Risk factors of lymph node metastasis in low-grade endometrial carcinoma and their correlation with the prognosis of the disease / 医学研究生学报

Objective Controversies exist as to the influences of the characteristics of lymph node metastasis ( LNM) on the prognosis of low-grade endometrial carcinoma ( LGEC) .The aim of this study was to investigate the risk factors of LNM and their im-pact on the prognosis of LGEC. Methods This study included 218 LGEC patients treated by retroperitoneal lymph node dissection. We analyzed the pathologic characteristics of LNM and its relationship with the survival rate of the patients. Results LNM was ob-served in 37 of the 218 patients (16.97%), including 20 cases of pelvic (54.05%), 6 cases of para-aortic (16.22%), and 11 ca-ses of both pelvic and para-aortic ( 29.73%) LNM.Independent risk factors of LNM included deep myometrial infiltration ( OR:5.21, 95%CI:2.77-9.81), cervical stromal involvement (OR:3.15, 95% CI:1.12-8.35), lymphovascular invasion (OR:1.15, 95%CI:1.02-1.30), and abnormally high serum CA125 (OR:3.46, 95%CI:1.56-7.67) (P<0.05).There were sig-nificant differences in the 3-year survival rate between the patients with LNM and those without LNM (83.8%vs 95.0%, P<0.05) as well as in the 3-year tumor-free survival rate (73.0% vs 90.1%, P<0.05). Conclusion Deep myometrial infiltration, cervical stromal involvement, lymphovascular invasion, and abnormally high serum CA125 are the risk factors of LNM in LGEC patients, and LNM affects the prognosis of the LGEC patients.

Distribution of HLA-DRB1 allele polymorphism in the Uyghur women with family history of cervical cancer / 中华肿瘤杂志

<p><b>OBJECTIVE</b>To study the distribution of HLA-DRB1 allele polymorphism in Uyghur women with family history of cervical cancer, and provide theoretical evidence for detection and follow-up of high risk persons for cervical cancer by detection of HLA-DRB1 allele polymorphism.</p><p><b>METHODS</b>The HLA-DRB1 13 alleles were detected in 1000 Uyghur women, all from Hotan Moyu county Karsay village by using polymerase chain reaction sequence-specific oligonucleotide (PCR-SSO) assay.</p><p><b>RESULTS</b>The frequencies of HLA-DRB1*15 in women with family history of cervical cancer (17.3%), mother (18.0%) and other relatives except mother (17.0%) who had suffered from cervical cancer were significantly higher than that in the control group (9.7%, all P < 0.05). The frequencies of HLA-DRB1*04 in women with family history (16.8%) and other relatives except mother (20.7%) were significantly higher than that in the control group (12.7%, all P < 0.05). The frequencies of HLA-DRB1*03 in women with family history (2.6%) and other relatives except mother (1.1%) were significantly lower than that in the control group (6.3%, all P < 0.01). The frequencies of HLA-DRB1*12 in women with family history of cervical cancer (2.3%) and mother suffered from cervical cancer (1.5%) were significantly lower than that in the control group (5.7%, all P < 0.05). The frequencies of HLA-DRB1*14 in women with family history of cervical cancer (5.4%) and mother who suffered from cervical cancer (3.0%) were significantly lower than that in the control group (8.4%, all P < 0.05).</p><p><b>CONCLUSIONS</b>There are similarity and difference in distribution of HLA-DRB1 allele polymorphisms between the Uyghur women with family history of cervical cancer from Hotan Moyu county and those from southern Xingjiang area. In general, the distribution of HLA-DRB1 allele polymorphism in women with family history of cervical cancer is similar to that reported in abroad. The results of this study support the role of susceptible and protective HLA gene detection in screening high risk persons for this cancer among Uyghur women from cervical cancer high risk areas in Xinjiang.</p>

Establishment and characterization of a new carcinoma cell line from uterine cervix of Uyghur women / 中华病理学杂志

