy roots and increase the hypericin production. Transformed clones were varied in morphology, growth, and metabolite productivity

Conclusion: the mentioned methods induce the production of hairy root secondary metabolites in high scale to improve the quality and the quantity of pharmaceutical compounds

Anesthetic management of diaphragmatic hernia repair in a dog: a case report and literature review of anesthetic techniques

This case report describes the anesthetic management and ventilation technique in the surgical treatment of traumatic diaphragmatic hernia in a dog. A 5-month-old 8-kg female terrier with a history of car accident was presented for femoral fracture repair. Before anesthetic induction, marked tachypnea and dyspnea were noted. Diaphragmatic hernia was diagnosed based upon radiographic and ultrasonographic findings

Exploratory laparotomy revealed diaphragmatic rupture and herniation of spleen, omentum, parts of liver lobes and stomach into the thoracic cavity. The importance of thorough physical examination and patient assessment, anesthetic management and monitoring, provision of adequate ventilation and oxygenation during surgery using standard ventilation equipment are discussed?

Investigation of environmental health condition and safety of schools in kerman in 2007

There is a vast variety of environmental health condition factors influencing students' health status such as the quality and quantity of drinking fountains, toilets, potable water condition, removing sewage system, garbage management, safety, sport grounds arid green area. Lack of information about the conditions of these factors may leave devastating and irreparable effects on the health condition of society. Regarding the important role of school environmental conditions in promoting the health level in society, this research was conducted in Kerman in 1386 to have an overall investigation of school health condition and school agronomy. This is a descriptive, cross- sectional research. The sample included 257 students of primary, secondary, high school and pre-university in Kerman. The data were collected by census.The checklist was cmpleted by going to schools, observations and interviews with practitioners. Later the collected data were analyzed with SPSS software. Of the schools, 68% were state-run whereas 32% were non- profitable schools. In 55.7% of primary and secondary schools the required area per student had been observed while it hadnot been observed in 86% of high schools and technical schools.The estimated green area per student is 0.5 m2 but had not been observed in 67.4% schools. 53.1% schools had no health service room. 89.9% of schools had healthy potable water. In only 66.3% of schools each toilet was provided for 40 students. In 95.96% of schools sewage removing system was hygienic. 76% of schools had hygienic dust bin among which 90.2% followed the standard time lag of empting and washing the dust bins. 76% of schools observed the article No. 13 of school legislation for nutritious, cosmetic and hygienic materials in schools buffet. In 92.6% of schools, there was a proportion between benches and students height. 80% of school took advantage of sunlight. It seems that during the last 13 years, the health and safety conditions of schools have developed and improved and, generally speaking, 80% of the schools had made use ed light perfectly