Résumé
The objective of this study is to elucidate the clinical significance of DNA flow cytometry and study its impact on monitoring the progression of gastric precancerous lesions in patients with gastric dyspepsia and to correlate between endoscopic and histopathological findings with results of DNA flow cytometry. A total of 45 cases[28 males with mean age 42.5 years and 17 females with mean of 36.5 years] underwent upper gastrointestinal endoscopy. Based on the endoscopic appearance, patients under study were classified into 5 cases with endoscopic normal mucosa [EN] as controls, 29 cases with endoscopic gastritis [EG], 9 cases with duodenal ulcer [DU] and 2 cases with gastric ulcer [GU], two antral biopsies were taken for histopathology and DNA flow cytometry. Chronic gastritis [CG] was present in two of EN case. In DU patients, CG was present in 9 cases and associated with H. pylori in 5 cases and with metaplasia in one of these cases. While in GU patients, CG was present in all cases; but one case was associated with dysplasia. In EG patient, CG was present in all cases and it was associated with metaplasia in 4 of these cases. One of the endoscopic normal cases revealed DNA aneuploidy in specimens with CG. The incidence of aneuploidy increases as the endoscopic findings changes from EG [10.4%], DU [22.2%] to GU [50%] and as histopathological changes progresses from chronic active gastritis with H. pylori [7.7%], chronic gastritis with metaplasia [20%] and dysplasia [100%]