<p><b>OBJECTIVE</b>To establish a uterine cervical carcinoma cell line of Uyghur ethnical background and to evaluate the related biological characteristics for future biomedical investigations of diseases in the Uyghur population.</p><p><b>METHODS</b>Poorly-differentiated squamous cell carcinoma specimens of Uyghur patients were obtained and cultured in vitro by enzymatic digestion method, followed by continuous passaging to reach a stable growth determined by cell viability and growth curve. Morphological study, cell cycling and chromosomal analysis were performed. Tumorigenesis study was conducted by inoculation of nude mice. Biomarker (CK17, CD44, Ki-67, CK14 and vimentin) expression was detected by immunofluorescence and immunocytochemical techniques.</p><p><b>RESULTS</b>A cervical carcinoma cell line was successfully established and maintained for 12 months through 70 passages. The cell line had a stable growth with a population doubling time of 51.9 h. Flask method and double agar-agar assay showed that the cell line had colony-forming rates of 32.5% and 15.6%, respectively. Ultrastructural evaluation demonstrated numerous cell surface protrusions or microvilli, a large number of rod-shape structures in cytoplasm, typical desmosomes and nuclear atypia. Chromosomal analysis revealed human karyotype with the number of chromosomes per cell varying from 32 - 97 with a majority of 54 - 86 (60.3%). Xenogeneic tumors formed in nude mice showed histological structures identical to those of the primary tumor. The cells had high expression of CK17, CD44, Ki-67 and vimentin but no CK14 expression.</p><p><b>CONCLUSIONS</b>A cervical carcinoma cell line from a female Uyghur patient is successfully established. The cell line has the characteristics of human cervical squamous cell carcinoma, and it is stable with maintaining the characteristic biological and morphological features in vitro for more than 12 months, therefore, qualified as a stable cell line for further biomedical research.</p>

The spectrum of HPV infection in Xinjiang Uighur women with cervical cancer / 肿瘤

Objective: To investigate the difference in distribution of subtypes of Human papilloma virus (HPV) between cervical cancer patients and normal controls of Xinjiang Uighur women and analyze the HPV spectrum in Xinjiang Uighur women suffered from cervical cancer. Methods: Three hundred and thirty Uighur women with cervical cancer and one hundred normal healthy women were recruited in this study. Flow-through hybridization and gene chip (HybriMax) technology was used to detect the twenty one subtypes of HPV. Results: (1) The positive rate of HPV infection including simple infection and multiple infection was 85.15% (281/300) and 7.0% (7/100) in cervical cancer group and control group, respectively. The positive rate of HPV16 infection was 94.31% indicating it was the most common infection in HPV-positive cervical cancer patients. The infection rate was 5.34% for HPV18, 3.91% for HPV68, 2.49% for HPV45, 2.49% for HPV58, 2.14% for HPV39, 1.07% for HPV31, 1.07 for HPV56, and 0.36 for HPV59. There was significant difference in the total infection rate and the HPV16 infection rate between cervical cancer patients and controls (P0.05 ). Although the positive rate of multiple infection of HPV tended to increase but the difference was not significant. Conclusion: HPV16 infection was the most common in cervical cancer patients and normal controls of Xinjiang Uighur women. The next most common types of HPV were HPV18 and HPV68. Xinjiang Uighur women had the relatively higher risk of suffering HPV68 infection indicating the specificity of HPV infection in Uighur women.

Distribution of human papillomavirus and its subtypes in tissue samples of Uigur cervical cancer from southern Xinjiang / 中国癌症杂志

Purpose:To study the association between human papillomavirus (HPV) and cervical cancer in Uigur women from southern Xinjiang where there is a high risk of cervical cancer.Methods:HPV-C,HPV16,HPV18 and HPV6/ 11 were detected in 40 fresh tissues of cervical cancer and 40 tissues of normal cervix by using polymerase chain reaction technique.Results:The positive rate of HPV-C,HPV16,HPV18 and HPV6/11 in fresh tissue of cervix cancer were 87. 5%,72.5%,10.0% and 0,respectively;The positive rate of HPV-C,HPV16,HPV18 and HPV6/11 in normal cervix were20.0%,5.0%,0 and 7.5%,HPV16 was the most common type in HPV-C positive patients with the rate of 82.8 %. The positive rate of HPV-C and HPV16 in cervical cancer were significantly higher than that in control groups;The positive rate of HPV16 in squamous cancer of cervix was significantly higher than that in adenocarcinomas of cervix;while the posi- tive rate of HPV 18 in adenocarcinomas of cervix was significantly higher than that in squamous cancer of cervix(P0.05).Conclusions:HPV16 infection plays an important role in the carcinogenesis of cervical cancer of Uigur women from Southern Xinjiang,HPV16 infection is closely correlated with squamous carcinomas of cervix,while HPV18 infection is closely correlated with adenocarcinomas of cervix.The positive rate of HPV-C and HPV16 have no correlation with clinical stages and histological grade of cervical carcinomas.