Comparison between S-Allyl cysteine and gliclazide in lowering the blood glucose levels in diabetic rats

Backgroung: Diabetes Mellitus is a global problem caused by the deficiency of Insulin secretion either absolute or relative. Treatment options are Insulin and oral glucose lowering drugs. Current study was designed to find out the glucose lowering properties of S-allylcysteine a garlic derivative sulfur containing amino acid

Objective: To compare blood glucose lowering effects of S-allylcysteine with gliclazide in diabetic rat model

Study Design, Place and Duration: This 3 months animal study was carried out in animal house of ISRA University Hyderabad from August 2014 to Oct 2014

Material and Methods: 32 Male Wistar Rats were randomly divided into 4 equal groups A, B, C and D after selection through non-probability purposive sampling. Group A and B served as positive and negative controls respectively. Diabetes was induced in Group B, C and D by injecting alloxan at the dose of 120 mg/kg according to the weight of rats. Rats in group C and D were treated with S-allylcysteine [150 mg/kg], gliclazide [5 mg/kg] respectively. Random blood sugar levels of all groups were measured after weeks 1, 2 and 3. The data was analyzed by SPSS version 21.0

Results: Blood glucose levels after 2[nd] and 3[rd] week were 264.3 +/- 43.16 vs. 218.75 +/- 36.38 [p=0.0001] for SAC and 229.0 +/- 89.87 vs. 241.0 +/- 48.19 mg/dl for Gliclazide [p=0.001] while at the end of 3[rd] week results for two control groups were non-significant statistically [p-0.07 and0.09]

Conclusion: It is concluded that S-allylcysteine possess blood glucose lowering properties that are comparable to gliclazide

Hyperglycemia; Catharanthus Roseus and Nigella sativa ameliorate in alloxan induced diabetic rats

To investigate blood glucose regulaitng effects of Catharanthus Roseus [C.roseus] and Nigella sativa [N.sativa] in alloxan induced diabetic rats. Experimental study. Animal house Isra University Hyderabad. From April to Novermber 2013. 50 male rats were housed at normal température, 12 hour dark - light cycle with free access to chow and water. A single intraperitoneal bolus of alloxan [120 mg/kg] was given to induce diabetes mellitus. Glimepiride, C. roseus and N. Sativa were administered at doses of 0.1 mg/kg, 125 mg/kg and 50 g/kg respectively. Data was analyzed on SPSS version 21.0. Significant P - value was defined at

Role of magnesium sulfate in the management of acute asthma

Objectives: To determine the supportive role of magnesium sulfate in the management of acute sthmatic patients

Study design: A descriptive comparative study

Place and duration: The Study was conducted from January 2010 to June 2010 at Department of Medicine, Isra University Hospital, Hyderabad, Liaquat University Hospital, Hyderabad and Institute of Chest Diseases, Kotri, Sindh

Methodology: Acute asthmatic patients between ages of 10 to 40 years reporting to emergency department were enrolled in the study. They were divided into two groups. Groups I patients [n = 36] received oxygen inhalation, nebulization by ventoline and intravenous hydrocortisone. Group II patients [n = 37] received oxygen, ventoline nebulization, intravenous hydrocortisone and IV magnesium sulfate 25 ml /kg body weigh diluted in 100 ml of normal saline delivered over a period of 30 minutes

Results: Seventy three patients were included in this study. Baseline parameters were compared in the two groups. Both group showed significant improvement in pulse rate which was 90.9 +/- 8.46 in group I [without magnesium] and 82.3 +/- 5.11 beat per minute in group II [with magnesium]. Oxygen saturation after 120 minute was 95.9 +/- 2.02 and 96.2 +/- 1.5% in group I and group II, respectively. Similarly, after 120 minutes, PEFR was 205 L/minute in group I as compared to 237 L/minute in group II

Conclusion: This study suggested that there was benefit of adding IV magnesium sulfate to ventoline nebulization and intravenous hydrocortisone in the treatment of severe or life threatening asthma

Effect of combination of nigella sativa and trigonella foenum-graecum seeds with glibenclamide on blood sugar levels in type-2 diabetes mellitus patients

To evaluate the effect of combination of Nigella sativa and Trigonella foenum-graecum seeds with Glibenclamide on blood sugar levels in type-2 diabetes mellitus patients. Arandomized clinical trial. Diabetic outpatient clinics of Isra University Hyderabad and Liaquat University of Medical and Health Sciences Jamshoro, Pakistan from March to August 2008. For this study, Type-2 diabetic mellitus patients on Glibenclamide, who gave written consent to volunteer in the study, were randomly divided into two groups. Fifty patients in Group [A] [Control Group] remained on routine dose of Glibenclamide, while fifty patients in Group [B] [Intervention Group] were kept on a capsule containing combined powder of N-sativa and T. foenum-graecum seeds powder, in addition to their routine dose of Glibenclamide. Patients in both the groups were evaluated for a period of 3 months for fasting and random sugar levels. It was found that the blood sugar level fasting [p-value=0.003] and the random [p-value<0.001] significantly decreased in intervention group compared to control group. This study indicates that combination therapy of N. sativa and T. foenum-graecum seeds with Glibenclamide has significant effect in controlling hyperglycemia

Effect of combination therapy of Nigella sativa and trigonella foenum-graecum on body mass index in type-2 diabetes mellitus patients

Objective: To evaluate the effect of combination therapy of Nigella sativa and Trigonella foenum-graecum seeds on body mass index [BMI] in type 2 diabetic patients.

