Prognostic significance of P27 [Kip 1] and MUC1 in papillary transitional cell carcinoma of the urinary bladder

To examine p27 [Kip 1] and MUC1 expression in specimens of papillary transitional cell carcinoma [PTCC] of the urinary bladder and to correlate their expression with the tumor grades,stages and outcome. Paraffin sections from previously diagnosed PTCC bladder were graded, staged and the patients were followed up for 5 years. Ten non-neoplastic urological lesions diagnosed as polypoid cystitis were taken as control. Three sections of 4 um thickness were obtained from every case. One was hematoxylin and eosin [H and E] stained for diagnosis, reviewing and confirmation. The other two sections were immunohistochemically stained for both p27and MUC1. The data of immunohistochemical results were correlated with the following conventional prognostic variables: tumor grade, stage, distant metastasis and 5 year survival. The results showed a highly significant and an insignificant relationship between p27 expression and tumor grade and stage [P<0.01 and P>0.05], respectively. Correlating p27 expression with distant metastasis and overall survival showed a significant relationship with distant metastasis [P<0.05] and a highly significant one with overall survival [P<0.01]. The results showed also a significant relationship between MUC1 expression and both tumor grade [P<0.01] and overall survival [P<0.05]. p27 and MUC1 immunohistochemistry augment the classic histochemistry for the prognosis of PTCC of the bladder as well as improving the prediction of the patient outcome and survival

Role of Helicobacter pylori [H.pylori] infection and cyclooxygenase-2 [COX-2] expression in chronic gastritis and gastric carcinoma: an immunohistochemical study

Malignant tumors of the stomach are common, but the incidence of stomach cancer varies from country to another, probably as a result of genetic, epigenetic and environmental factors. Stomach cancer often occurs in older people whose stomachs produce only small quantities of acid. Although infection with Helicobacter pylori has been proven beyond doubt in the aetiopathogenesis of various gastric disorders, not much is known about the role of H.pylori infection in onset and progression of chronic gastritis as well as gastric cancer. Although recent studies have indicated that the clinicopathological parameters in patients with gastric carcinoma, the prognosis of advanced cancer still remains unsatisfactory. This study aimed at investigating the expression of H pylori antibody and cyclooxygenase-2 [COX-2] in cases with chronic gastritis and gastric carcinomas and to correlate this expression with various clinicopathological parameters. Paraffin sections from previously diagnosed chronic gastritis [CG] and gastric carcinomas [GC] were classified, graded and staged according to the updated for CG and the World Health Organization [WHO], for GC. Two sections were immunohistochemically stained for antibodies against H.pylori and COX-2. One section was stained with Feulgen stain for assessment of ploidy and proliferative activity using the Image analyzer system CAS 200. Infection with H.pylori and cox-2 overex-pression are common in patients with chronic gastritis and gastric carcinoma. Both anti-H pylori and cox-2 proteins are implicated in gastric carcinogenesis and their over expression may be a good predicator for worse prognosis and poor patient's outcome

Role of P27 [Kip 1] and MUC1 in papillary transitional cell carcinoma of the urinary bladder

To examine P27 and MUC1 protein expression in specimens of papillary transitional cell carcinoma-urinary bladder and to compare both of them with the tumor outcome. Paraffin-sections from previously diagnosed papillary transitional cell carcinoma-urinary bladder were graded, staged and followed up for 5 years. Ten non neoplastic urologicl lesions diagnosed as polypoid cystitis were taken as a control. Three sections of 4 micron thickness were obtained for every case. One is H and E stained for diagnosis reviewing and confirmation. The other 2 sections are immunohistochemically stained for both P27 and MUC1. The data of immunohistochemical results were correlated with the following conventional prognostic variables: tumor grade, stage, distant metastasis and 5-year survival of papillary transitional cell carcinoma [PTCC]-urinary bladder. The results showed a highly significant and an insignificant relationships between P27 expression and tumor grade and stage respectively [p<0.01 and >0.05]. Correlating P27 expression with distant metastasis and overall survival showed a significant relationship with distant metastasis [p<0.05] and a highly significant one with overall survival [p<0.01].The results showed also a significant relationships between MUC1 expression and both tumor grade and 5-year overall survival [p<0.05 for both]. In conclusion, P27 and MUC1 immunohistochemistry may augment the classic histochemistry for prognosis of PTCC as well as prediction of the patient outcome. Specific oncogene markers are more significant than oncogene phenotype markers in clinical diagnosis and screening of PTCC