Résumé
OBJECTIVE@#To explore anti-inflammatory activities of organic extract and its semi-purified fractions (ethanol, acetone, methanol/dichloromethane) from the Mediterranean gorgonian Eunicella singularis.@*METHODS@#The anti-inflammatory and analgesic activities were evaluated, using the carrageenan-induced rat paw edema model and the acetic acid writhing test in mice. The gastroprotective activity was determined using HCl/EtOH induced gastric ulcers in rats. The purification and structure elucidation of compound(s) from the more effective fraction were determined by chromatographic and spectroscopic methods and in comparison with data reported in the literature.@*RESULTS@#The fraction F-EtOH showed an important anti-inflammatory activity associated with significant analgesic and gastroprotective properties. The purification and structure elucidation of compound(s) from this fraction lead to the identification of one diterpenoid and four sterols.@*CONCLUSIONS@#These results suggested that components from the active fraction can be used to treat various anti-inflammatory diseases.
Résumé
Objective: To explore anti-inflammatory activities of organic extract and its semi-purified fractions (ethanol, acetone, methanol/dichloromethane) from the Mediterranean gorgonian Eunicella singularis. Methods: The anti-inflammatory and analgesic activities were evaluated, using the carrageenan-induced rat paw edema model and the acetic acid writhing test in mice. The gastroprotective activity was determined using HCl/EtOH induced gastric ulcers in rats. The purification and structure elucidation of compound(s) from the more effective fraction were determined by chromatographic and spectroscopic methods and in comparison with data reported in the literature. Results: The fraction F-EtOH showed an important anti-inflammatory activity associated with significant analgesic and gastroprotective properties. The purification and structure elucidation of compound(s) from this fraction lead to the identification of one diterpenoid and four sterols. Conclusions: These results suggested that components from the active fraction can be used to treat various anti-inflammatory diseases.
Résumé
Seaweeds have caused an emerging interest in the biomedical area, mainly due to their contents of bioactive substances which show great potential as anti-inflammatory, anti-microbial, anti-viral and anti-tumoral drugs. Despite the diversity in quality and quantity of the Mediterranean Tunisian coast flora, with its large contains of marine organisms and seaweeds, most of them have not yet been investigated for pharmacological and biological activities. Anti-oxidant, anti-inflammatory and anti-proliferative effects of the aqueous extracts [AQ] of three brown seaweed respectively, Cystoseira crinita [AQ-C[cri]], Cystoseira sedoides [AQ-C[sed]] and Cystoseira compressa [AQ-C[com]] were investigated. Anti-oxidant activity was evaluated using the DPPH assay. Total phenolic contents were measured using Folin-Ciocalteu method. The anti-inflammatory activity of these extracts was determined in-vivo, using carrageenan induced rat paw oedema assay. The anti-proliferative activity was studied on normal cells [MDCK and rat fibroblast] and cancer [A549, MCF7 and HCT15] cell lines by the ability of the cells to metabolically reduce MTT formazan dyes, in comparison to a reference drug theCisplatin. Results demonstrated that AQ-Ccri, AQ-C[sed] and AQ-C[com]extracts exhibited significant radical scavenging activity. AQ-C[com] extract had the highest total phenolic content. AQ-C[cri], AQ-C[sed] and AQ-C[com] extracts exhibited significant anti-inflammatory activity in a dose dependent manner by comparison to reference drugs. Moreover, AQ-C[cri], AQ-C[sed] and AQ-C[com] extracts showed an important anti-proliferative activity against both Human tumor cell lines HCT15 and MCF7. These pharmacological efficacies of these AQ- extracts of Cystoseira were positively correlated with their total phenol content and their good anti-oxidant activity. The purification and the determination of chemical structures of compounds of these active aqueous extracts are under investigation. It could have a promising role in the future medicine and nutrition when used as drug or food additive