Setting: This clinical trial was conducted in the diabetic outpatient clinics of Isra University Hyderabad and Liaquat University of Medical and Health Sciences Jamshoro, from March to August 2008

Design:A randomized clinical trial

Subjects and Methods: For this study, Type 2 diabetic patients on glibenclamide, who gave written consent to volunteer in the study, were randomly divided into two groups. Fifty patients in group A [control group] were kept on glibenclamide, while 50 patients in group B [test group] were given capsules containing combined powders of N. sativa and T. foenum-graecum seeds in addition to their routine dose of glibenclamide. Patients in both groups were treated for 3 months. BMI was calculated for every patient at the start and after 3 months of therapy

Results: A favorable impact of combination therapy with N. sativa and T. foenum-graecum seeds in reducing BMI was noted in the test group in comparison to the control group, but overall differences between groups were not statistically significant

Conclusion: This study indicates that combination therapy of N. sativa and T. foenum-graecum has some effectiveness in controlling BMI in type 2 diabetic patients. Further study with an adequate sample size is recommended

Association of tea and other addicitive substances with gallstone disease in southern Sindh, Pakistan

To evaluate any association of consumption and / or use of tea, paan, supari, naas, naswar, smoking, coffee and alcohol with gallstone disease. Case - Control study The study was carried out at different hospitals of Hyderabad and adjoining areas [Liaquat University Hospital, Jamshoro, Memon Charitable Hospital, Hyderabad, Wali Bhai Rajputana Hospital, Hyderabad, Naseem Medical Center, Hyderabad, and Isra University Hospital, Hyderabad] during January 1999 to December 2001. 160 hospitalized gallstone patients and 112 patients admitted in eye wards were taken as controls [age, sex and locality matched with negative personal and family history]. The justification of taking eye patients as controls lie in the fact that these patients have no gastrointestinal problem which could be associated with gallstones. All were interviewed through a standard questionnaire developed for that purpose. The consumption and / or use of tea, paan, naas / naswaar, alcohol and coffee were common among both the groups. Smoking was found to be the main risk factor for gallstone patients. Smokers as against non-smokers were however at risk 1.89 [95% CI, 0.68-5.26, p=0.105] to develop gallstones. Similarly consumers of more than 1 cup of tea per day were seen to be at higher risk [4.07, 95% CI; 1.71-9.64, p=0.001] for gallstone disease. No association of Supari, Naas and Naswar intake was found with the occurrence of gallstones. A significant inverse association [0.49 CI; 0.34-0.5, p=0.04] of paan, [0.39 CI; 0.11-0.52, p=0.00] alcohol, and [0.36 CI; 0.24-0.98, p=0.04] coffee was found with gallstone disease. Tea consumption is positively and Paan, alcohol and coffee consumptions are negatively associated with gallstone disease in Southern Sindh, Pakistan

Hyperhomocysteinaemia and acute myocardial infarction in patients admitted at Isra Uneversity Hospital, Hyderabad

Myocardial infarction is a major consequence of coronary artery disease. Recently many reports have been suggested that hyperhomocysteinemia had an important role in myocardial infarction. Plasma homocysteine level was determined in 60 myocardial infarction patient [Group A] and 35 healthy controls [Group B]. Statistically significant difference was observed in plasma homocystine concentrations between the patients of acute myocardial infarction [Group A] and in normal healthy individuals [Group B]. The level of homocystine in patients of myocardial infraction is significantly increased when compared with controls. This indicates a strong association between hyperhomocysteinemia and acute myocardial infraction in the peoples of Hyderabad, thus showing plasma homocysteine as a risk factor for myocardial infarction

Incidence of H. pylori antibodies in patients with non ulcer dyspepsia admitted in people's medical college hospital, Nawabshah

To find out incidence of H. pylori anti bodies in non ulcer dyspepsia patients admitted in people's medical college hospital, Nawabshah. A prospective study was conducted at Department of medicine, unit III peoples medical college hospital, Nawabshah from March 2003 to Oct 2004. The patients admitted in medical unit-3 in PMCH, Nawabshah with dyspeptic symptoms. Any organic disease was excluded by upper G I T endoscopy. H. pylori antibodies were done by kit method. A total of 100 patients were taken. 60% were female and 40% were male. H. pylori antibodies were positive in 45 [45%] subjects. H. Pylori is significantly positive in non-ulcer dyspepsia patients. So every patient of non ulcer dyspepsia should be evaluated for H. Pylori antibodies