new flavonol glycoside from polygonum bellardu all: growing in Egypt

A new flavonol glycoside named myricetin-3-O-[5"-acetyl alpha-arabinofuranoside] has been isolated from Polygonum bellardii All F. Polygonaceae growing in Egypt, together with quercetin-3-O-[5"-acetyl alpha -arabinofuranoside], quercetin-3-O-rutinoside [rutin], alpha-amyrin, beta sitosterol and beta-sitosterol-3-O-beta glucoside. Their structures were elucidated using different spectral techniques

Xanthones from cell cultures of hypericum gnidioides seem

Oxygenated and prenylated xanthones were isolated from the cell cultures of Hypericum gnidioides Seem. when grown in modified B5 medium in the dark. Based on the spectral methods, the structure of the isolated compounds were elucidated as 1,7-dihydroxyxanthone [euxanthone], 1,3,7-trihydroxyxanthone, 1,3,5,6-tetrahydroxyxanthone, 1,3,6,7-tetrahydroxy-8-[3-methylbut-2-enyl] xanthone and 1,3,6,7-tetrahydroxy-2,8-di[3-methylbut-2-enyl] xanthone [gamma-mangostin]. The occurrence of both 1,7-dihydroxyxanthone and 1,3,7- trihydroxyxanthone which is recorded for the first time in cell cultures of Hypericum species indicate the presence of a reductase activity responsible for eliminating the 3-hydroxy group and confirm the biosynthetic pathway of xanthones in Hypericum species respectively

Biotransformation of Flavonols to Flavonols-3-O-glucoside in cell cultures of astragalus sieberi DC

One distinct glucosyltransferase [GT] has been partially purified and characterized from cell cultures of Astragalus sieberi DC.; Family Leguminosae. Callus cultures were established from shoots of sterile germinated seeds maintained on solid MS medium supplemented with 4.5 microM 1-naphthylacetic acid [NAA] and 2.3 microM kinetin [KIN]. The cell suspension cultures were obtained by transport of callus cultures to liquid MS medium with the same hormone supplementation. The GT was found to exhibit maximum activity at pH 7.5 and an incubation temperature of 35°. The preferred substrate of GT was found to be kaempferol, the second best substrate was quercetin. The isolated enzymatic products were detected by TLC and HPLC and identified by spectral analysis and comparison with authentic compounds.This experiment from economic point of view provides the best conditions for large scale production of glucosides of kaempferol, quercetin and isorahmnetin

Phenylethanoid glycosides from barleria cristata L. callus cultures

Three phenylethanoid glycosides viz; Beta-[[3', 4'-dihydroxyphenyl]-ethyl]-[4"-O-caffeoyl]- Beta- D-glucoside [desrhamnosylacteoside] [1], Beta-[[3', 4'-dihydroxyphenyl]-ethyl]-[3"-O-Lrhamnosyl]-[4"-O-caffeoyl]-Beta-D-glucoside [acteoside] [2] and Beta-[[3', 4'-dihydroxyphenyl]- ethyl]-[3", 6"-O-L-dirhamnosyl]-[4"-O-caffeoyl]-Beta-D-glucoside [poliumoside] [3] were isolated and identified from the callus cultures of Barleria cristata L. The structures of the isolated compounds were established by spectroscopic evidence [UV, 1D and 2D-.1-NMR and ESIMS], further confirmation has been done by comparison with authentic samples. The amount of compounds 1-3 were determined in the callus culture using HPLC

Macro- and micromorphology of leaf stem and root of poylgenum bellardii all growing in Egypt

The macro- and microrphological characters of the leaf stem, and root of Polygonulm bellardii All. growing in Egypt are presented with the aim of finding out the charactrers by which the plant could be identified in both the entire and powdered forms